1. 多肽产品
  2. 多肽及其衍生物
  3. 肽表位
  4. OVA Peptide(257-264)

OVA Peptide(257-264) 是 OVA 的 I 类 (Kb)-限制性肽表位,它是一种八聚体肽,可以来自 I 类 MHC 分子 H-2Kb 所呈现的卵清蛋白。

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Custom Peptide Synthesis

OVA Peptide(257-264) Chemical Structure

OVA Peptide(257-264) Chemical Structure

CAS No. : 138831-86-4

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
1 mg ¥720
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5 mg ¥1360
In-stock
10 mg ¥2160
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100 mg   询价  

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Customer Review

Other Forms of OVA Peptide(257-264):

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

OVA Peptide(257-264) is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb.

体外研究
(In Vitro)

TAP1-I- and C57BL/6 macrophages may process Crl-OVA and full-length OVA in different cellular compartments and that the protein context of the OVA Peptide(257-264) epitope influences the extent of TAP-independent processing for MHC class I presentation. OVA Peptide(257-264) epitope is presented with a differential dependence on the TAP transporter depending on the protein context of the OVA epitope: OVA Peptide(257-264) contained within the MBPCrl-OVA or Crl-OVA bacterial fusion proteins is presented with little dependence on the TAP transporter, while OVA Peptide(257-264) contained within full-length ovalbumin is largely dependent on the TAP transporter, regardless of whether recombinant OVA is expressed in bacteria or the native protein is coupled to polystyrene beads[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

963.13

Formula

C45H74N10O13

CAS 号
性状

固体

颜色

White to off-white

Sequence

Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu

Sequence Shortening

SIINFEKL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : 40 mg/mL (41.53 mM; ultrasonic and adjust pH to 3 with 1 M HCL; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

0.1% TFA in Water 中的溶解度 : 1.83 mg/mL (1.90 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0383 mL 5.1914 mL 10.3828 mL
5 mM 0.2077 mL 1.0383 mL 2.0766 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.68%

参考文献
Cell Assay

TAPl-/- or C57BL/6 macrophages are co-incubated with either bacteria or polystyrene beads containing the 257-264 epitope from ovalbumin [OVA Peptide(257-264)], which binds the mouse class I molecule Kb. The source of the OVA(257-264) epitope is either the Crl-OVA(257-264) (Crl-OVA) fusion protein, the maltose binding protein (MBP)-Crl-OVA fusion protein, native OVA or bacterial recombinant OVA (rOVA); Crl-OVA, MBP-Crl-OVA and rOVA are each expressed in bacteria, and Crl-OVA and MBP-Crl-OVA purified from bacterial lysates and native egg OVA are coated onto polystyrene beads[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
0.1% TFA in Water / DMSO 1 mM 1.0383 mL 5.1914 mL 10.3828 mL 25.9570 mL
DMSO 5 mM 0.2077 mL 1.0383 mL 2.0766 mL 5.1914 mL
10 mM 0.1038 mL 0.5191 mL 1.0383 mL 2.5957 mL
15 mM 0.0692 mL 0.3461 mL 0.6922 mL 1.7305 mL
20 mM 0.0519 mL 0.2596 mL 0.5191 mL 1.2979 mL
25 mM 0.0415 mL 0.2077 mL 0.4153 mL 1.0383 mL
30 mM 0.0346 mL 0.1730 mL 0.3461 mL 0.8652 mL
40 mM 0.0260 mL 0.1298 mL 0.2596 mL 0.6489 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
OVA Peptide(257-264)
目录号:
HY-P1489
需求量: