trans-ACPD (Synonyms: Trans-(±)-ACP)

目录号: HY-19434 纯度: ≥98.0%: FAQs
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trans-ACPD 是一种代谢型受体激动剂，促进钙离子活动及产生内向电流。

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trans-ACPD Chemical Structure

CAS No. : 67684-64-4

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规格	价格	是否有货
Free Sample (0.1 - 0.2 mg)		Apply now
10 mM * 1 mL in DMSO	￥695	In-stock
1 mg	￥365	In-stock
5 mg	￥830	In-stock
10 mg	￥1304	In-stock
25 mg	￥2608	In-stock
50 mg	￥3913	In-stock
100 mg	￥5870	In-stock
200 mg		询价
500 mg		询价

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Other Forms of trans-ACPD:

(1S,3R)-ACPD 询价
cis-ACPD 询价

trans-ACPD 相关产品

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mGluR1 mGluR2 mGluR3 mGluR4 mGluR5 mGluR6 mGluR7 mGluR8 mGluR

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

trans-ACPD, a metabotropic receptor agonist, produces calcium mobilization and an inward current in cultured cerebellar Purkinje neurons.

IC₅₀ & Target^[1]

mGluR

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	> 100 μM Compound: 16	Stimulation of [3H]phosphatidylinositol accumulation by rat Metabotropic glutamate receptor 4 co-expressed with Gqi9 protein in HEK 293 cells; Inactive Stimulation of [3H]phosphatidylinositol accumulation by rat Metabotropic glutamate receptor 4 co-expressed with Gqi9 protein in HEK 293 cells; Inactive	[PMID: 15801843]
HEK293	EC₅₀	> 1000 μM Compound: 1/ (1S,3R -)-ACPD	Agonist activity against Metabotropic glutamate receptor 4 expressed in HEK 293 cells was evaluated by measuring total inositol phosphate accumulation Agonist activity against Metabotropic glutamate receptor 4 expressed in HEK 293 cells was evaluated by measuring total inositol phosphate accumulation	[PMID: 9301676]
HEK293	EC₅₀	18 μM Compound: 1/ (1S,3R -)-ACPD	Agonist activity against Metabotropic glutamate receptor 2 expressed in HEK 293 cells was evaluated by measuring total inositol phosphate accumulation Agonist activity against Metabotropic glutamate receptor 2 expressed in HEK 293 cells was evaluated by measuring total inositol phosphate accumulation	[PMID: 9301676]
HEK293	EC₅₀	9.3 μM Compound: 1/ (1S,3R -)-ACPD	Agonist activity against Metabotropic glutamate receptor 1 expressed in HEK 293 cells was evaluated by measuring total inositol phosphate accumulation Agonist activity against Metabotropic glutamate receptor 1 expressed in HEK 293 cells was evaluated by measuring total inositol phosphate accumulation	[PMID: 9301676]

体外研究
(In Vitro)

兴奋性氨基酸 (EAA) 类似物激活与磷酸肌醇 (PI) 水解增加相关的受体。在这些研究中，从新生大鼠 (6-11 天大) 制备海马切片，以表征 EAA 类似物对这些受体的影响。引起半数最大刺激所需的反式 ACPD 浓度 (EC₅₀ 值) 为 51 μM。DL-2-Amino-3-phosphonopropionate (DL-AP3) 也可作为 PI 水解的抑制剂，由鹅膏蕈氨酸、使君子氨酸和反式 ACPD 刺激 (IC₅₀ 值为 480-850 μM)^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

与鞘内注射生理盐水的动物相比，鞘内注射 NMDA、红藻氨酸和反式 ACPD、TNF-α 或 IL-1β 会导致小鼠出现显著 (p<0.001) 的咬合行为。在所有组中，与用盐水 (10 mL/kg，ip) 处理的小鼠相比，用 GM (100 mg/kg，ip) 进行全身预处理显著 (p<0.001) 减少了咬人行为。GM 对促炎细胞因子和 NMDA 的影响最大，抑制百分比如下：TNF-α (92±7%)、IL-1β (91±5%)、NMDA (69±1%)、和反式 ACPD (71±12%)。相比之下，在相同剂量下，GM对红藻氨酸介导的咬合反应没有显著影响^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

173.17

Formula

C₇H₁₁NO₄

CAS 号

67684-64-4

性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (288.74 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H₂O 中的溶解度 : 3.57 mg/mL (20.62 mM; 超声助溶)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	5.7747 mL	28.8737 mL	57.7474 mL
5 mM	1.1549 mL	5.7747 mL	11.5495 mL
10 mM	0.5775 mL	2.8874 mL	5.7747 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.44 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.44 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 5 mg/mL (28.87 mM); 澄清溶液; 超声助溶 (<60°C)

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: ≥98.0%

选择批次：

Data Sheet (646 KB) SDS (392 KB)

COA (193 KB) HNMR (246 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Linden DJ, et al. Trans-ACPD, a metabotropic receptor agonist, produces calcium mobilization and an inward current in cultured cerebellar Purkinje neurons. J Neurophysiol. 1994 May;71(5):1992-8. [Content Brief]

[2]. Littman L, et al. Multiple mechanisms for inhibition of excitatory amino acid receptors coupled to phosphoinositide hydrolysis. J Neurochem. 1992 Nov;59(5):1893-904. [Content Brief]

[3]. Córdova MM, et al. Polysaccharide glucomannan isolated from Heterodermia obscurata attenuates acute and chronic pain in mice. Carbohydr Polym. 2013 Feb 15;92(2):2058-64. [Content Brief]

Animal Administration ^[3]	Mice^[3] Male Swiss mice (25-35 g) are used. Intrathecal injections are given to fully conscious mice. Briefly, the animals are manually restrained, and a 30-gauge needle connected by a polyethylene tube to a 25 μL Hamilton gas-tight syringe is inserted through the skin and between the vertebrae into the subdural space of the L5-L6 spinal segments. Intrathecal injections (5 μL/site) are administered over a period of 5 s. Biting behaviour is defined as a single head movement directed at the flanks or hind limbs, resulting in contact of the animal's snout with the target organ. The nociceptive response is elicited by NMDA (450 pmol/site, a selective agonist of the NMDA glutamatergic ionotropic receptor), kainate (110 pmol/site, a selective agonist of the kainate subtype of glutamatergic ionotropic receptors), and trans-ACPD (50 nmol/site, a non-selective agonist of metabotropic glutamate receptors, which is active at group I and group II), TNF-α (0.1 pmol/site) and IL-1β (1 pmol/site) or saline (5 μL/site, i.t.). The amount of time the animal spent biting or licking the caudal region is taken as evidence of nociception and is evaluated following local post injections of the following agonists: NMDA (5 min), kainate (4 min), and trans-ACPD TNF-α, and IL-1β (15 min). Animals received GM (100 mg/kg, i.p.) 0.5 h before intrathecal injection of 5 μL of the drugs, while control animals received a similar volume of saline (10 mL/kg, i.p.). MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Linden DJ, et al. Trans-ACPD, a metabotropic receptor agonist, produces calcium mobilization and an inward current in cultured cerebellar Purkinje neurons. J Neurophysiol. 1994 May;71(5):1992-8. [Content Brief] [2]. Littman L, et al. Multiple mechanisms for inhibition of excitatory amino acid receptors coupled to phosphoinositide hydrolysis. J Neurochem. 1992 Nov;59(5):1893-904. [Content Brief] [3]. Córdova MM, et al. Polysaccharide glucomannan isolated from Heterodermia obscurata attenuates acute and chronic pain in mice. Carbohydr Polym. 2013 Feb 15;92(2):2058-64. [Content Brief]

Animal Administration
^[3]

Mice^[3]
Male Swiss mice (25-35 g) are used. Intrathecal injections are given to fully conscious mice. Briefly, the animals are manually restrained, and a 30-gauge needle connected by a polyethylene tube to a 25 μL Hamilton gas-tight syringe is inserted through the skin and between the vertebrae into the subdural space of the L5-L6 spinal segments. Intrathecal injections (5 μL/site) are administered over a period of 5 s. Biting behaviour is defined as a single head movement directed at the flanks or hind limbs, resulting in contact of the animal's snout with the target organ. The nociceptive response is elicited by NMDA (450 pmol/site, a selective agonist of the NMDA glutamatergic ionotropic receptor), kainate (110 pmol/site, a selective agonist of the kainate subtype of glutamatergic ionotropic receptors), and trans-ACPD (50 nmol/site, a non-selective agonist of metabotropic glutamate receptors, which is active at group I and group II), TNF-α (0.1 pmol/site) and IL-1β (1 pmol/site) or saline (5 μL/site, i.t.). The amount of time the animal spent biting or licking the caudal region is taken as evidence of nociception and is evaluated following local post injections of the following agonists: NMDA (5 min), kainate (4 min), and trans-ACPD TNF-α, and IL-1β (15 min). Animals received GM (100 mg/kg, i.p.) 0.5 h before intrathecal injection of 5 μL of the drugs, while control animals received a similar volume of saline (10 mL/kg, i.p.).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Linden DJ, et al. Trans-ACPD, a metabotropic receptor agonist, produces calcium mobilization and an inward current in cultured cerebellar Purkinje neurons. J Neurophysiol. 1994 May;71(5):1992-8. [Content Brief]

[2]. Littman L, et al. Multiple mechanisms for inhibition of excitatory amino acid receptors coupled to phosphoinositide hydrolysis. J Neurochem. 1992 Nov;59(5):1893-904. [Content Brief]

[3]. Córdova MM, et al. Polysaccharide glucomannan isolated from Heterodermia obscurata attenuates acute and chronic pain in mice. Carbohydr Polym. 2013 Feb 15;92(2):2058-64. [Content Brief]

trans-ACPD 相关分类

Metabolic Disease

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.7747 mL	28.8737 mL	57.7474 mL	144.3685 mL
	5 mM	1.1549 mL	5.7747 mL	11.5495 mL	28.8737 mL
	10 mM	0.5775 mL	2.8874 mL	5.7747 mL	14.4368 mL
	15 mM	0.3850 mL	1.9249 mL	3.8498 mL	9.6246 mL
	20 mM	0.2887 mL	1.4437 mL	2.8874 mL	7.2184 mL
DMSO	25 mM	0.2310 mL	1.1549 mL	2.3099 mL	5.7747 mL
	30 mM	0.1925 mL	0.9625 mL	1.9249 mL	4.8123 mL
	40 mM	0.1444 mL	0.7218 mL	1.4437 mL	3.6092 mL
	50 mM	0.1155 mL	0.5775 mL	1.1549 mL	2.8874 mL
	60 mM	0.0962 mL	0.4812 mL	0.9625 mL	2.4061 mL
	80 mM	0.0722 mL	0.3609 mL	0.7218 mL	1.8046 mL
	100 mM	0.0577 mL	0.2887 mL	0.5775 mL	1.4437 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

trans-ACPD67684-64-4Trans-(±)-ACPmGluRMetabotropic glutamate receptorsInhibitorinhibitorinhibit

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trans-ACPD (Synonyms: Trans-(±)-ACP)

Customer Review

Other Forms of trans-ACPD: