2. Metabolic Enzyme/Protease
  3. HIF/HIF Prolyl-Hydroxylase
  4. Dencichine

Dencichine  (Synonyms: 三七素; Dencichin; ODAP)

目录号: HY-N1477 纯度: 98.93%
COA 产品使用指南

摩尔计算器

Dencichin 是从三七中分到的非蛋白氨基酸，能够抑制 HIF-prolyl hydroxylase-2 (PHD-2) 的活性。

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Dencichine Chemical Structure

Dencichine Chemical Structure

CAS No. : 5302-45-4

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1820
In-stock
5 mg ￥1650
In-stock
10 mg ￥2650
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Dencichine:

Dencichine 相关抗体

Top Publications Citing Use of Products

查看 HIF/HIF Prolyl-Hydroxylase 亚型特异性产品:

查看所有亚型
HIF-1α HIF

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Dencichin is a non-protein amino acid originally extracted from Panax notoginseng, and can inhibit HIF-prolyl hydroxylase-2 (PHD-2) activity.

IC50 & Target

PHD-2[1]
体外研究
(In Vitro)

Dencichin (β-ODAP, 10 μM, 50 μM, 100 μM and 200 μM) increases HRE expression by 1.3±0.09, 2.5±0.07, 4.2±0.15 and 1.3±0.07 fold respectively compared to control. Dencichin has intermolecular interactions with PHD-2[1]. Dencichin (10 μM, 100 μM, 1 mM) significantly inhibits cell proliferation and extracellular matrix (ECM) proteins accumulation of HBZY-1 cells, and reduces the secretion of collagen I (Col I), collagen IV (Col IV), and fibronectin (FN)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Dencichine 相关抗体:
体内研究
(In Vivo)

Dencichin improves metabolism disorder in diabetic nephropathy (DN) secondary to type II diabetes mellitus (DM) model. Dencichin (80, 160 mg/kg/day, p.o.) significantly prevents the up-regulation of TCH, TG, LDL, and HbAlc and the down-regulation of HDL in DN rats induced by STZ injection. Dencichin also attenuates renal injury induced in the DN secondary to type II DM model. Dencichin alleviates pancreas damage in the STZ-induced DN model. Dencichin regulates protein expression in the TGF-β/Smad signalling pathway in STZ-induced DN models[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

176.13

Formula

C5H8N2O5
CAS 号
性状

固体

颜色

White to off-white

中文名称

三七素
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

H2O 中的溶解度 : 5 mg/mL (28.39 mM; 超声助溶)

DMSO 中的溶解度 : 5 mg/mL (28.39 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

0.1 M HCL 中的溶解度 : 3.33 mg/mL (18.91 mM; 超声助溶 (<60°C))

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 5.6776 mL 28.3881 mL 56.7762 mL
5 mM 1.1355 mL 5.6776 mL 11.3552 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 98.93%

COA (190 KB) HNMR (252 KB) LCMS (87 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[2]

Cells are cultured in 6-well plates with glucose (LG) or high glucose control (HG) and then treated with valsartan (1.0 × 10-3 M) or Dencichin (1.0 × 10-5 M, 1.0 × 10-4 M and 1.0 × 10-3 M) for 24 h before analysing the activation of FN, Col I, and Col IV expression by ELISA and analysing MMP-9 and TIMP-1 expression by immunofluorescence. Each experiment is repeated at least three times throughout the study[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Male SD rats weighted approximately 200 ± 20 g (eight weeks old) are kept at 23 ± 2°C. The rats are randomLy allotted into two groups: normal group (n = 16) and diabetic nephropathy (DN) group (n = 60). The normal rats fed with normal diet and vehicle fall into the normal control group (NC, n = 8) and the normal rats fed with normal diet and Dencichin fall into the Dencichin control group (De C, n = 8). The rats in the diabetic group are fed the high-sugar-high-fat diet (composition: Common breeding material 54.6%, lard oil 16.9%, sucrose 14%, casein 10.2%, gunk 2.1%, maltodextrin 2.2%). Five weeks later, DN is induced by administering of 40 mg/kg STZ though intraperitoneal injection, and the normal rats are treated with vehiclecitrate buffer (0.1 M, pH 4.2). Next, the DN rats (n = 32) are assigned into four groups and oral administered with metformin hydrochloride (a positive control) at 80 mg/kg/day, (DN+Met, n = 8), which is dissolve into distilled water to make a 2 mg/mL solution before use; vehicle control for the DN control group (DN, n = 8), or Dencichin. The Dencichin group is divided into a high Dencichin group (160 mg/kg/day; DN+De H, n = 8) and a low Dencichin group (80 mg/kg/day; DN+De L, n = 8), which are dissolved into distilled water to make a 5 mg/mL and 2.5 mg/mL solution before use and oral administered at the dosage once per day. Blood glucose is measured each month. 8 w after administering with Dencichin, the rats are killed[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Dencichine 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
0.1 M HCL / H2O / DMSO 1 mM 5.6776 mL 28.3881 mL 56.7762 mL 141.9406 mL
5 mM 1.1355 mL 5.6776 mL 11.3552 mL 28.3881 mL
10 mM 0.5678 mL 2.8388 mL 5.6776 mL 14.1941 mL
15 mM 0.3785 mL 1.8925 mL 3.7851 mL 9.4627 mL
H2O / DMSO 20 mM 0.2839 mL 1.4194 mL 2.8388 mL 7.0970 mL
25 mM 0.2271 mL 1.1355 mL 2.2710 mL 5.6776 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dencichine5302-45-4Dencichin ODAP三七素HIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHInhibitorinhibitorinhibit

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