PFI-1

目录号: HY-16586 纯度: 99.87%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

PFI-1 是一种有效的 BET 抑制剂，能够有效抑制 BRD4，IC₅₀ 值为 0.22 μM。

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PFI-1 Chemical Structure

CAS No. : 1403764-72-6

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥484	In-stock
1 mg	￥176	In-stock
5 mg	￥440	In-stock
10 mg	￥800	In-stock
50 mg	￥2200	In-stock
100 mg	￥4000	In-stock
200 mg		询价
500 mg		询价

or 大包装询价

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Customer Review

MCE 顾客使用本产品发表的 2 篇科研文献

PFI-1 相关产品

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•同靶点产品:

•同靶点蛋白产品:

生物活性
纯度 & 产品资料
参考文献

生物活性

PFI-1 is a selective BET (bromodomain-containing protein) inhibitor for BRD4 with IC₅₀ of 0.22 μM in a cell-free assay.

IC₅₀ & Target

IC50: 0.22 μM (BRD4)

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
786-0	GI₅₀	12 μM Compound: 7; PFI-1	Growth inhibition of human 786-0 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human 786-0 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
A498	GI₅₀	1.8 μM Compound: 7; PFI-1	Growth inhibition of human A498 cells after 48 hrs by SRB assay Growth inhibition of human A498 cells after 48 hrs by SRB assay	[PMID: 29776834]
A549	GI₅₀	19.5 μM Compound: 7; PFI-1	Growth inhibition of human A549/ATCC cells after 48 hrs by sulforhodamine B assay Growth inhibition of human A549/ATCC cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
ACHN	GI₅₀	7.8 μM Compound: 7; PFI-1	Growth inhibition of human ACHN cells after 48 hrs by sulforhodamine B assay Growth inhibition of human ACHN cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
BT-549	GI₅₀	14.8 μM Compound: 7; PFI-1	Growth inhibition of human BT549 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human BT549 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
CAKI-1	GI₅₀	3.6 μM Compound: 7; PFI-1	Growth inhibition of human CAKI-1 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human CAKI-1 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
CCRF-CEM	GI₅₀	7.8 μM Compound: 7; PFI-1	Growth inhibition of human CCRF-CEM cells after 48 hrs by sulforhodamine B assay Growth inhibition of human CCRF-CEM cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
COLO 205	GI₅₀	3 μM Compound: 7; PFI-1	Growth inhibition of human COLO205 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human COLO205 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
DU-145	GI₅₀	28.8 μM Compound: 7; PFI-1	Growth inhibition of human DU145 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human DU145 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HCC 2998	GI₅₀	9.1 μM Compound: 7; PFI-1	Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HCC 2998	GI₅₀	93.3 μM Compound: 7; PFI-1	Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HCT-116	GI₅₀	4.5 μM Compound: 7; PFI-1	Growth inhibition of human HCT116 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HCT116 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HCT-15	GI₅₀	3.2 μM Compound: 7; PFI-1	Growth inhibition of human HCT15 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HCT15 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HL-60	IC₅₀	2.19 μM Compound: 1; PFI-1	Cytotoxicity against human HL60 cells assessed as decrease in cell viability after 24 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 27266999]
HL-60	IC₅₀	3.99 μM Compound: PFI-1	Antiproliferative activity against human HL60 cells incubated for 72 hrs by CCK8 assay Antiproliferative activity against human HL60 cells incubated for 72 hrs by CCK8 assay	[PMID: 32883643]
HL-60(TB)	GI₅₀	3.4 μM Compound: 7; PFI-1	Growth inhibition of human HL-60(TB) cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HL-60(TB) cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HOP-62	GI₅₀	2.5 μM Compound: 7; PFI-1	Growth inhibition of human HOP62 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HOP62 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HOP-92	GI₅₀	1.2 μM Compound: 7; PFI-1	Growth inhibition of human HOP92 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HOP92 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
Hs-578T	GI₅₀	1.9 μM Compound: 7; PFI-1	Growth inhibition of human Hs 578T cells after 48 hrs by sulforhodamine B assay Growth inhibition of human Hs 578T cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HT-29	IC₅₀	14.72 μM Compound: PFI-1	Antiproliferative activity against human HT29 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human HT29 cells incubated for 72 hrs by MTT assay	[PMID: 32883643]
HT-29	GI₅₀	3 μM Compound: 7; PFI-1	Growth inhibition of human HT-29 cells after 48 hrs by SRB assay Growth inhibition of human HT-29 cells after 48 hrs by SRB assay	[PMID: 29776834]
IGROV-1	GI₅₀	6.3 μM Compound: 7; PFI-1	Growth inhibition of human IGROV1 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human IGROV1 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
K562	GI₅₀	4.6 μM Compound: 7; PFI-1	Growth inhibition of human K562 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human K562 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
KM12	GI₅₀	10.7 μM Compound: 7; PFI-1	Growth inhibition of human KM12 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human KM12 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
LOX IMVI	GI₅₀	3.5 μM Compound: 7; PFI-1	Growth inhibition of human LOXIMVI cells after 48 hrs by sulforhodamine B assay Growth inhibition of human LOXIMVI cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
M14	GI₅₀	2.9 μM Compound: 7; PFI-1	Growth inhibition of human M14 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human M14 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MCF7	GI₅₀	1.5 μM Compound: 7; PFI-1	Growth inhibition of human MCF7 cells after 48 hrs by SRB assay Growth inhibition of human MCF7 cells after 48 hrs by SRB assay	[PMID: 29776834]
MDA-MB-231	GI₅₀	3.5 μM Compound: 7; PFI-1	Growth inhibition of human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MDA-MB-435	GI₅₀	2.6 μM Compound: 7; PFI-1	Growth inhibition of human MDA-MB-435 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human MDA-MB-435 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MDA-MB-468	GI₅₀	1.5 μM Compound: 7; PFI-1	Growth inhibition of human MDA-MB-468 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human MDA-MB-468 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MOLT-4	GI₅₀	10.5 μM Compound: 7; PFI-1	Growth inhibition of human MOLT4 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human MOLT4 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MV4-11	IC₅₀	7.75 μM Compound: 1; PFI-1	Cytotoxicity against human MV4-11 cells assessed as decrease in cell viability after 24 hrs by MTT assay Cytotoxicity against human MV4-11 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 27266999]
NCI/ADR-RES	GI₅₀	17.4 μM Compound: 7; PFI-1	Growth inhibition of human NCI-ADR-RES cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-ADR-RES cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H226	GI₅₀	14.1 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H226 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H226 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H23	GI₅₀	8.5 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H23 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H23 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H322M	GI₅₀	6 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H322M cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H322M cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H460	GI₅₀	9.3 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H460 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H460 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H522	GI₅₀	8.7 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H522 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H522 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
OVCAR-3	GI₅₀	4 μM Compound: 7; PFI-1	Growth inhibition of human OVCAR3 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR3 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
OVCAR-4	GI₅₀	6.3 μM Compound: 7; PFI-1	Growth inhibition of human OVCAR4 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR4 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
OVCAR-5	GI₅₀	10.5 μM Compound: 7; PFI-1	Growth inhibition of human OVCAR5 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR5 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
OVCAR-8	GI₅₀	9.1 μM Compound: 7; PFI-1	Growth inhibition of human OVCAR8 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR8 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
PBMC	EC₅₀	1.89 μM Compound: 17, PFI-1, PF-6405761, 4E96	Inhibition of LPS-induced IL6 production in human PBMC by ELISA Inhibition of LPS-induced IL6 production in human PBMC by ELISA	[PMID: 23095041]
PC-3	GI₅₀	28.2 μM Compound: 7; PFI-1	Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
RPMI-8226	GI₅₀	2.2 μM Compound: 7; PFI-1	Growth inhibition of human RPMI8226 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human RPMI8226 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
RXF 393	GI₅₀	7.9 μM Compound: 7; PFI-1	Growth inhibition of human RXF393 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human RXF393 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SF-268	GI₅₀	28.8 μM Compound: 7; PFI-1	Growth inhibition of human SF268 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SF268 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SF-295	GI₅₀	13.5 μM Compound: 7; PFI-1	Growth inhibition of human SF295 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SF295 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SF-539	GI₅₀	4.5 μM Compound: 7; PFI-1	Growth inhibition of human SF539 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SF539 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SK-MEL-2	GI₅₀	29.5 μM Compound: 7; PFI-1	Growth inhibition of human SK-MEL-2 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SK-MEL-2 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SK-MEL-28	GI₅₀	6.2 μM Compound: 7; PFI-1	Growth inhibition of human SK-MEL-28 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SK-MEL-28 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SK-MEL-5	GI₅₀	6.3 μM Compound: 7; PFI-1	Growth inhibition of human SK-MEL-5 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SK-MEL-5 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SK-OV-3	GI₅₀	4.9 μM Compound: 7; PFI-1	Growth inhibition of human SKOV3 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SKOV3 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SN12C	GI₅₀	6.8 μM Compound: 7; PFI-1	Growth inhibition of human SN12C cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SN12C cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SNB-19	GI₅₀	4.8 μM Compound: 7; PFI-1	Growth inhibition of human SNB19 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SNB19 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SNB-75	GI₅₀	0.4 μM Compound: 7; PFI-1	Growth inhibition of human SNB75 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SNB75 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SR	GI₅₀	3.7 μM Compound: 7; PFI-1	Growth inhibition of human SR cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SR cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SW-620	GI₅₀	12 μM Compound: 7; PFI-1	Growth inhibition of human SW620 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SW620 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
T47D	GI₅₀	3.7 μM Compound: 7; PFI-1	Growth inhibition of human T47D cells after 48 hrs by sulforhodamine B assay Growth inhibition of human T47D cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
THP-1	IC₅₀	0.43 μM Compound: 1; PFI-1	Antiinflammatory activity against human THP-1 cells assessed as inhibition of LPS-induced IL-6 production incubated for 18 hrs by ELISA Antiinflammatory activity against human THP-1 cells assessed as inhibition of LPS-induced IL-6 production incubated for 18 hrs by ELISA	[PMID: 35318165]
TK-10	GI₅₀	1.6 μM Compound: 7; PFI-1	Growth inhibition of human TK10 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human TK10 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
U-251	GI₅₀	15.1 μM Compound: 7; PFI-1	Growth inhibition of human U251 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human U251 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
U-937	IC₅₀	> 20 μM Compound: 1; PFI-1	Cytotoxicity against human U937 cells assessed as decrease in cell viability after 24 hrs by MTT assay Cytotoxicity against human U937 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 27266999]
UACC-257	GI₅₀	3.6 μM Compound: 7; PFI-1	Growth inhibition of human UACC257 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human UACC257 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
UACC-62	GI₅₀	0.7 μM Compound: 7; PFI-1	Growth inhibition of human UACC62 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human UACC62 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
UO-31	GI₅₀	1.1 μM Compound: 7; PFI-1	Growth inhibition of human UO31 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human UO31 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
WI-38	IC₅₀	19.8 μM Compound: PFI-1	Antiproliferative activity against human WI-38 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human WI-38 cells incubated for 72 hrs by MTT assay	[PMID: 32883643]

体外研究
(In Vitro)

PFI-1对白血病细胞系具有抗增殖作用，并能有效抑制其克隆生长。敏感细胞系暴露于PFI-1后会导致G1期细胞周期停滞、MYC表达下调以及诱导细胞凋亡，并促使原发性白血病母细胞分化。暴露于PFI-1的细胞显示出Aurora B激酶显著下调，从而减弱了Aurora底物H3S10的磷酸化，这为特异性抑制这一公认的肿瘤学靶点提供了另一种策略^[1]。^[2] PFI-1与环腺苷酸反应结合蛋白结合，解离常数K_d为49 μM。PFI-1抑制LPS刺激的人血液单核细胞产生IL-6的EC₅₀值为1.89 μM^[2]。^[2] PFI-1在T4302 CD133⁺细胞中引起剂量依赖性的细胞存活率下降^[3]。^[2] PFI-1能够抑制三种神经内分泌肿瘤（NET）细胞系的增殖，包括源自胰腺NET的Bon-1细胞系以及源自肺部NET的H727和H720细胞系^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

在大鼠中给予 PFI-1 (1 mg/kg，iv) 导致分布容积为 1 L/kg，血浆清除率为 18 mL/min/kg，半衰期为 1 小时。大鼠口服 PFI-1 (2 mg/kg) 导致口服生物利用度低至 32%。在小鼠中给予 PFI-1 (2 mg/kg，sc) 导致 Cmax 为 58 ng/mL，Tmax 为 1 小时，半衰期约为 2 小时^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

347.39

Formula

C₁₆H₁₇N₃O₄S

CAS 号

1403764-72-6

性状

固体

颜色

Light yellow to yellow

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 33.33 mg/mL (95.94 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8786 mL	14.3930 mL	28.7861 mL
5 mM	0.5757 mL	2.8786 mL	5.7572 mL
10 mM	0.2879 mL	1.4393 mL	2.8786 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.20 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.20 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.87%

选择批次：

Data Sheet (651 KB) SDS (393 KB)

COA (288 KB) LCMS (93 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Picaud S, et al. PFI-1, a Highly Selective Protein Interaction Inhibitor, Targeting BET Bromodomains. Cancer Res. 2013 May 21. [Epub ahead of print] [Content Brief]

[2]. Fish PV, et al. Identification of a chemical probe for bromo and extra C-terminal bromodomain inhibition through optimization of a fragment-derived hit. J Med Chem. 2012 Nov 26;55(22):9831-7. [Content Brief]

[3]. Cheng Z, et al. Inhibition of BET bromodomain targets genetically diverse glioblastoma. Clin Cancer Res. 2013 Apr 1;19(7):1748-59. [Content Brief]

[4]. Kate E Lines, et al. Epigenetic modifiers reduce proliferation of human neuroendocrine tumour cell lines. Endocrine Abstracts (2013) 31 P149

PFI-1 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8786 mL	14.3930 mL	28.7861 mL	71.9652 mL
	5 mM	0.5757 mL	2.8786 mL	5.7572 mL	14.3930 mL
	10 mM	0.2879 mL	1.4393 mL	2.8786 mL	7.1965 mL
	15 mM	0.1919 mL	0.9595 mL	1.9191 mL	4.7977 mL
	20 mM	0.1439 mL	0.7197 mL	1.4393 mL	3.5983 mL
	25 mM	0.1151 mL	0.5757 mL	1.1514 mL	2.8786 mL
	30 mM	0.0960 mL	0.4798 mL	0.9595 mL	2.3988 mL
	40 mM	0.0720 mL	0.3598 mL	0.7197 mL	1.7991 mL
	50 mM	0.0576 mL	0.2879 mL	0.5757 mL	1.4393 mL
	60 mM	0.0480 mL	0.2399 mL	0.4798 mL	1.1994 mL
	80 mM	0.0360 mL	0.1799 mL	0.3598 mL	0.8996 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PFI-11403764-72-6PFI1PFI 1Epigenetic Reader DomainAutophagyApoptosisInhibitorinhibitorinhibit

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