1. Metabolic Enzyme/Protease
  2. Phosphodiesterase (PDE)
  3. Phosphodiesterase I

Phosphodiesterase I  (Synonyms: 磷酸二酯酶 I; PDE)

目录号: HY-P2878
COA 产品使用指南 技术支持

Phosphodiesterase I (PDE) 是一种可以催化环核苷酸 3 '环磷酸键水解的酶,常用于生化研究。Phosphodiesterase I 可作为第二信使分子 cAMP 和 cGMP 介导的信号转导的重要调节因子。根据其对环核苷酸的特异性也可以分别不同类型,如 PDE1-PDE11,在多种疾病方面也具有一定的潜力。

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Phosphodiesterase I Chemical Structure

Phosphodiesterase I Chemical Structure

CAS No. : 9025-82-5

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100 U ¥2800
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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Phosphodiesterase I (PDE) is an enzyme that can catalyze the hydrolysis of the 3' ring phosphate bond of cyclic nucleotides, and is often used in biochemical research. Phosphodiesterase I acts as an important regulator of signal transduction mediated by the second messenger molecules cAMP and cGMP. According to their specificity to cyclic nucleotides, they can also be divided into different types, such as PDE1-PDE11, which also have certain potential in various diseases[1].

体外研究
(In Vitro)

产品信息
磷酸二脂酶 I 是利用菌种发酵,经先进的提取工艺,超滤浓缩、干燥精制而成的酶制剂。
温度范围:有效温度范围:45℃-85℃ , 最适温度范围:65℃-75℃
pH 范围:有效 pH 范围:4.0-8.0, 最适 pH 范围:4.5-5.5
激活剂:Mg2+
抑制剂:还原剂,如谷胱甘肽、半胱氨酸和抗坏血酸,5 mM EDTA 可完全抑制该酶,而 ATP、ADP 和 AMP 可部分抑制该酶

使用说明
可溶于水 (反应 buffer 建议加入 Mg2+),或根据具体实验参考文献溶解 (如使用 0.11 M Tris-HCl buffer, pH 8.9, 0.11 M NaCl 和 15 mM MgCl2 溶解)。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
CAS 号
性状

固体

颜色

White to off-white

中文名称

磷酸二酯酶 I

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Phosphodiesterase I
目录号:
HY-P2878
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