1. Cell Cycle/DNA Damage
  2. Nucleoside Antimetabolite/Analog
  3. 2'-C-Methylguanosine

2'-C-Methylguanosine  (Synonyms: 2'-C-beta-Methylguanosine)

目录号: HY-128139
产品使用指南 技术支持

2'-C-Methylguanosine (2'-C-beta-Methylguanosine) 是一种鸟嘌呤核苷类似物。鸟嘌呤核苷类似物具有免疫刺激活性。在一些动物模型中,它们还诱导 I 型干扰素,产生抗病毒作用。研究表明,鸟苷类似物的功能活性依赖于活化 Toll 样受体 7 (TLR7)。

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2'-C-Methylguanosine Chemical Structure

2'-C-Methylguanosine Chemical Structure

CAS No. : 374750-30-8

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

2'-C-Methylguanosine (2'-C-beta-Methylguanosine) is a guanosine analogue. Some guanosine analogs have immunostimulatory activity. In some animal models, they also induce type I interferons, producing antiviral effects. Studies have shown that the functional activity of guanosine analogs is dependent on the activation of Toll-like receptor 7 (TLR7)[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
BHK-21 CC50
> 10 μM
Compound: 2'-MeGuo
Cytotoxicity against BHK21 cells after 48 to 96 hrs by MTT assay
Cytotoxicity against BHK21 cells after 48 to 96 hrs by MTT assay
[PMID: 22047799]
BHK-21 CC50
> 10 μM
Compound: 2'-MeGua
Cytotoxicity against mock-infected BHK21 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against mock-infected BHK21 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 27161176]
BHK-21 CC50
> 10 μM
Compound: 2'-C-methyl-guanosine
Cytotoxicity against BHK21 cells by MTT assay
Cytotoxicity against BHK21 cells by MTT assay
[PMID: 22513121]
BHK-21 EC50
> 100 μM
Compound: Met-Gua
Antiviral activity against YFV 17-D vaccine (Stamaril Pasteur J07B01) infected in BHK21 cells assessed as protection against virus-induced cytopathic effect after 3 or 4 days by MTT assay
Antiviral activity against YFV 17-D vaccine (Stamaril Pasteur J07B01) infected in BHK21 cells assessed as protection against virus-induced cytopathic effect after 3 or 4 days by MTT assay
[PMID: 26479028]
BHK-21 EC50
> 100 μM
Compound: NM 108
Antiviral activity against Yellow fever virus infected in BHK21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
Antiviral activity against Yellow fever virus infected in BHK21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
[PMID: 25082514]
BHK-21 CC50
> 100 μM
Compound: 2'-C-methyl-guanosine
Cytotoxicity against golden hamster BHK21 cells assessed as reduction in cell viability after 48 to 96 hrs by MTT assay
Cytotoxicity against golden hamster BHK21 cells assessed as reduction in cell viability after 48 to 96 hrs by MTT assay
[PMID: 29339251]
BHK-21 CC50
> 100 μM
Compound: Met-Gua
Cytotoxicity against mock-infected hamster BHK21 cells assessed as reduction in proliferation after 72 hrs by MTT method
Cytotoxicity against mock-infected hamster BHK21 cells assessed as reduction in proliferation after 72 hrs by MTT method
[PMID: 25014745]
BHK-21 EC50
0.7 μM
Compound: NM108
Antiviral activity against WNV infected in BHK-21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
Antiviral activity against WNV infected in BHK-21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
[PMID: 26443549]
BHK-21 EC50
1 μM
Compound: NM-108
Antiviral activity against YFV infected in BHK21 cells assessed as protection against virus-induced cytopathogenicity after 4 days by MTT assay
Antiviral activity against YFV infected in BHK21 cells assessed as protection against virus-induced cytopathogenicity after 4 days by MTT assay
[PMID: 18760610]
BHK-21 CC50
1.1 μM
Compound: Met-Gua
Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 26479028]
BHK-21 EC50
1.2 μM
Compound: NM108
Antiviral activity against DENV-2 infected in BHK-21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
Antiviral activity against DENV-2 infected in BHK-21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
[PMID: 26443549]
BHK-21 EC50
1.5 μM
Compound: NM108
Antiviral activity against YFV infected in BHK-21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
Antiviral activity against YFV infected in BHK-21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
[PMID: 26443549]
BHK-21 EC50
1.8 μM
Compound: NM 108
Antiviral activity against Yellow fever virus infected in BHK-21 cells assessed as inhibition of virus-induced cytopathicity after 3 to 4 days by MTT assay
Antiviral activity against Yellow fever virus infected in BHK-21 cells assessed as inhibition of virus-induced cytopathicity after 3 to 4 days by MTT assay
[PMID: 19482481]
BHK-21 EC50
1.9 μM
Compound: Met-Gua
Antiviral activity against Reovirus 1 ATCC VR- 214 infected in BHK21 cells assessed as protection against virus-induced cytopathic effect after 3 or 4 days by MTT assay
Antiviral activity against Reovirus 1 ATCC VR- 214 infected in BHK21 cells assessed as protection against virus-induced cytopathic effect after 3 or 4 days by MTT assay
[PMID: 26479028]
BHK-21 EC50
1.9 μM
Compound: 2'-MeGua
Antiviral activity against Yellow fever virus 17D infected in BHK21 cells assessed as inhibition of virus induced cytopathogenicity after 3 to 4 days by MTT assay
Antiviral activity against Yellow fever virus 17D infected in BHK21 cells assessed as inhibition of virus induced cytopathogenicity after 3 to 4 days by MTT assay
[PMID: 27161176]
BHK-21 EC50
1.9 μM
Compound: Met-Gua
Antiviral activity against Yellow fever virus 17D Stamaril Pasteur J07B01 infected in BHK21 cells assessed as protection from virus-induced cytopathogenicity after 3 to 4 days by MTT method
Antiviral activity against Yellow fever virus 17D Stamaril Pasteur J07B01 infected in BHK21 cells assessed as protection from virus-induced cytopathogenicity after 3 to 4 days by MTT method
[PMID: 25014745]
BHK-21 EC50
1.9 μM
Compound: 2'-C-methyl-guanosine
Antiviral activity against Yellow fever virus 17D infected in BHK21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
Antiviral activity against Yellow fever virus 17D infected in BHK21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
[PMID: 22513121]
BHK-21 EC50
1.9 μM
Compound: 2'-MeGuo
Antiviral activity against Yellow fever virus 17D Stamaril Pasteur J07B01 infected in hamster BHK21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
Antiviral activity against Yellow fever virus 17D Stamaril Pasteur J07B01 infected in hamster BHK21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
[PMID: 22047799]
BHK-21 EC50
2.4 μM
Compound: NM108
Antiviral activity against Reo-1 virus infected in BHK-21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
Antiviral activity against Reo-1 virus infected in BHK-21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
[PMID: 26443549]
BHK-21 EC50
2.4 μM
Compound: NM 108
Antiviral activity against Reovirus type-1 infected in BHK21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
Antiviral activity against Reovirus type-1 infected in BHK21 cells assessed as inhibition of virus-induced cytopathogenicity after 3 to 4 days by MTT assay
[PMID: 25082514]
BHK-21 EC50
2.4 μM
Compound: NM 108
Antiviral activity against Reovirus 1 3651 infected in BHK21 cells assessed as protection from virus-induced cytopathogenicity after 3 to 4 days by MTT method
Antiviral activity against Reovirus 1 3651 infected in BHK21 cells assessed as protection from virus-induced cytopathogenicity after 3 to 4 days by MTT method
[PMID: 20359898]
BHK-21 EC50
2.4 μM
Compound: NM 108
Antiviral activity against Reovirus-1 infected in BHK-21 cells assessed as inhibition of virus-induced cytopathicity after 3 to 4 days by MTT assay
Antiviral activity against Reovirus-1 infected in BHK-21 cells assessed as inhibition of virus-induced cytopathicity after 3 to 4 days by MTT assay
[PMID: 19482481]
BHK-21 EC50
2.5 μM
Compound: NM108
Antiviral activity against Yellow fever virus infected Syrian hamster BHK-21 cells assessed as protection against virus-induced cytopathogenicity treated 1 hr after viral infection measured after 3-4 days by MTT method
Antiviral activity against Yellow fever virus infected Syrian hamster BHK-21 cells assessed as protection against virus-induced cytopathogenicity treated 1 hr after viral infection measured after 3-4 days by MTT method
10.1007/s00044-012-0342-1
BHK-21 CC50
90 μM
Compound: NM 108
Cytotoxicity against mock-infected BHK21 cells after 48 to 96 hrs by MTT assay
Cytotoxicity against mock-infected BHK21 cells after 48 to 96 hrs by MTT assay
[PMID: 25082514]
BHK-21 CC50
90 μM
Compound: NM 108
Cytotoxicity against hamster BHK21 cells assessed as reduction in cell viability after 48 to 96 hrs by MTT assay
Cytotoxicity against hamster BHK21 cells assessed as reduction in cell viability after 48 to 96 hrs by MTT assay
[PMID: 19482481]
Huh-7 CC50
> 100 μM
Compound: 1c
Cytotoxicity against human HuH7 cells after 72 hrs by celltiter-glo assay
Cytotoxicity against human HuH7 cells after 72 hrs by celltiter-glo assay
[PMID: 20527890]
Huh-7 CC50
> 100 μM
Compound: 1
Cytotoxicity against Huh7 cells by MTS assay
Cytotoxicity against Huh7 cells by MTS assay
[PMID: 16368235]
Huh-7 CC50
> 100 μM
Compound: 4
Cytotoxicity against human HuH7 cells after 3 days
Cytotoxicity against human HuH7 cells after 3 days
[PMID: 22039920]
Huh-7 CC50
> 89 μM
Compound: 2'-C-Me G
Cytotoxicity against human HuH7 cells after 24 hrs by MTS assay
Cytotoxicity against human HuH7 cells after 24 hrs by MTS assay
[PMID: 22578461]
Huh-7 EC50
2.2 μM
Compound: 4
Antiviral activity against Hepatitis C virus subtype 1b Con1 infected in human Huh7 cells assessed as inhibition of HCV replicon-encoded Renilla luciferase reporter activity after 72 hrs by HCV replicon assay
Antiviral activity against Hepatitis C virus subtype 1b Con1 infected in human Huh7 cells assessed as inhibition of HCV replicon-encoded Renilla luciferase reporter activity after 72 hrs by HCV replicon assay
[PMID: 22039920]
Huh-7 EC50
2.3 μM
Compound: 8, 2'-C-MeG
Antiviral activity against HCV in human Huh7 cells by RNA replicon assay
Antiviral activity against HCV in human Huh7 cells by RNA replicon assay
[PMID: 19433343]
Huh-7 EC50
3.25 μM
Compound: 2'-C-Me G
Antiviral activity against HCV genotype 1b infected in human HuH7 cells assessed as cytoprotection after 24 hrs
Antiviral activity against HCV genotype 1b infected in human HuH7 cells assessed as cytoprotection after 24 hrs
[PMID: 22578461]
Huh-7 EC50
3.5 μM
Compound: 1c
Antiviral activity against HCV1b infected in human HuH7 cells assessed as inhibition of viral replication after 48 hrs by luciferase reporter gene assay
Antiviral activity against HCV1b infected in human HuH7 cells assessed as inhibition of viral replication after 48 hrs by luciferase reporter gene assay
[PMID: 20527890]
MDCK EC50
1.7 μM
Compound: NM-108
Antiviral activity against BVDV infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity by MTT assay
Antiviral activity against BVDV infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity by MTT assay
[PMID: 20638852]
MT4 CC50
> 100 μM
Compound: NM108
Cytotoxicity against human MT4 cells assessed as cell viability after 96 hrs by MTT assay
Cytotoxicity against human MT4 cells assessed as cell viability after 96 hrs by MTT assay
[PMID: 26443549]
MT4 CC50
> 100 μM
Compound: NM 108
Cytotoxicity against mock-infected human MT4 cells after 96 hrs by MTT assay
Cytotoxicity against mock-infected human MT4 cells after 96 hrs by MTT assay
[PMID: 25082514]
MT4 CC50
> 100 μM
Compound: NM 108
Cytotoxicity against human MT4 cells after 96 hrs by MTT assay
Cytotoxicity against human MT4 cells after 96 hrs by MTT assay
[PMID: 20359898]
MT4 CC50
> 100 μM
Compound: NM 108
Cytotoxicity against human MT4 cells after 96 hrs by MTT assay
Cytotoxicity against human MT4 cells after 96 hrs by MTT assay
[PMID: 19482481]
MT4 CC50
> 100 μM
Compound: NM-108
Cytotoxicity against mock-infected human MT4 cells assessed as reduction in cell viability after 96 hrs by MTT assay
Cytotoxicity against mock-infected human MT4 cells assessed as reduction in cell viability after 96 hrs by MTT assay
[PMID: 18760610]
MT4 CC50
> 100 μM
Compound: NM 108
Cytotoxicity against human MT4 cells after 96 hrs by MTT method
Cytotoxicity against human MT4 cells after 96 hrs by MTT method
[PMID: 18054491]
Vero CC50
> 100 μM
Compound: NM 108
Cytotoxicity against african green monkey Vero 76 cells by crystal violet staining
Cytotoxicity against african green monkey Vero 76 cells by crystal violet staining
[PMID: 19482481]
Vero CC50
> 100 μM
Compound: NM-108
Cytotoxicity against mock-infected african green monkey Vero76 cells assessed as reduction in cell viability after 96 hrs by MTT assay
Cytotoxicity against mock-infected african green monkey Vero76 cells assessed as reduction in cell viability after 96 hrs by MTT assay
[PMID: 18760610]
Vero EC50
> 100 μM
Compound: NM 108
Antiviral activity against Poliovirus type 1 Sabin strain infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 2 days by plaque reduction assay
Antiviral activity against Poliovirus type 1 Sabin strain infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 2 days by plaque reduction assay
[PMID: 25082514]
Vero EC50
> 100 μM
Compound: NM 108
Antiviral activity against Vesicular stomatitis virus infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 2 days by plaque reduction assay
Antiviral activity against Vesicular stomatitis virus infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 2 days by plaque reduction assay
[PMID: 25082514]
Vero EC50
> 100 μM
Compound: NM 108
Antiviral activity against Polio virus type1 Sabin strain Chat infected in african green monkey Vero cells after 2 days by plaque reduction assay
Antiviral activity against Polio virus type1 Sabin strain Chat infected in african green monkey Vero cells after 2 days by plaque reduction assay
[PMID: 20359898]
Vero EC50
> 100 μM
Compound: NM 108
Antiviral activity against Vaccinia Virus infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 3 days by plaque reduction assay
Antiviral activity against Vaccinia Virus infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 3 days by plaque reduction assay
[PMID: 25082514]
Vero EC50
> 100 μM
Compound: NM 108
Antiviral activity against HSV1 infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 3 days by plaque reduction assay
Antiviral activity against HSV1 infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 3 days by plaque reduction assay
[PMID: 25082514]
Vero EC50
> 100 μM
Compound: NM 108
Antiviral activity against HSV1 KOS infected in african green monkey Vero cells after 3 days by plaque reduction assay
Antiviral activity against HSV1 KOS infected in african green monkey Vero cells after 3 days by plaque reduction assay
[PMID: 20359898]
Vero EC50
> 100 μM
Compound: NM 108
Antiviral activity against Vaccinia virus Elstree-Lister infected in african green monkey Vero cells after 3 days by plaque reduction assay
Antiviral activity against Vaccinia virus Elstree-Lister infected in african green monkey Vero cells after 3 days by plaque reduction assay
[PMID: 20359898]
Vero EC50
> 100 μM
Compound: NM 108
Antiviral activity against Respiratory syncytial virus infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 5 days by plaque reduction assay
Antiviral activity against Respiratory syncytial virus infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 5 days by plaque reduction assay
[PMID: 25082514]
Vero EC50
> 100 μM
Compound: NM 108
Antiviral activity against RSV A2 infected in african green monkey Vero cells after 5 days by plaque reduction assay
Antiviral activity against RSV A2 infected in african green monkey Vero cells after 5 days by plaque reduction assay
[PMID: 20359898]
Vero CC50
> 200 μM
Compound: 1; 2'-C-beta-Me-G
Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability after 72 hrs by neutral red dye uptake assay
Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability after 72 hrs by neutral red dye uptake assay
[PMID: 28947944]
Vero EC50
20 μM
Compound: NM 108
Antiviral activity against Coxsackievirus B5 infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 3 days by plaque reduction assay
Antiviral activity against Coxsackievirus B5 infected in african green monkey Vero 76 cells assessed as reduction in plaque number after 3 days by plaque reduction assay
[PMID: 25082514]
Vero EC50
20 μM
Compound: NM 108
Antiviral activity against Human coxsackievirus B2 Ohio-1 infected in african green monkey Vero cells after 3 days by plaque reduction assay
Antiviral activity against Human coxsackievirus B2 Ohio-1 infected in african green monkey Vero cells after 3 days by plaque reduction assay
[PMID: 20359898]
Vero 76 EC50
> 100 μM
Compound: NM-108
Antiviral activity against RSV infected in Vero76 cells assessed as reduction in virus plaque formation after 4 days by MTT assay
Antiviral activity against RSV infected in Vero76 cells assessed as reduction in virus plaque formation after 4 days by MTT assay
[PMID: 18760610]
Vero 76 EC50
> 100 μM
Compound: NM-108
Antiviral activity against Sb1 virus infected in Vero76 cells assessed as reduction in virus plaque formation after 4 days by MTT assay
Antiviral activity against Sb1 virus infected in Vero76 cells assessed as reduction in virus plaque formation after 4 days by MTT assay
[PMID: 18760610]
Vero 76 CC50
> 100 μM
Compound: NM 108
Cytotoxicity against mock-infected african green monkey Vero 76 cells after 48 to 96 hrs by MTT assay
Cytotoxicity against mock-infected african green monkey Vero 76 cells after 48 to 96 hrs by MTT assay
[PMID: 25082514]
Vero 76 EC50
> 100 μM
Compound: NM 108
Antiviral activity against HSV1 infected in Vero-76 cells after 3 days by plaque reduction assay
Antiviral activity against HSV1 infected in Vero-76 cells after 3 days by plaque reduction assay
[PMID: 19482481]
Vero 76 CC50
> 100 μM
Compound: NM108
Cytotoxicity against african green monkey Vero 76 cells assessed as cell viability after 48 to 96 hrs by crystal violet staining method
Cytotoxicity against african green monkey Vero 76 cells assessed as cell viability after 48 to 96 hrs by crystal violet staining method
[PMID: 26443549]
Vero 76 EC50
> 100 μM
Compound: NM108
Antiviral activity against human RSV infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity after 5 days by plaque reduction assay
Antiviral activity against human RSV infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity after 5 days by plaque reduction assay
[PMID: 26443549]
Vero 76 EC50
> 100 μM
Compound: NM 108
Antiviral activity against Respiratory syncytial virus A2 infected in Vero-76 cells after 5 days by plaque reduction assay
Antiviral activity against Respiratory syncytial virus A2 infected in Vero-76 cells after 5 days by plaque reduction assay
[PMID: 19482481]
Vero 76 EC50
> 100 μM
Compound: NM108
Antiviral activity against Human poliovirus 1 Sabin infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity by plaque reduction assay
Antiviral activity against Human poliovirus 1 Sabin infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity by plaque reduction assay
[PMID: 26443549]
Vero 76 EC50
20 μM
Compound: NM-108
Antiviral activity against CVB2 infected in Vero76 cells assessed as reduction in virus plaque formation after 4 days by MTT assay
Antiviral activity against CVB2 infected in Vero76 cells assessed as reduction in virus plaque formation after 4 days by MTT assay
[PMID: 18760610]
Vero 76 EC50
20 μM
Compound: NM108
Antiviral activity against human CVB5 infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity after 3 days by plaque reduction assay
Antiviral activity against human CVB5 infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity after 3 days by plaque reduction assay
[PMID: 26443549]
Vero 76 EC50
20 μM
Compound: NM 108
Antiviral activity against Coxsackie virus type B2 virus infected in african green monkey Vero-76 cells after 3 days by plaque reduction assay
Antiviral activity against Coxsackie virus type B2 virus infected in african green monkey Vero-76 cells after 3 days by plaque reduction assay
[PMID: 19482481]
分子量

297.27

Formula

C11H15N5O5

CAS 号
中文名称

2-氨基-9-((2R,3R,4R,5R)-3,4-二羟基-5-(羟甲基)-3-甲基四氢呋喃-2-基)-1H-嘌呤-6(9H)-酮

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
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  • Do most proteins show cross-species activity?

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产品名称:
2'-C-Methylguanosine
目录号:
HY-128139
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