2. 诱导疾病模型产品 Stem Cell/Wnt GPCR/G Protein Metabolic Enzyme/Protease
  3. 神经系统疾病模型 Organoid Prostaglandin Receptor Endogenous Metabolite
  4. 神经性疼痛模型
  5. Prostaglandin E2

Prostaglandin E2  (Synonyms: 前列腺素 E2; PGE2; Dinoprostone)

目录号: HY-101952 纯度: 99.74%
COA 产品使用指南

摩尔计算器

Prostaglandin E2 (PGE2) 是一种激素样物质，参与广泛的身体功能，如平滑肌的收缩和松弛，血管的扩张和收缩，血压的控制和炎症的调节。

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Prostaglandin E2 Chemical Structure

Prostaglandin E2 Chemical Structure

CAS No. : 363-24-6

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥770
In-stock
5 mg ￥700
In-stock
10 mg ￥1100
In-stock
25 mg ￥2200
In-stock
50 mg ￥3100
In-stock
100 mg ￥3900
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Prostaglandin E2:

Prostaglandin E2 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 31 篇科研文献

查看 Prostaglandin Receptor 亚型特异性产品:

查看所有亚型
DP EP FP IP TXA2/TP TXB2

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Prostaglandin E2 (PGE2) is a hormone-like substance that participate in a wide range of body functions such as the contraction and relaxation of smooth muscle, the dilation and constriction of blood vessels, control of blood pressure, and modulation of inflammation.

IC50 & Target[1]

EP

 

Human Endogenous Metabolite

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
CHO EC50
1.9 nM
Compound: Prostaglandin E2
Agonist activity at human EP2 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method
Agonist activity at human EP2 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method
[PMID: 26985320]
CHO EC50
2.5 nM
Compound: Prostaglandin E2
Agonist activity at human EP3 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis
Agonist activity at human EP3 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis
[PMID: 26985320]
CHO EC50
3.7 nM
Compound: Prostaglandin E2
Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis
Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis
[PMID: 26985320]
CHO EC50
347 nM
Compound: Prostaglandin E2
Agonist activity at human IP receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method
Agonist activity at human IP receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method
[PMID: 26985320]
CHO EC50
7.5 nM
Compound: Prostaglandin E2
Agonist activity at human EP4 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method
Agonist activity at human EP4 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method
[PMID: 26985320]
HEK293 IC50
2.6 nM
Compound: PGE2
Displacement of [3H]PGE2 from human recombinant EP2 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method
Displacement of [3H]PGE2 from human recombinant EP2 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method
[PMID: 27876250]
HEK293 EC50
346 nM
Compound: Prostaglandin E2
Agonist activity at PK2-tagged human EP2 receptor expressed in HEK293 cells assessed as induction of EA-tagged beta-arrestin recruitment incubated for 90 mins by beta-galactosidase reporter gene assay
Agonist activity at PK2-tagged human EP2 receptor expressed in HEK293 cells assessed as induction of EA-tagged beta-arrestin recruitment incubated for 90 mins by beta-galactosidase reporter gene assay
[PMID: 26985320]
HEK293 IC50
4 nM
Compound: PGE2
Displacement of [3H]prostaglandin E2 from human EP2 receptor expressed in HEK293 cell membranes after 60 mins by liquid scintillation counting
Displacement of [3H]prostaglandin E2 from human EP2 receptor expressed in HEK293 cell membranes after 60 mins by liquid scintillation counting
[PMID: 29995405]
HEK293 IC50
5.5 x 10-10 M
Compound: PGE2
Displacement of [3H]PGE2 from human recombinant EP4 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method
Displacement of [3H]PGE2 from human recombinant EP4 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method
[PMID: 27876250]
体外研究
(In Vitro)

PGE2 显示在受照射和未受照射的 T 淋巴细胞的混合物中抑制 IL 2 的产生。PGE2 (0.1-10 μM) 剂量依赖性地抑制 IL 2 的产生。PGE2 在抑制细胞激活的诱导期起作用。将 T 淋巴细胞与 PGE2 预孵育可诱导抑制 IL 2 生成和 PHA 增殖的细胞[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Prostaglandin E2 相关抗体:
体内研究
(In Vivo)

Prostaglandin E2 可用于动物建模，构建大鼠疼痛模型。

PGE2 (0.3 μg/k，ip) 显著减少大鼠体内正在吞噬甲基丙烯酸酯微珠的腹膜巨噬细胞数量[2]。PGE2 (0.1 mg/min，ia) 增加肾血流量。PGE2 在肾血管阻力中产生双相变化，血管舒张从 0.01 mg/min 开始，在约 3 mg/min 时达到最大，而在使用的最高剂量 (20 mg/min) 时，PGE2 诱导肾血管收缩[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

352.47

Formula

C20H32O5
CAS 号
性状

固体

颜色

White to off-white

中文名称

前列腺素 E2；地诺前列酮；前列腺素E2
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (283.71 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H2O 中的溶解度 : 1 mg/mL (2.84 mM; 超声助溶 (<60°C))

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.8371 mL 14.1856 mL 28.3712 mL
5 mM 0.5674 mL 2.8371 mL 5.6742 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.09 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.09 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.76%

COA (198 KB) HNMR (284 KB) RP-HPLC (242 KB) MS (69 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[1]

Lymphocytes in CM (1×106 cells/mL) are ditributed in microculture plates (100 μL) in triplicate in the presence of PGE-treated T cells or medium-treated T cells and stimulated with PHA-P at various mitogenic doses. After 72 hr, cultures are pulsed with 1 μCi [3H]thymidine per well (specific activity 5 Ci/mM) for 16 to 18 hr, collected with amicroprecipltator, dried, and counted in a liquid scintillation counter.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Male Sprague Dawley rats (200-250 g) are used throughout the study. For 3 consecutive days rats in the experimental groups receive a daily intraperitoneal injection of either PGE2 (0.3 μg/kg body weight (BW)), the prostaglandin inhibitor mecbofenamate (10 mg/kg BW) or the prostaglandin precursor arachidonic acid (0.3 μg/ kg BW). To determine whether or not 0.3 μg/kg BW of a fatty acid produces nonspecific effects, the biologically inactive fatty acid 11, 14, 17-eicosatrienoic acid is also administered to a group of rats. Rats in the control group receive an equivalent volume (2.0 mL/kg BW) of the vehicle. On the third day, 3 mL of a suspension containing 1.2×106 fluorescent methacrylate microbeads/mL of PBS are injected intraperitoneally (ip) into each rat. Six hours later all animals are given ip a regular dose of their respective treatment. Peritoneal exudate cells are harvested 19-22 hr later.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Prostaglandin E2 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.8371 mL 14.1856 mL 28.3712 mL 70.9280 mL
DMSO 5 mM 0.5674 mL 2.8371 mL 5.6742 mL 14.1856 mL
10 mM 0.2837 mL 1.4186 mL 2.8371 mL 7.0928 mL
15 mM 0.1891 mL 0.9457 mL 1.8914 mL 4.7285 mL
20 mM 0.1419 mL 0.7093 mL 1.4186 mL 3.5464 mL
25 mM 0.1135 mL 0.5674 mL 1.1348 mL 2.8371 mL
30 mM 0.0946 mL 0.4729 mL 0.9457 mL 2.3643 mL
40 mM 0.0709 mL 0.3546 mL 0.7093 mL 1.7732 mL
50 mM 0.0567 mL 0.2837 mL 0.5674 mL 1.4186 mL
60 mM 0.0473 mL 0.2364 mL 0.4729 mL 1.1821 mL
80 mM 0.0355 mL 0.1773 mL 0.3546 mL 0.8866 mL
100 mM 0.0284 mL 0.1419 mL 0.2837 mL 0.7093 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Prostaglandin E2363-24-6PGE2 Dinoprostone前列腺素 E2地诺前列酮前列腺素E2Prostaglandin E 2Prostaglandin E-2PGE 2PGE-2OrganoidProstaglandin ReceptorEndogenous MetaboliteInhibitorinhibitorinhibit

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