TGFBR1/ALK-5 蛋白, Human (HEK293, mFc-Avi)

TGFBR1/ALK-5 proteins cooperate with TGFBR2 to form dedicated receptors for TGFB1, TGFB2, and TGFB3, transmitting signals and coordinating different physiological and pathological processes. It induces cell cycle arrest, modulates mesenchymal cell dynamics, contributes to wound healing, and affects immunosuppression and carcinogenesis. TGFBR1/ALK-5 Protein, Human (HEK293, mFc-Avi) is the recombinant human-derived TGFBR1/ALK-5 protein, expressed by HEK293 , with C-Avi, C-mFc labeled tag. The total length of TGFBR1/ALK-5 Protein, Human (HEK293, mFc-Avi) is 92 a.a., with molecular weight of 50-60 kDa.

跨膜丝氨酸/苏氨酸激酶 TGFBR1 (ALK-5) 与 TGF-β II 型丝氨酸/苏氨酸激酶受体 TGFBR2 协作，形成 TGF-β 细胞因子的专用受体，包括 TGFB1、TGFB2 和 TGFB3。作为信号转导器，TGFBR1 介导 TGFB1、TGFB2 和 TGFB3 信号从细胞表面到细胞质的传递，从而协调多种生理和病理过程。这些包括上皮细胞和造血细胞的细胞周期停滞、间充质细胞增殖和分化的控制、伤口愈合、细胞外基质的产生、免疫抑制和致癌作用。该受体复合物由 2 个 TGFBR1 和 2 个 TGFBR2 分子对称地与细胞因子二聚体结合组成，导致 TGFBR1 被组成型活性 TGFBR2 磷酸化和激活。激活的 TGFBR1 磷酸化 SMAD2，导致其与受体解离并与 SMAD4 相互作用。由此产生的 SMAD2-SMAD4 复合物易位到细胞核，在细胞核中调节 TGF-β 调节基因的转录，构成典型的 SMAD 依赖性 TGF-β 信号级联。此外，TGFBR1 还参与非典型的、不依赖 SMAD 的 TGF-β 信号通路。例如，它诱导 TRAF6 自动泛素化，导致 MAP3K7 泛素化和激活，引发细胞凋亡。TGFBR1 还通过涉及 PARD6A 磷酸化和激活的 SMAD 独立信号通路来调节上皮细胞向间质细胞的转变。

1.Human TGFBR1, mFc Tag captured on CM5 Chip via Protein A can bind Human Mature TGF beta 1, No Tag with an affinity constant of 0.12 μM as determined in SPR assay (Biacore T200).
2.Measured  by its binding ability in a functional ELISA. When Recombinant Human TGF-beta   RII is immobilized at 1 μg/mL (100 μL/well), it binds Recombinant  Human TGF-beta RI in the presence of TGF-beta 1. The concentration of  rhTGF-beta RI that produces 50% of the optimal binding response is  approximately 1.639 μg/mL.

Human

HEK293

C-Avi;C-mFc

P36897-1 (L34-E125)

7046  [NCBI]

LQCFCHLCTKDNFTCVTDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGSVTTTYCCNQDHCNKIELPTTVKSSPGLGPVE

50-60 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.