TPP1 蛋白, Human (HEK293, His)

TPP1, a lysosomal serine protease, acts as a non-specific lysosomal peptidase, displaying tripeptidyl-peptidase I activity in lysosomal protein degradation. Its function involves generating tripeptides from the breakdown products of other lysosomal proteinases, emphasizing its role in processing and hydrolyzing peptides within the lysosomal environment. Notably, TPP1 requires substrates with an unsubstituted N-terminus. TPP1 Protein, Human (HEK293, His) is the recombinant human-derived TPP1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TPP1 Protein, Human (HEK293, His) is 544 a.a., with molecular weight of ~78.75 kDa.

TPP1 是一种溶酶体丝氨酸蛋白酶，具有三肽基肽酶 I 活性，其参与溶酶体蛋白质降解就证明了这一点。虽然其精确的底物特异性尚未完全阐明，但 TPP1 被认为具有非特异性溶酶体肽酶的功能，从其他溶酶体蛋白酶产生的分解产物中产生三肽。值得注意的是，TPP1 需要具有未取代的 N 末端的底物，这进一步凸显了它在溶酶体环境中加工和水解肽中的作用。

Measured by its ability to cleave the fluorogenic peptide substrate Ala-Ala-Phe-AMC that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 6174.46 pmol/min/µg.

Human

HEK293

C-6*His

AAH14863.1 (S20-P563)

1200  [NCBI]

SYSPEPDQRRTLPPGWVSLGRADPEEELSLTFALRQQNVERLSELVQAVSDPSSPQYGKYLTLENVADLVRPSPLTLHTVQKWLLAAGAQKCHSVITQDFLTCWLSIRQAELLLPGAEFHHYVGGPTETHVVRSPHPYQLPQALAPHVDFVGGLHRFPPTSSLRQRPEPQVTGTVGLHLGVTPSVIRKRYNLTSQDVGSGTSNNSQACAQFLEQYFHDSDLAQFMRLFGGNFAHQASVARVVGQQGRGRAGIEASLDVQYLMSAGANISTWVYSSPGRHEGQEPFLQWLMLLSNESALPHVHTVSYGDDEDSLSSAYIQRVNTELMKAAARGLTLLFASGDSGAGCWSVSGRHEFRPTFPASSPYVTTVGGTSFQEPFLITNEIVDYISGGGFSNVFPRPSYQEEAVTKFLSSSPHLPPSSYFNASGRAYPDVAALSDGYWVVSNRVPIPWVSGTSASTPVFGGILSLINEHRILSGRPPLGFLNPRLYQQHGAGLFDVTRGCHESCLDEEVEGQGFCSGPGWDPVTGWGTPNFPALLKTLLNP

Approximately 78.75 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM CaCl₂, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.