2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Tissue alpha-L-Fucosidase 蛋白, Mouse (His-SUMO)

Tissue alpha-L-Fucosidase 蛋白, Mouse (His-SUMO)

目录号: HY-P71621
产品使用指南

组织 α-L-岩藻糖苷酶蛋白是一种酶实体，可水解糖蛋白碳水化合物部分上的 α-1,6-连接岩藻糖残基。Tissue alpha-L-Fucosidase 蛋白, Mouse (His-SUMO) 是重组的 组织 α-L-岩藻糖苷酶蛋白，由 E. coli 表达，带有 N-His, N-SUMO 标签。Tissue alpha-L-Fucosidase 蛋白, Mouse (His-SUMO) 全长 435 个氨基酸，分子量约为 ~66.6 kDa。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

Tissue alpha-L-Fucosidase Proteinas, an enzymatic entity, crucially hydrolyzes alpha-1,6-linked fucose residues on glycoproteins' carbohydrate moieties. Tissue alpha-L-Fucosidase Protein, Mouse (His-SUMO) is the recombinant mouse-derived Tissue alpha-L-Fucosidase protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Tissue alpha-L-Fucosidase Protein, Mouse (His-SUMO) is 435 a.a., with molecular weight of ~66.6 kDa.
研究背景

组织 α-L-岩藻糖苷酶作为一种酶实体，在糖蛋白碳水化合物部分中连接到还原端 N-乙酰葡糖胺的 α-1,6 连接岩藻糖残基的水解中起着至关重要的作用。
种属

Mouse
表达系统

E. coli
标签

N-His;N-SUMO
蛋白编号

Q99LJ1 (18L-452N)
基因 ID
蛋白结构
N-term
6*His-SUMO
FUCA1 (18L-452N)
Accession # Q99LJ1
C-term
同用名
Fuca1; FucaTissue alpha-L-fucosidase; EC 3.2.1.51; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; Alpha-L-fucosidase 1
氨基酸序列

LAPRRFTPDWQSLDSRPLPSWFDEAKFGVFVHWGVFSVPAWGSEWFWWHWQGDRMPAYQRFMTENYPPGFSYADFAPQFTARFFHPDQWAELFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLVGELGAAVRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVRAKTMPELYDLVNSYKPDLIWSDGEWECPDTYWNSTSFLAWLYNDSPVKDEVIVNDRWGQNCSCHHGGYYNCQDKYKPQSLPDHKWEMCTSMDRASWGYRKDMTMSTIAKENEIIEELVQTVSLGGNYLLNIGPTKDGLIVPIFQERLLAVGKWLQINGEAIYASKPWRVQSEKNKTVVWYTTKNATVYATFLYWPENGIVNLKSPKTTSATKITMLGLEGDLSWTQDPLEGVLISLPQLPPTVLPVEFAWTLKLTKVN

分子量

Approximately 66.6 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Tissue alpha-L-Fucosidase Protein, Mouse (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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