2. Vitamin D Related/Nuclear Receptor Autophagy
  3. Androgen Receptor Autophagy
  4. 3,3'-Diindolylmethane

3,3'-Diindolylmethane  (Synonyms: DIM; Arundine; HB 236)

目录号: HY-15758 纯度: 99.93%
COA 产品使用指南

摩尔计算器

3，3'-Diindolylmethane 是一种有效的雄激素受体 (AR) 拮抗剂。

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3,3'-Diindolylmethane Chemical Structure

3,3'-Diindolylmethane Chemical Structure

CAS No. : 1968-05-4

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥500
In-stock
100 mg ￥450
In-stock
200 mg ￥550
In-stock
500 mg ￥650
In-stock
1 g   询价  
5 g   询价  

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Customer Review

Other Forms of 3,3'-Diindolylmethane:

3,3'-Diindolylmethane 相关抗体

Top Publications Citing Use of Products

    3,3'-Diindolylmethane purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2020 Jun 16;39(1):113.  [Abstract]

    IHC results of xenografts staining shows downexpression of AHR, RhoA, ROCK1, COX2 and mesenchymal cell markers as well as upregulation of epithelial cell marker Claudin-1.

    3,3'-Diindolylmethane purchased from MCE. Usage Cited in: J Cell Commun Signal. 2020 Jun;14(2):175-192.  [Abstract]

    Western blot shows the results that at the 48 h time point, cells exerted the potent reactivity when exposing DIM treatment in that the expression of AHR reduces gradually on the basis of increased DIM concentrations and the production of CYP1A1 increases in the same way. ​

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    3,3'-Diindolylmethane is a strong, pure androgen receptor (AR) antagonist.

    IC50 & Target

    Androgen receptor[1]
    细胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    CHO EC50
    0.368 μM
    Compound: 8; DIM
    Positive allosteric modulation of recombinant human GPR84 expressed in CHO cell membranes co-expressing beta-arrestin2 assessed as increase in [3H]PSB-1584 binding measured after 6 hrs by scintillation counting method
    Positive allosteric modulation of recombinant human GPR84 expressed in CHO cell membranes co-expressing beta-arrestin2 assessed as increase in [3H]PSB-1584 binding measured after 6 hrs by scintillation counting method
    		[PMID: 31721581]
    CHO EC50
    0.5 μM
    Compound: 8; DIM
    Agonist activity at human N-terminal FLAG-tagged GPR84 receptor stably expressed in CHO cell membranes assessed as stimulation of [35S]GTPgammaS binding incubated for 1 hr by liquid scintillation counting method
    Agonist activity at human N-terminal FLAG-tagged GPR84 receptor stably expressed in CHO cell membranes assessed as stimulation of [35S]GTPgammaS binding incubated for 1 hr by liquid scintillation counting method
    		[PMID: 31721581]
    CHO EC50
    0.7 μM
    Compound: 8; DIM
    Agonist activity at human N-terminal FLAG-tagged GPR84 receptor stably expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production preincubated for 20 mins followed by forskolin addition and measured after 20 mins
    Agonist activity at human N-terminal FLAG-tagged GPR84 receptor stably expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production preincubated for 20 mins followed by forskolin addition and measured after 20 mins
    		[PMID: 31721581]
    HEK293 EC50
    1.11 μM
    Compound: 8; DIM
    Agonist activity at recombinant human HA-tagged GPR84 stably expressed in HEK293 cells assessed as stimulation of [35S]GTPgammaS binding measured after 30 mins by liquid scintillation counting method
    Agonist activity at recombinant human HA-tagged GPR84 stably expressed in HEK293 cells assessed as stimulation of [35S]GTPgammaS binding measured after 30 mins by liquid scintillation counting method
    		[PMID: 31721581]
    MCF7 IC50
    50 μM
    Compound: DIM
    Growth inhibition of human MCF7 cells after 48 hrs by MTT assay
    Growth inhibition of human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 22277074]
    MDA-MB-231 IC50
    10 μM
    Compound: DIM
    Growth inhibition of human MDA-MB-231 cells after 48 hrs by MTT assay
    Growth inhibition of human MDA-MB-231 cells after 48 hrs by MTT assay
    		[PMID: 22277074]
    Sf9 EC50
    11 μM
    Compound: 8; DIM
    Agonist activity at human Gi1alpha-coupled GPR84 expressed in baculovirus infected Sf9 insect cells assessed as stimulation of [35S]GTPgammaS binding measured after 30 mins by top-count liquid scintillation counting method
    Agonist activity at human Gi1alpha-coupled GPR84 expressed in baculovirus infected Sf9 insect cells assessed as stimulation of [35S]GTPgammaS binding measured after 30 mins by top-count liquid scintillation counting method
    		[PMID: 31721581]
    Sf9 EC50
    5.9 μM
    Compound: 8; DIM
    Agonist activity at human Gi1alpha-coupled GPR84 expressed in baculovirus infected Sf9 insect cells assessed as stimulation of [35S]GTPgammaS binding measured after 30 mins by liquid scintillation counting method
    Agonist activity at human Gi1alpha-coupled GPR84 expressed in baculovirus infected Sf9 insect cells assessed as stimulation of [35S]GTPgammaS binding measured after 30 mins by liquid scintillation counting method
    		[PMID: 31721581]
    U-251 EC50
    280 μM
    Compound: 6; DIM
    Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay
    Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay
    		[PMID: 28256372]
    体外研究
    (In Vitro)

    3,3'-Diindolylmethane (DIM) 抑制 LNCaP 细胞的细胞增殖并抑制 DHT 对 DNA 合成的刺激[1]。
    3,3'-Diindolylmethane 抑制内源性 PSA 转录并降低 LNCaP 细胞中由 DHT 诱导的细胞内和分泌的 PSA 蛋白水平[1]。
    3,3'-Diindolylmethane 以浓度依赖性方式抑制瞬时转染的 LNCaP 细胞中 DHT 诱导的前列腺特异性抗原启动子调节报告基因构建体的表达。与 50 μM 3,3'-Diindolylmethane 共同处理可部分抑制 DHT 处理诱导的 AR 易位，并显示 AR 在细胞质和细胞核中的分布[1]。
    3,3'-Diindolylmethane 处理可防止在细胞核中形成 AR 病灶[1]。
    3,3'-Diindolylmethane 单独产生主要分布在细胞质中的荧光[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    3,3'-Diindolylmethane 相关抗体:
    体内研究
    (In Vivo)

    3,3'-Diindolylmethane (DIM) (10 mg/kg; s.c.; everyday)，持续 30 天。可显著抑制 SNU-484 异种移植肿瘤的生长。在药物暴露 30 天后，两组小鼠的体重与载体对照组没有显著差异，这表明 3,3'-Diindolylmethane 对小鼠没有严重毒性。3,3'-Diindolylmethane 给药显著抑制了由 YAP 失活介导的 SNU-484 异种移植物体内生长[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    246.31

    Formula

    C17H14N2
    CAS 号
    性状

    固体

    颜色

    Off-white to pink

    中文名称

    3,3'-二吲哚甲烷
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : ≥ 100 mg/mL (405.99 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 4.0599 mL 20.2994 mL 40.5988 mL
    5 mM 0.8120 mL 4.0599 mL 8.1198 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.15 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (10.15 mM); 悬浊液; 超声助溶

      此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.93%

    COA (291 KB) HNMR (123 KB) RP-HPLC (97 KB) GC (180 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [1]

    The human prostate adenocarcinoma cell lines LNCaP-FGC and PC-3 are grown as adherent monolayers in 10% FBS-DMEM, supplemented with 4.0 g/L glucose and 3.7 g/L sodium bicarbonate in a humidified incubator at 37°C and 5% CO2, and passaged at ~80% confluency. Cultures used in subsequent experiments are at less than 40 passages. Cells grown in stripped conditions are in 5% DCC-FBS-DMEM base supplemented with 4 g/L glucose, 3.7 g/L sodium bicarbonate, and 0.293 g/L L-glutamine. Before the beginning of the treatments, cells are depleted of androgen for 4-7 days in medium composed of DMEM base without phenol red and with 4 g/L glucose and 3.7 g/L sodium bicarbonate. During the depletion period, medium is changed every 48 h. Treatments are administered by the addition of 1 μL of a 1,000-fold concentrated solution of 3,3'-Diindolylmethane in Me2SO/mL of medium. Once the treatment period started, medium is changed daily to counter possible loss of readily metabolized compounds[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Mice[2]
    Four-week-old female SPF/VAF immunodeficient mice are injected subcutaneously (s.c.) into the right flank with 0.1 mL Matrigel containing 3.5×106 human gastric cancer cells (SNU-484). The mice are randomized into 2 groups 1 week after tumor implantation: i) the untreated control group (n=5, DMSO in 50 μL PBS daily) and ii) the 3,3'-Diindolylmethane-treated group (n=5, 10 mg/kg in 50 μL PBS once daily). Gastric primary tumors are excised, and the final tumor volume is measured once every 3 days using a caliper and calculated as (width)2×length/2. The experiment is terminated on day 39. Half of the tumor tissue is prepared for western blotting and the other half is snap frozen in liquid nitrogen and stored at −80°C.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    3,3'-Diindolylmethane 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.0599 mL 20.2994 mL 40.5987 mL 101.4969 mL
    5 mM 0.8120 mL 4.0599 mL 8.1197 mL 20.2994 mL
    10 mM 0.4060 mL 2.0299 mL 4.0599 mL 10.1497 mL
    15 mM 0.2707 mL 1.3533 mL 2.7066 mL 6.7665 mL
    20 mM 0.2030 mL 1.0150 mL 2.0299 mL 5.0748 mL
    25 mM 0.1624 mL 0.8120 mL 1.6239 mL 4.0599 mL
    30 mM 0.1353 mL 0.6766 mL 1.3533 mL 3.3832 mL
    40 mM 0.1015 mL 0.5075 mL 1.0150 mL 2.5374 mL
    50 mM 0.0812 mL 0.4060 mL 0.8120 mL 2.0299 mL
    60 mM 0.0677 mL 0.3383 mL 0.6766 mL 1.6916 mL
    80 mM 0.0507 mL 0.2537 mL 0.5075 mL 1.2687 mL
    100 mM 0.0406 mL 0.2030 mL 0.4060 mL 1.0150 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    3,3'-Diindolylmethane1968-05-4DIM Arundine HB 2363,3'-二吲哚甲烷HB236HB 236HB-236Androgen ReceptorAutophagyInhibitorinhibitorinhibit

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