2. Metabolic Enzyme/Protease Anti-infection
  3. Glutaminase Bacterial Influenza Virus Antibiotic
  4. 6-Diazo-5-oxo-L-nor-Leucine

6-Diazo-5-oxo-L-nor-Leucine  (Synonyms: 6-重氮-5-氧代-L-正亮氨酸; L-6-Diazo-5-oxonorleucine; DON)

目录号: HY-108357 纯度: 99.98%
COA 产品使用指南

摩尔计算器

6-Diazo-5-oxo-L-nor-Leucine (L-6-Diazo-5-oxonorleucine; DON) 是一种谷氨酰胺拮抗剂，能不可逆地抑制谷氨酰胺的分解作用。6-Diazo-5-oxo-L-nor-Leucine 具有较好的抗癌活性 (尤其是胰腺癌)，能降低肿瘤细胞的自我更新潜力和转移能力。6-Diazo-5-oxo-L-nor-Leucine 还具有抗菌和抗病毒活性。

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6-Diazo-5-oxo-L-nor-Leucine Chemical Structure

6-Diazo-5-oxo-L-nor-Leucine Chemical Structure

CAS No. : 157-03-9

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥1713
In-stock
1 mg ￥910
In-stock
5 mg ￥3850
In-stock
10 mg ￥5968
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

6-Diazo-5-oxo-L-nor-Leucine 相关抗体

Top Publications Citing Use of Products

    6-Diazo-5-oxo-L-nor-Leucine purchased from MCE. Usage Cited in: Front Immunol. 2022 May 19;13:880262.  [Abstract]

    Splenocytes treated with 6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) has lower expressions of CD25 and CD69 in CD4(+) T cells.

    6-Diazo-5-oxo-L-nor-Leucine purchased from MCE. Usage Cited in: Front Immunol. 2022 May 19;13:880262.  [Abstract]

    6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) inhibits the the levels of phosphorylated mTOR and P70S6K (ConA-induced) in splenocytes.

    6-Diazo-5-oxo-L-nor-Leucine purchased from MCE. Usage Cited in: Front Immunol. 2022 May 19;13:880262.  [Abstract]

    Splenocytes treated with 6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) has lower expressions of CD25 and CD69 in CD8(+) T cells.

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    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    6-Diazo-5-oxo-L-nor-Leucine (L-6-Diazo-5-oxonorleucine; DON) is a glutamine antagonist that irreversibly inhibits the catabolic effect of glutamine. 6-Diazo-5-oxo-L-nor-Leucine shows good anticancer activity (especially in pancreatic cancer) and reduces the self-renewal potential and metastatic capacity of tumour cells. 6-Diazo-5-oxo-L-nor-Leucine also possesses antibacterial and antiviral activity[1][2][3].

    IC50 & Target

    Bacterial[3].
    体外研究
    (In Vitro)

    6-Diazo-5-oxo-L-nor-Leucine (DON) (0.3 mM; 1 h) shows inhibition of glutamine catabolism in WI-L2 cells[1].
    ? 6-Diazo-5-oxo-L-nor-Leucine (DON) decreases the selfrenewal potential and metastatic ability of tumor cell[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    6-Diazo-5-oxo-L-nor-Leucine 相关抗体:

    Cell Viability Assay[1]

    Cell Line: WI-L2 cells
    Concentration: 0.3 mM
    Incubation Time: 1 h
    Result: Inhibited the rapid catabolism of glutamine by the cultured human lymphoblast line WI-L2 greater than 95%.
    体内研究
    (In Vivo)

    6-Diazo-5-oxo-L-nor-Leucine sensitizes pancreatic tumors to anti-PD1 resulting in tumor regression and prolonged survival in vivo[2].
    ? 6-Diazo-5-oxo-L-nor-Leucine decreases hyaluronan and collagen in the tumor microenvironment, leading to an extensive remodeling of the ECM (extensive extracellular matrix), and an increases infiltration CD8+ T-cells[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    171.15

    Formula

    C6H9N3O3
    CAS 号
    性状

    固体

    颜色

    Off-white to light yellow

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 25 mg/mL (146.07 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 5.8428 mL 29.2141 mL 58.4283 mL
    5 mM 1.1686 mL 5.8428 mL 11.6857 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.98%

    COA (282 KB) HNMR (243 KB) RP-HPLC (259 KB) MS (68 KB) OR (293 KB) 元素分析报告 (206 KB)

    产品使用指南 (1538 KB)
    参考文献

    6-Diazo-5-oxo-L-nor-Leucine 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.8428 mL 29.2141 mL 58.4283 mL 146.0707 mL
    5 mM 1.1686 mL 5.8428 mL 11.6857 mL 29.2141 mL
    10 mM 0.5843 mL 2.9214 mL 5.8428 mL 14.6071 mL
    15 mM 0.3895 mL 1.9476 mL 3.8952 mL 9.7380 mL
    20 mM 0.2921 mL 1.4607 mL 2.9214 mL 7.3035 mL
    25 mM 0.2337 mL 1.1686 mL 2.3371 mL 5.8428 mL
    30 mM 0.1948 mL 0.9738 mL 1.9476 mL 4.8690 mL
    40 mM 0.1461 mL 0.7304 mL 1.4607 mL 3.6518 mL
    50 mM 0.1169 mL 0.5843 mL 1.1686 mL 2.9214 mL
    60 mM 0.0974 mL 0.4869 mL 0.9738 mL 2.4345 mL
    80 mM 0.0730 mL 0.3652 mL 0.7304 mL 1.8259 mL
    100 mM 0.0584 mL 0.2921 mL 0.5843 mL 1.4607 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    6-Diazo-5-oxo-L-nor-Leucine157-03-9L-6-Diazo-5-oxonorleucine DONGlutaminaseBacterialInfluenza VirusAntibioticpancreatic cancercancerantitumourWI-L2CD8+ T-cellsanti-PD1bacterialvirusInhibitorinhibitorinhibit

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    目录号：
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