Apolipoprotein A-I/APOA1 蛋白, Human

Apolipoprotein A-I/APOA1 Protein is a secreted protein located on the outside of cell membranes and is the main structural apolipoprotein of high-density lipoprotein (HDL), playing a key role in the reverse cholesterol transport pathway. Apolipoprotein A-I/APOA1 protein interacts with the cell surface receptor SR-BI, thereby promoting dengue virus (DV) infection. Apolipoprotein A-I/APOA1 Protein can also activate sperm motility as part of the SPAP complex. Apolipoprotein A-I/APOA1 Protein has anti-inflammatory, anti-atherosclerotic, anti-apoptotic, anti-tumor, and anti-thrombotic functions. Apolipoprotein A-I/APOA1 Protein Protein, Human is made up of 267 amino acids and is expressed in Escherichia coli (E. coli)^[1][2][3][4].

Apolipoprotein A-I/APOA1 Protein 是人类血浆中最常见的蛋白质之一，其含量通常为 100-150 mg/dl，主要由肝脏 (80%) 和肠道 (20%) 产生^[3]。
Apolipoprotein A-I/APOA1 Protein 的中心区域 (140-170 aa) 主要与 LCAT 的激活相关，而 C 末端结构域区域 (181-243 aa) 在脂质结合中起关键作用^[3]。
Apolipoprotein A-I/APOA1 Protein 通过促进胆固醇从组织流出和充当卵磷脂胆固醇酰基转移酶 (LCAT) 的辅因子，参与胆固醇从组织到肝脏的逆向运输以进行排泄，可有效保护人体免受胆固醇在组织中的积聚^[1]。
Apolipoprotein A-I/APOA1 Protein 和 SRB1 相互作用时，细胞粘附分子受到抑制。这种相互作用将 miRNA223 引入内皮细胞，从而抑制内皮膜上的 ICAM 和 VCAM 表达，并阻止单核细胞粘附在内皮细胞上，从而具有抗炎作用^[3]。
Apolipoprotein A-I/APOA1 Protein 可以桥接 DV 颗粒和细胞受体 SR-BI，并促进 DV 与细胞的附着，从而进入细胞引起病毒感染^[4]。
Apolipoprotein A-I/APOA1 Protein 与病毒感染、动脉粥样硬化、糖尿病、神经系统疾病、肿瘤等疾病有关^[3][4][5][6]。

Apolipoprotein A-I/APOA1 Protein 可以将胆固醇输送到类固醇生成组织，并从外周组织中去除多余游离胆固醇，在血浆中酯化并运输到肝脏排泄^[1]。
Apolipoprotein A-I/APOA1 Protein (1.68 μg/mL, 30 min) 与细胞表面受体 SR-BI 相互作用，从而增强登革热病毒 (DV) 与人肝癌细胞系 Huh-7 的附着并促进病毒感染^[4]。
Apolipoprotein A-I/APOA1 Protein (100 μg/mL, 48 h) 可以抑制 ID8 小鼠上皮癌细胞的细胞活力和增殖^[6]。

Apolipoprotein A-I/APOA1 Protein 在人 Apo A-I 转基因且载脂蛋白 E (Apo E) 基因敲除的小鼠中过表达可以增加高密度脂蛋白并抑制动脉粥样硬化^[5]。
Apolipoprotein A-I/APOA1 Protein 在人 Apo A-I 转基因的卵巢癌小鼠中过表达从而具有抗肿瘤活性^[6]。

Human

E. coli

Tag Free

P02647 (R19-Q267)

335  [NCBI]

RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ

Approximately 25-27 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Obici L, et al. Structure, function and amyloidogenic propensity of apolipoprotein A-I. Amyloid. 2006 Dec;13(4):191-205.  [Content Brief]

  [2]. Akerlöf E, et al. Identification of apolipoprotein A1 and immunoglobulin as components of a serum complex that mediates activation of human sperm motility. Biochemistry. 1991 Sep 17;30(37):8986-90.  [Content Brief]

  [3]. Bhale AS, et al. Leveraging knowledge of HDLs major protein ApoA1: Structure, function, mutations, and potential therapeutics. Biomed Pharmacother. 2022 Oct;154:113634.  [Content Brief]

  [4]. Li Y, et al. Human apolipoprotein A-I is associated with dengue virus and enhances virus infection through SR-BI. PLoS One. 2013 Jul 24;8(7):e70390.  [Content Brief]

  [5]. Plump AS, et al. Human apolipoprotein A-I gene expression increases high density lipoprotein and suppresses atherosclerosis in the apolipoprotein E-deficient mouse. Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9607-11.  [Content Brief]

  [6]. Su F, et al. Apolipoprotein A-I (apoA-I) and apoA-I mimetic peptides inhibit tumor development in a mouse model of ovarian cancer. Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19997-20002.  [Content Brief]