2. Cell Cycle/DNA Damage Anti-infection Autophagy Apoptosis
  3. Topoisomerase Bacterial Autophagy Apoptosis
  4. Etoposide phosphate

Etoposide phosphate  (Synonyms: 磷酸依托泊苷; BMY-40481)

目录号: HY-13630 纯度: 96.16%
COA 产品使用指南

摩尔计算器

Etoposide phosphate (BMY-40481) 是一种有效的抗癌 (anti-cancer) 化疗试剂和一种选择性拓扑异构酶 II (topoisomerase II) 抑制剂，可以防止 DNA 链的重新连接。Etoposide phosphate 是依托泊苷的磷酸酯前体，被认为与 Etoposide 活性相当。Etoposide phosphate 诱导细胞周期阻滞、凋亡 (apoptosis) 和自噬 (autophagy)。

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Etoposide phosphate Chemical Structure

Etoposide phosphate Chemical Structure

CAS No. : 117091-64-2

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥1177
In-stock
5 mg ￥800
In-stock
10 mg ￥1300
In-stock
25 mg ￥2600
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50 mg ￥4100
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Customer Review

Other Forms of Etoposide phosphate:

Etoposide phosphate 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 97 篇科研文献

WB
RT-PCR
IF
Proliferation Assay
Cell Viability Assay

    Etoposide phosphate purchased from MCE. Usage Cited in: Oncogene. 2023 Feb 2.  [Abstract]

    Etoposide (VP16; 5 mg/kg; s.c.; every two days for 8 days) strongly suppresses tumor growth in mice.

    Etoposide phosphate purchased from MCE. Usage Cited in: J Pharm Pharmacol. 2023 Mar 1;rgad007.  [Abstract]

    Irinotecan (CPT11; 20 μM; 48 h) or Etoposide (VP16; 20 μM; 48 h) induces MDA-MB-231 cells cycle arrest significantly.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    Etoposide induced BCL6 protein expression in a time-dependent manner. ETO-resistant or -sensitive cells are treated with etoposide at their respective 1/4 IC50s for 2, 4, or 6 days.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    STAT1 protein and its phosphorylation levels by immunoblotting analysis. Etoposide (ETO)-resistant and -sensitive cells are treated with Etoposide at their respective 1/2 IC50s for indicated time points.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    Etoposide activated mTOR signaling components in Etoposide-resistant Capan-2 and PANC28 cells. Cells were treated with etoposide at their respective 1/2 IC50s for 6 or 12 hr. A long-term treatment with Etoposide activated mTOR signaling components in ETO-resistant cells. Capan-2 and PANC28 cells are treated with 10 μM Etoposide for 2 or 4 days.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    BCL6 mRNA expression in Etoposide (ETO)-resistant and -sensitive cells. Cells are treated with Etoposide at their respective 1/2 IC50s for 24 hr.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    Cell sensitivity to Etoposide (ETO). Cancer cells are treated with etoposide at gradient concentrations for 48 hr.

    Etoposide phosphate purchased from MCE. Usage Cited in: Protein Cell. 2022 Jan;13(1):47-64.  [Abstract]

    FISH of telomeres on metaphase spreads to detect chromosome end-to-end fusions in 6 kb-HeLa treated with VP-16. Control (Scr) or cGAS-depleted cells (gcGAS-1, gcGAS-2) are either harvested immediately following treatment (VP-16, 2 μM, 1 h) or released for 4 h (Release 4 h) during mitosis.

    Etoposide phosphate purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Mar 12;12(3):265.  [Abstract]

    Inhibition of PRDX1 increased the sensitivity of HCT116 cells to Etoposide-induced apoptosis: HCT116 cells infected with lentivirus expressing the indicated shRNAs were treated with 50 μM Etoposide for 0-6 h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Sep 28;7(21):2001364.  [Abstract]

    Lnc‐Ip53 attenuated the DNA damage- and oxidation stress-induced p53 acetylation at K382. Cells are incubated with 50 µM Etoposide (Eto) for 5 h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Sep 28;7(21):2001364.  [Abstract]

    Lnc‐Ip53 attenuated the DNA damage‐ and oxidation stress‐triggered increase of CDKN1A and PUMA. Cells are incubated with 50 µM Etoposide (Eto) for 6 h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Hepatology. 2020 May;71(5):1660-1677.  [Abstract]

    Exposure to Etoposide (Eto), another DNA-damaging agent, induces PDIA3P1 expression, suggesting that PDIA3P1 may be up-regulated by DNA-damaging agents.

    Etoposide phosphate purchased from MCE. Usage Cited in: Theranostics. 2020 Jul 25;10(21):9477-9494.  [Abstract]

    When cells are treated with 20 µM Etoposide, an obvious increased green focus of RAD51 is observed in the treated fibroblasts using immunofluorescence (IF) staining.

    Etoposide phosphate purchased from MCE. Usage Cited in: Theranostics. 2020 Jul 25;10(21):9477-9494.  [Abstract]

    Fibroblasts and HT-1080 cells are treated with 20 µM Etoposide or DMSO (vehicle group) for 2, 4, 8, 16, 24 and 36 h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Theranostics. 2020 Jul 25;10(21):9477-9494.  [Abstract]

    When cells are treated with 20 µM Etoposide or DMSO (vehicle group) for 4 h, western blots (WB) shows elevated expression of γH2AX, a marker of double-strand breaks (DSBs), in the Etoposide group.

    Etoposide phosphate purchased from MCE. Usage Cited in: EMBO Mol Med. 2020 Nov 6;12(11):e12525.  [Abstract]

    Immunoblot of PARP1 cleavage of PeTa cells after 6 days of indicated treatment. Etoposide (10 μM; for 24 h) serves as positive control for apoptosis, and H3 serves as loading control.

    Etoposide phosphate purchased from MCE. Usage Cited in: Aging Cell. 2020 Jul;19(7):e13171.  [Abstract]

    RPE p16‐mCherry cells were treated with DMSO, Etoposide (20 μM), H2O2 (200 μM), or serum starved for 24 hr.

    Etoposide phosphate purchased from MCE. Usage Cited in: Leuk Lymphoma. 2018 Jan;59(1):162-170.  [Abstract]

    WT1 and caspase-3 protein levels are detected in two primary AML blasts treated with 100 μM Eto for 24 h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Cancer Lett. 2017 Nov 1;408:43-54.  [Abstract]

    Western blot analysis of p-p70S6k, p70S6k, p-AKT and AKT after 6 h of treatment with 20 μM RAD001. Levels of p-p70S6k and p-AKT are quantified by densitometric analysis and a corresponding histogram is constructed as relative to p70S6k or AKT and α-tubulin. The lower panel shows a representative Western blot.

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    Topoisomerase Topo I Topo II

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Etoposide phosphate (BMY-40481) is a potent anti-cancer chemotherapy agent and a selective topoisomerase II inhibitor to prevent re-ligation of DNA strands. Etoposide phosphate is the phosphate ester proagent of etoposide and is considered as active equivalent to Etoposide. Etoposide phosphate induces cell cycle arrest, apoptosis, and autophagy.

    IC50 & Target[1]

    Topoisomerase II

     

    体外研究
    (In Vitro)

    Etoposide phosphate 是依托泊苷的水溶性衍生物，可能是依托泊苷的前体药物，其特征在于在依托泊苷分子 E 环的 4' 位存在磷酸基团[1]。
    Etoposide phosphate (0-1 μM；72 小时) 抑制 HCT116 FBXW+/+、FBXW-/- 和 p53-/- 作为剂量-依赖方式，表现出 IC50 值为 0.945 μM；0.375 微米；和 1.437 μM[2]。
    Etoposide phosphate (25 μM；6 小时) 延迟 FBXW7 缺陷细胞中的 p53 恢复。此外，FBXW7-/- 细胞中 FBXW7 表达消失[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Etoposide phosphate 相关抗体:

    Cell Viability Assay[2]

    Cell Line: FBXW+/+, FBXW-/- and p53-/- cell
    Concentration: 0.025 μM, 0.05 μM, 0.075 μM, 0.1 μM, 0.2 μM, 0.4 μM, 0.6 μM, 0.8 μM, 1 μM
    Incubation Time: 72 hours
    Result: Inhibited HCT116 FBXW+/+, FBXW-/- and p53-/- cell growth as a concentration manner.

    Western Blot Analysis[2]

    Cell Line: HCT116 FBXW7+/+ or FBXW7-/- cells
    Concentration: 25 μM
    Incubation Time: 6 hours
    Result: Exhibited that the recovery of p53 levels after DNA damage is mediated by FBXW7.
    体内研究
    (In Vivo)

    磷酸依托泊苷 (静脉注射；50、100 或 150 mg/kg；单剂量) 在第 8 天雌性 CD-1 小鼠中出现进行性共济失调、翻正反射受损、前后肢张开和麻痹的临床症状[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female CD-1 mice[3]
    Dosage: 50, 100, or 150 mg/kg
    Administration: Intravenous injection; single dose
    Result: Observed degeneration of dorsal root ganglion cells and axonal degeneration of their distal and proximal processes in peripheral nerves, dorsal spinal roots, and dorsal funiculi of the spinal cord at all doses under light microscopy (LM).
    Clinical Trial
    分子量

    668.54

    Formula

    C29H33O16P
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    磷酸依托泊苷
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 100 mg/mL (149.58 mM; 超声助溶 (<80°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : 100 mg/mL (149.58 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 1.4958 mL 7.4790 mL 14.9580 mL
    5 mM 0.2992 mL 1.4958 mL 2.9916 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 100 mg/mL (149.58 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料
    参考文献

    Etoposide phosphate 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.4958 mL 7.4790 mL 14.9580 mL 37.3949 mL
    5 mM 0.2992 mL 1.4958 mL 2.9916 mL 7.4790 mL
    10 mM 0.1496 mL 0.7479 mL 1.4958 mL 3.7395 mL
    15 mM 0.0997 mL 0.4986 mL 0.9972 mL 2.4930 mL
    20 mM 0.0748 mL 0.3739 mL 0.7479 mL 1.8697 mL
    25 mM 0.0598 mL 0.2992 mL 0.5983 mL 1.4958 mL
    30 mM 0.0499 mL 0.2493 mL 0.4986 mL 1.2465 mL
    40 mM 0.0374 mL 0.1870 mL 0.3739 mL 0.9349 mL
    50 mM 0.0299 mL 0.1496 mL 0.2992 mL 0.7479 mL
    60 mM 0.0249 mL 0.1246 mL 0.2493 mL 0.6232 mL
    80 mM 0.0187 mL 0.0935 mL 0.1870 mL 0.4674 mL
    100 mM 0.0150 mL 0.0748 mL 0.1496 mL 0.3739 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Etoposide117091-64-2BMY-40481磷酸依托泊苷BMY40481BMY 40481TopoisomeraseBacterialAutophagyApoptosisHCT116prodrugFBXWp53anti-cancerchemotherapyapoptosisP388leukemiaperipheralneuropathyInhibitorinhibitorinhibit

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