Casein Kinase I alpha 蛋白, Human (sf9, GST)

Casein kinase I α is a member of casein kinases that exhibits a preference for phosphorylation of acidic proteins such as casein. It phosphorylates many substrates, including CTNNB1 at "Ser-45" involved in Wnt signaling. Casein Kinase I alpha Protein, Human (sf9, GST) is the recombinant human-derived Casein Kinase I alpha protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of Casein Kinase I alpha Protein, Human (sf9, GST) is 337 a.a., with molecular weight of ~62 kDa.

酪蛋白激酶 I α 是酪蛋白激酶家族的成员，其特点是偏爱酸性底物，例如酪蛋白。它具有多功能性，可磷酸化多种蛋白质，并通过磷酸化 CTNNB1 的“Ser-45”位点积极参与 Wnt 信号传导。此外，酪蛋白激酶 I α 可能有助于 PER1 和 PER2 的磷酸化，表明其在昼夜节律调节中具有潜在作用。除了参与细胞信号传导途径之外，该激酶可能在有丝分裂过程中的染色体分离中发挥至关重要的作用，并通过影响角蛋白细胞骨架的分解来调节上皮细胞迁移。此外，酪蛋白激酶 I α 作为 mTORC1 和 mTORC2 信号的正调节因子响应营养刺激，介导 DEPTOR 抑制剂的磷酸化。在另一种情况下，它通过 NLRP3 的磷酸化充当 NLRP3 炎性体组装的抑制剂，突出了其在细胞过程中的多种作用。

Human

Sf9 insect cells

N-GST

P48729-1 (M1-F337)

1452  [NCBI]

MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF

Approximately 62 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.