Siglec-2/CD22 蛋白, Human (FITC, HEK293, Fc)

Siglec-2/CD22 Protein mediates B-cell interactions, potentially guiding B-cell localization within lymphoid tissues. Recognizing sialylated glycoproteins, especially alpha-2,6-linked sialic acid, it engages in cis interactions on the cell surface. During the immune response, ligand-induced tyrosine phosphorylation suggests its role in B-cell antigen receptor signaling. Siglec-2/CD22 acts positively through Src kinases and inhibits by recruiting phosphatases. Existing as a monomer or heterodimer, its interactions with key molecules highlight a pivotal role in complex B-cell signaling. Further research is essential for precise molecular pathways and functional consequences. Siglec-2/CD22 Protein, Human (FITC, HEK293, Fc) is the recombinant human-derived Siglec-2/CD22 protein, expressed by HEK293, with C-hFc labeled tag. The total length of Siglec-2/CD22 Protein, Human (FITC, HEK293, Fc) is 668 a.a., with molecular weight of 130-140 kDa.

Siglec-2/CD22 蛋白是 B 细胞相互作用中的关键介质，可能在 B 细胞在淋巴组织内的定位中发挥作用。它以其结合唾液酸化糖蛋白（包括 CD45）的能力而闻名，它表现出对 α-2,6-连接唾液酸的偏好。唾液酸识别位点可能被同一细胞表面上唾液酸的顺式相互作用所掩盖。在免疫反应过程中，配体诱导的酪氨酸磷酸化表明其参与 B 细胞抗原受体信号传导的调节。该蛋白的多方面作用包括通过与 Src 家族酪氨酸激酶相互作用进行正向调节，同时作为抑制性受体，通过其 SH2 结构域招募细胞质磷酸酶，通过信号分子的去磷酸化来阻断信号转导。Siglec-2/CD22 主要作为异构体 CD22-β 的单体存在，也可以与较短的异构体形成异二聚体。它与 PTPN6/SHP-1、LYN、SYK、PIK3R1/PIK3R2、PLCG1、GRB2、INPP5D 和 SHC1 等关键分子之间复杂的相互作用，特别是在磷酸化时，突显了其在协调 B 细胞内复杂信号网络中的关键作用。进一步的研究对于揭示 Siglec-2/CD22 在 B 细胞调节和免疫反应中的精确分子途径和功能后果至关重要。

Human

HEK293

C-hFc

P20273 (D20-R687)

933  [NCBI]

130-140 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.