2. Cell Cycle/DNA Damage Apoptosis
  3. Deubiquitinase Apoptosis
  4. USP7-IN-9

USP7-IN-9 是一种高效的 USP7 抑制剂，IC50 为 40.8 nM。USP7-IN-9 能诱导 RS4; 11 细胞凋亡 (apoptosis)，将细胞周期阻滞在 G0/G1 和 S 期。USP7-IN-9 降低癌蛋白 MDM2 和 DNMT1 的水平，升高抑癌蛋白 p53 和 p21 的水平。

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USP7-IN-9 Chemical Structure

USP7-IN-9 Chemical Structure

CAS No. : 2444374-01-8

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规格 价格 是否有货 数量
1 mg ￥1590
In-stock
5 mg ￥3500
In-stock
10 mg ￥6000
In-stock
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100 mg   询价  

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Customer Review

USP7-IN-9 相关抗体

Top Publications Citing Use of Products

查看 Deubiquitinase 亚型特异性产品:

查看所有亚型
USP1 USP2 USP7 USP8 USP30 UCHL1

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

USP7-IN-9 is a highly potent ubiquitin-specific protease 7 (USP7) inhibitor with an IC50 value of 40.8 nM. USP7-IN-9 can induce apoptosis and arrest cell progression at G0/G1 and S phases in RS4; 11 cells. USP7-IN-9 reduces the protein levels of oncoproteins MDM2 and DNMT1 and increases the protein levels of tumor suppressors p53 and p21[1].

IC50 & Target

IC50: 40.8 nM (USP7)[1]
体外研究
(In Vitro)

USP7-IN-9 (compound L55) (0-50 μM; 3 or 6 days) exhibits inhibitory activity against cancer cells[1].
USP7-IN-9 (1 μM; 0-72 hours) reduces the proportions of G2/M cells, and does not change the proportions of G0/G1 and S cells[1].
USP7-IN-9 (0.1-1 μM; 24 hours) reduces the protein levels of MDM2 and DNMT1 in a dose-dependent manner, and increases the protein levels of p53 and p21[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

USP7-IN-9 相关抗体:

Cell Proliferation Assay

Cell Line: LNCaP, RS4; 11, HCT 116, NB4, K562 and HuH-7 cells[1]
Concentration: 0-50 μM
Incubation Time: LNCaP, 6 days; RS4; 11, HCT 116, HuH-7, K562 and NB4, 3 days
Result: Exhibited high inhibitory activity against LNCaP and RS4; 11 cells, with IC50s of 29.6 nM and 41.6 nM, respectively, and weak inhibitory activity on HCT 116, NB4, K562 and HuH-7 cells.

Cell Cycle Analysis

Cell Line: RS4; 11 cells[1]
Concentration: 1 μM
Incubation Time: 0, 24, 48 and 72 hours
Result: Reduced the proportions of G2/M cells, while the proportions of G0/G1 and S cells were not apparently altered.

Western Blot Analysis

Cell Line: RS4; 11 cells[1]
Concentration: 0.1, 0.3 and 1 μM
Incubation Time: 24 hours
Result: Reduced the protein levels of MDM2 and DNMT1 in a dose-dependent manner, and increased the protein levels of p53 and p21.
分子量

779.08

Formula

C32H33ClF6N6O8
CAS 号
性状

固体

颜色

Light yellow to yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (128.36 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2836 mL 6.4178 mL 12.8357 mL
5 mM 0.2567 mL 1.2836 mL 2.5671 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

USP7-IN-9 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2836 mL 6.4178 mL 12.8357 mL 32.0891 mL
5 mM 0.2567 mL 1.2836 mL 2.5671 mL 6.4178 mL
10 mM 0.1284 mL 0.6418 mL 1.2836 mL 3.2089 mL
15 mM 0.0856 mL 0.4279 mL 0.8557 mL 2.1393 mL
20 mM 0.0642 mL 0.3209 mL 0.6418 mL 1.6045 mL
25 mM 0.0513 mL 0.2567 mL 0.5134 mL 1.2836 mL
30 mM 0.0428 mL 0.2139 mL 0.4279 mL 1.0696 mL
40 mM 0.0321 mL 0.1604 mL 0.3209 mL 0.8022 mL
50 mM 0.0257 mL 0.1284 mL 0.2567 mL 0.6418 mL
60 mM 0.0214 mL 0.1070 mL 0.2139 mL 0.5348 mL
80 mM 0.0160 mL 0.0802 mL 0.1604 mL 0.4011 mL
100 mM 0.0128 mL 0.0642 mL 0.1284 mL 0.3209 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

USP7-IN-92444374-01-8DeubiquitinaseApoptosisDUBsUbiquitin-specific protease 7Anti-cancerMDM2DNMT1p53p21RS411 cellsInhibitorinhibitorinhibit

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