ROCK1 蛋白, Human (Sf9, His)

The ROCK1 protein is a key kinase that regulates the actin cytoskeleton, cell polarity, smooth muscle contraction, and multiple cellular functions. It controls stress fibers, focal adhesion formation, neurite retraction, and cell motility by phosphorylating substrates such as DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1, and PPP1R12A. ROCK1 Protein, Human (Sf9, His) is the recombinant human-derived ROCK1 protein, expressed by Sf9 insect cells , with N-8*His labeled tag. The total length of ROCK1 Protein, Human (Sf9, His) is 410 a.a., .

ROCK1 蛋白是一种关键激酶，对肌动蛋白细胞骨架和细胞极性具有关键的调节影响。ROCK1 具有广泛的细胞功能，通过多种底物（如 DAPK3、GFAP、LIMK1、LIMK2）的磷酸化，复杂地控制平滑肌收缩、肌动蛋白细胞骨架组织、应力纤维和粘着斑形成、神经突回缩、细胞粘附和运动。MYL9/MLC2、TPPP、PFN1 和 PPP1R12A。它与 FHOD1 协同诱导 SRC 依赖性非凋亡质膜起泡并磷酸化 JIP3，从而在 UVB 诱导的应激期间调节 JNK 向 JIP3 的募集。作为炎症细胞迁移的抑制因子，ROCK1 影响 PTEN 磷酸化和稳定性，同时充当 VEGF 诱导的血管生成内皮细胞激活的负调节因子。ROCK1 在中心体定位和有丝分裂退出中发挥着至关重要的作用，在红细胞终末分化、足细胞运动抑制、促进角质形成细胞终末分化以及在基质组装过程中促进成骨细胞压实方面发挥作用，从而有助于成骨细胞矿化。此外，ROCK1 可能通过诱导肌动球蛋白束组装影响眼睑和腹侧体壁闭合。ROCK1 的多种作用强调了其对细胞生理学的多方面影响，并强调了其在各种细胞过程中的调节意义。

Human

Sf9 insect cells

N-8*His

Q13464 (S6-R415)

6093

50 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT. pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.