SPD304 dihydrochloride 

SPD304 dihydrochloride is a selective TNF-α inhibitor, which promotes dissociation of TNF trimers and therefore blocks the interaction of TNF and its receptor. SPD304 has an IC₅₀ of 22 μM for inhibiting in vitro TNF receptor 1 (TNFR1) binding to TNF-α^[1][2].

IC50: 22 µM (TNFα)^[1].

SPD304 (2 μM) 显著提高 aHSC 的生存能力，减少脂质氢氧化物的产生，并增加细胞内 GSH。与单独使用 GA 相比，GA (75 μM) 和 SPD304 (2 μM) 的共同处理下调 TRADD 几乎 2 倍 (无抑制剂与有抑制剂) 和 p-RIP3 1.4-倍，并促进Caspase 8 激活^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

由于 SPD304 的高毒性不能在体内使用^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

620.53

C₃₂H₃₄Cl₂F₃N₃O₂

1049741-03-8

固体

White to off-white

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

• [1]. Molly M. He, et al. Small-Molecule Inhibition of TNF-α. Science 11 Nov 2005.

  [2]. Alexiou P, et al. Rationally designed less toxic SPD-304 analogs and preliminary evaluation of their TNF inhibitory effects. Arch Pharm (Weinheim). 2014 Nov;347(11):798-805.  [Content Brief]

  [3]. Mouhsine H, et al. Identification of an in vivo orally active dual-binding protein-protein interaction inhibitor targeting TNFα through combined in silico/in vitro/in vivo screening. Sci Rep. 2017 Jun 13;7(1):3424.  [Content Brief]

  [4]. Gallic acid induces necroptosis via TNF-α signaling pathway in activated hepatic stellate cells. Chang YJ, et al. PLoS One. 2015 Mar 27;10(3):e0120713.  [Content Brief]