Animal-Free IL-5 蛋白, Mouse (His)

IL-5 protein is expressed by T lymphocytes and NK cells and regulates eosinophils, affecting their survival, differentiation and chemotaxis.In addition, IL-5 stimulates immunoglobulin production, growth, and differentiation of activated and resting B cells.Animal-Free IL-5 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-5 protein, expressed by E.coli , with C-His labeled tag.

IL-5 蛋白是一种同型二聚体细胞因子，主要由 T 淋巴细胞和 NK 细胞表达，通过影响嗜酸性粒细胞的存活、分化和趋化性，在调节嗜酸性粒细胞中发挥关键作用。此外，IL-5 作用于激活的和静止的 B 细胞，刺激免疫球蛋白的产生、生长和分化。从机制上讲，IL-5的生物学效应是通过由IL5RA亚基和细胞因子受体共同亚基β/CSF2RB组成的受体复合物介导的。与受体结合后，IL-5 会触发各种激酶的激活，包括 LYN、SYK 和 JAK2，从而通过 RAS-MAPK 和 JAK-STAT5 途径传播信号（通过相似性）。在结构上，IL-5 形成二硫键连接的同二聚体，并与其受体成分 IL5RA 和 CSF2RB 相互作用，突出了其分子相互作用在协调免疫反应中的特异性和复杂性。

Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant mouse IL-5 is > 5 x 10⁶ IU/mg

Mouse

E. coli

C-His

P04401 (M21-G133)

16191  [NCBI]

MEIPMSTVVKETLTQLSAHRALLTSNETMRLPVPTHKNHQLCIGEIFQGLDILKNQTVRGGTVEMLFQNLSLIKKYIDRQKEKCGEERRRTRQFLDYLQEFLGVMSTEWAMEG

Approximately 13.93 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.