2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. TLR7/8 agonist 1

TLR7/8 agonist 1 

目录号: HY-103698 纯度: 97.33%
COA 产品使用指南

摩尔计算器

TLR7/8 agonist 1是一种 toll 样受体 (TLR7)/TLR8 的双重激动剂。

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TLR7/8 agonist 1 Chemical Structure

TLR7/8 agonist 1 Chemical Structure

CAS No. : 1258457-59-8

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1430
In-stock
1 mg ￥520
In-stock
5 mg ￥1300
In-stock
10 mg ￥2100
In-stock
25 mg ￥4000
In-stock
50 mg ￥6000
In-stock
100 mg   询价  
200 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of TLR7/8 agonist 1:

TLR7/8 agonist 1 相关抗体

Top Publications Citing Use of Products

查看 Toll-like Receptor (TLR) 亚型特异性产品:

查看所有亚型
TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

TLR7/8 agonist 1 is a toll-like receptor (TLR7)/TLR8 dual-agonistic imidazoquinoline.

IC50 & Target

TLR7

 

TLR8

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
HEK293 EC50
528 nM
Compound: 1
Agonist activity at human TLR7 transfected in HEK reporter cells assessed as NF-kappaB induction by specific secreted alkaline phosphatase gene assay
Agonist activity at human TLR7 transfected in HEK reporter cells assessed as NF-kappaB induction by specific secreted alkaline phosphatase gene assay
[PMID: 21549593]
HEK293 EC50
55 nM
Compound: 1
Agonist activity at human TLR8 transfected in HEK293 cells assessed as activation of NF-kB by reporter gene assay
Agonist activity at human TLR8 transfected in HEK293 cells assessed as activation of NF-kB by reporter gene assay
[PMID: 26351878]
体外研究
(In Vitro)

TLR7/8 agonist 1 (Compound 5d) shows prominent immunostimulatory activities. TLR7/8 agonist 1 serves as a convenient precursor for the covalent attachment of fluorophores without significant loss of activity.TLR7/8 agonist 1 retains TLR7-agonistic activity with an EC50 of 20 nM. TLR7/8 agonist 1 is covalently coupled directly to commercially-available fluorescein isothiocyanate and rhodamine B isothiocyanate[1]. TLR7/8 agonist 1 (Compound 1) shows substantially different agonistic potencies in human TLR7 (50 nM) and TLR8 (55 nM) primary screens[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

TLR7/8 agonist 1 相关抗体:
分子量

359.47

Formula

C22H25N5
CAS 号
性状

固体

颜色

Off-white to light yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 11.11 mg/mL (30.91 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7819 mL 13.9094 mL 27.8187 mL
5 mM 0.5564 mL 2.7819 mL 5.5637 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献
Kinase Assay
[2]

The induction of NF-κB is quantified using human TLR-2, TLR3, TLR4, TLR5, TLR7, TLR8, TLR9, and NOD-1/NOD-2-specific, rapid-throughput, liquid handler-assisted reporter gene assays. HEK293 cells stably co-transfected with the appropriate hTLR (or NOD) and secreted alkaline phosphatase (sAP) are maintained in HEK-Blue Selection medium. Stable expression of secreted alkaline phosphatase (sAP) under control of NF-κB/AP-1 promoters is inducible by appropriate TLR/NOD agonists, and extracellular sAP in the supernatant is proportional to NF-κB induction. Reporter cells are incubated at a density of ~105 cells/mL in a volume of 80 μL/well, in 384-well, flat-bottomed, cell culture-treated microtiter plates in the presence of graded concentrations of stimuli. sAP is assayed spectrophotometrically using an alkaline phosphatase-specific chromogen (present in HEK-detection medium) at 620 nm[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[2]

Fresh human peripheral blood mononuclear cells (hPBMC) are isolated from human blood obtained by venipuncture with informed consent and as per institutional guidelines on Ficoll–Hypaque gradients. Aliquots of PBMCs (105 cells in 100 μL/well) are stimulated for 12 h with graded concentrations of test compounds (e.g., TLR7/8 agonist 1; 0.1, 1, 10, and 100 μg/mL). Supernatants are isolated by centrifugation and are assayed in duplicates using analyte-specific multiplexed cytokine/chemokine bead array assays[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

TLR7/8 agonist 1 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7819 mL 13.9094 mL 27.8187 mL 69.5468 mL
5 mM 0.5564 mL 2.7819 mL 5.5637 mL 13.9094 mL
10 mM 0.2782 mL 1.3909 mL 2.7819 mL 6.9547 mL
15 mM 0.1855 mL 0.9273 mL 1.8546 mL 4.6365 mL
20 mM 0.1391 mL 0.6955 mL 1.3909 mL 3.4773 mL
25 mM 0.1113 mL 0.5564 mL 1.1127 mL 2.7819 mL
30 mM 0.0927 mL 0.4636 mL 0.9273 mL 2.3182 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TLR7/8 agonist 11258457-59-8Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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产品名称：
TLR7/8 agonist 1
目录号：
HY-103698
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