Cathepsin C/DPPI 蛋白, Human (HEK293, C-His)

Cathepsin C/DPPI Protein, a thiol protease, exhibits dipeptidylpeptidase activity with broad substrate specificity, excluding proline at the P1 position and arginine at the P2 position. Operating as both exopeptidase and endopeptidase, it displays catalytic versatility. Crucially, Cathepsin C/DPPI activates serine proteases like elastase, cathepsin G, and granzymes A and B. Cathepsin C/DPPI Protein, Human (HEK293, C-His) is the recombinant human-derived Cathepsin C/DPPI protein, expressed by HEK293, with C-10*His labeled tag.

组织蛋白酶 C/DPPI 蛋白作为硫醇蛋白酶发挥作用，显示出二肽基肽酶活性，具有广泛的底物特异性，涵盖极性和疏水性氨基酸。值得注意的是，脯氨酸被限制在 P1 位上，而精氨酸不能占据底物的 P2 位。该酶既作为外肽酶又作为内肽酶发挥作用，显示出其催化功能的多功能性。重要的是，组织蛋白酶 C/DPPI 在丝氨酸蛋白酶（包括弹性蛋白酶、组织蛋白酶 G 以及颗粒酶 A 和 B）的激活中发挥着至关重要的作用。

Measured by its ability to cleave the fluorogenic peptide substrate 10 μM Gly-Arg-AMC (HY-P4342) that incubate at room temperature in kinetic Mode for 5 minutes. The specific activity is 2567.11 pmol/min/µg.

Human

HEK293

C-10*His

P53634-1/NP_001805.3 (D25-L463)

1075

Full Length of Isoform-1 Mature Protein

DTPANCTYLDLLGTWVFQVGSSGSQRDVNCSVMGPQEKKVVVYLQKLDTAYDDLGNSGHFTIIYNQGFEIVLNDYKWFAFFKYKEEGSKVTTYCNETMTGWVHDVLGRNWACFTGKKVGTASENVYVNTAHLKNSQEKYSNRLYKYDHNFVKAINAIQKSWTATTYMEYETLTLGDMIRRSGGHSRKIPRPKPAPLTAEIQQKILHLPTSWDWRNVHGINFVSPVRNQASCGSCYSFASMGMLEARIRILTNNSQTPILSPQEVVSCSQYAQGCEGGFPYLIAGKYAQDFGLVEEACFPYTGTDSPCKMKEDCFRYYSSEYHYVGGFYGGCNEALMKLELVHHGPMAVAFEVYDDFLHYKKGIYHHTGLRDPFNPFELTNHAVLLVGYGTDSASGMDYWIVKNSWGTGWGENGYFRIRRGTDECAIESIAVAATPIPKL

Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.