CAMKI alpha/CAMK1 蛋白, Human (HEK293, His)

CAMKI alpha/CAMK1 Protein, Human (HEK293, His), a recombinant human CAMK1 produced in HEK293 cells, has a His tag at the N-terminus. CAMK1 is involved in multiple cell functions, including calmodulin binding, ATP binding, signal transduction, development and cell differentiation^[1].

钙/钙调蛋白依赖性蛋白激酶 (CAMK) 可以控制多种肿瘤类型中的多种癌症相关功能。PPI 网络表明 CALM1、CALM3、CREB1、CALM2、SYN1、NOS3、ATF1、GAPDH、PPM1F 和 FBXL12 是与 CAMK1^[1] 相关的重要基因。

The specific activity was determined to be 120.85 nmol/min/mg that incubate at room temperature for 40min and using 200 μg/mL Autocamtide-2 synthetic peptide (HY-P1528) as substrate.

Human

HEK293

C-6*His

Q14012 (M1-L370)

8536  [NCBI]

MLGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQKLVAIKCIAKEALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGELFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMISDFGLSKMEDPGSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGDTALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTASHGELLTPVAGGPAAGCCCRDCCVEPGTELSPTLPHQLHHHHHH

Approximately 40.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Yangyang Lei, et al. Expression of CAMK1 and its association with clinicopathologic characteristics in pancreatic cancer. J Cell Mol Med. 2021 Jan;25(2):1198-1206.  [Content Brief]