2. Metabolic Enzyme/Protease Autophagy
  3. HIF/HIF Prolyl-Hydroxylase Autophagy
  4. DMOG

DMOG  (Synonyms: Dimethyloxallyl Glycine)

目录号: HY-15893 纯度: 99.69%
COA 产品使用指南

摩尔计算器

DMOG (Dimethyloxallyl Glycine) 是具有细胞渗透和竞争性 HIF-PH 抑制剂，可导致蛋白 HIF-1α 的积聚和稳定。DMOG 是 α-ketoglutarate 的类似物并且可以抑制 α-KG 依耐性羟化酶。DMOG 是一种促血管生成剂，在结肠炎和腹泻动物模型中起保护作用。DMOG 可以诱导细胞自噬 (autophagy).

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DMOG Chemical Structure

DMOG Chemical Structure

CAS No. : 89464-63-1

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥715
In-stock
50 mg ￥650
In-stock
100 mg ￥990
In-stock
200 mg ￥1750
In-stock
500 mg ￥3950
In-stock
1 g   询价  
5 g   询价  

* Please select Quantity before adding items.

Customer Review

DMOG 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 51 篇科研文献

WB

    DMOG purchased from MCE. Usage Cited in: Front Immunol. 2018 Jul 23;9:1667.  [Abstract]

    Manipulation of the protein level of hypoxia-inducible factor 1 alpha (HIF-1α) in NR8383. 50 µM PX478 treatment for 20 h is used to downregulate the HIF-1α protein level in NR8383 cells (A). 1 mM DMOG treatment for 8 h is used to upregulate the HIF-1α protein level in NR8383 cells (B).

    DMOG purchased from MCE. Usage Cited in: J Biol Chem. 2015 Jan 16;290(3):1389-94.  [Abstract]

    The hypoxia induced the expression of NEK8. Cells are treated with CoCl2 (100 μM) or DMOG (100 μM) for 6 h or cultured for 12 h in hypoxia (1.0% oxygen). HIF-1α and NEK8 are analyzed by immunoblotting, GAPDH is employed as a loading control.

    查看 HIF/HIF Prolyl-Hydroxylase 亚型特异性产品:

    查看所有亚型
    HIF-1α HIF

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation in vitro and in vivo[1]. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal[2][4]. DMOG induces cell autophagy[5].

    IC50 & Target

    HIF-1α prolyl hydroxylase[1]
    体外研究
    (In Vitro)

    DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1]. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    DMOG 相关抗体:
    体内研究
    (In Vivo)

    DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice[2]. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    175.14

    Formula

    C6H9NO5
    CAS 号
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 100 mg/mL (570.97 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : 50 mg/mL (285.49 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 5.7097 mL 28.5486 mL 57.0972 mL
    5 mM 1.1419 mL 5.7097 mL 11.4194 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 150 mg/mL (856.46 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.69%

    COA (281 KB) HNMR (132 KB) RP-HPLC (247 KB) LCMS (104 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [3]

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Two groups of mice (C57Bl6) are used. One group (n=11) receives dimethyloxalylglycine (DMOG) i.p. (8 mg in 0.5 mL saline) on days 1, 3, 5, 7 and 9 while the animals in the other group are injected with sterile saline (0.5 mL) at the same intervals (n=6). A third group is treated with DMOG without ligation (n=4) and four unoperated mice serve as controls. After 11 days mice are terminally anaesthetized and the extensor digitorum longus (EDL) and tibialis anterior (TA) muscles excised.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    DMOG 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.7097 mL 28.5486 mL 57.0972 mL 142.7429 mL
    5 mM 1.1419 mL 5.7097 mL 11.4194 mL 28.5486 mL
    10 mM 0.5710 mL 2.8549 mL 5.7097 mL 14.2743 mL
    15 mM 0.3806 mL 1.9032 mL 3.8065 mL 9.5162 mL
    20 mM 0.2855 mL 1.4274 mL 2.8549 mL 7.1371 mL
    25 mM 0.2284 mL 1.1419 mL 2.2839 mL 5.7097 mL
    30 mM 0.1903 mL 0.9516 mL 1.9032 mL 4.7581 mL
    40 mM 0.1427 mL 0.7137 mL 1.4274 mL 3.5686 mL
    50 mM 0.1142 mL 0.5710 mL 1.1419 mL 2.8549 mL
    60 mM 0.0952 mL 0.4758 mL 0.9516 mL 2.3790 mL
    80 mM 0.0714 mL 0.3569 mL 0.7137 mL 1.7843 mL
    100 mM 0.0571 mL 0.2855 mL 0.5710 mL 1.4274 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DMOG89464-63-1Dimethyloxallyl GlycineHIF/HIF Prolyl-HydroxylaseAutophagyHypoxia-inducible factorsHIFsHIF-PH diarrhoeastabilisationaccmulationpro-angiogenicInhibitorinhibitorinhibit

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