DNase1L3 蛋白, Mouse (His)

DNase1L3 protein has DNA hydrolytic activity and can effectively cleave single-stranded and double-stranded DNA to generate 3'-OH terminal fragments. It cleaves chromatin into nucleosomal units and participates in internucleosomal DNA fragmentation during apoptosis and necrosis. DNase1L3 Protein, Mouse (His) is the recombinant mouse-derived DNase1L3 protein, expressed by E. coli , with C-6*His labeled tag. The total length of DNase1L3 Protein, Mouse (His) is 285 a.a., with molecular weight of ~42 kDa.

DNase1L3 蛋白具有 DNA 水解活性，擅长单链和双链 DNA 切割，生成带有 3'-OH 末端的 DNA 片段。它能够将染色质切割成核小体单元，参与细胞凋亡和坏死过程中的核小体间 DNA 断裂 (INDF)。它在细胞凋亡中的作用包括肌源性和神经元分化，以及 BCR 介导的自身反应性 B 细胞的克隆删除。DNase1L3 对凋亡细胞来源的膜包被微粒中的染色质具有活性，可抑制抗 DNA 自身免疫。与 DNASE1 一起，它在降解中性粒细胞胞外陷阱 (NET) 方面发挥着关键作用，NETs 主要由 DNA 纤维组成，在炎症过程中由中性粒细胞释放以结合病原体。DNASE1 和 DNASE1L3 在分解血管内 NET 方面的协同作用对于防止血栓形成至关重要，血栓可能会阻塞血管并导致炎症后的器官损伤。

Mouse

E. coli

C-6*His

O55070 (L26-S310)

13421  [NCBI]

LRLCSFNVRSFGASKKENHEAMDIIVKIIKRCDLILLMEIKDSSNNICPMLMEKLNGNSRRSTTYNYVISSRLGRNTYKEQYAFVYKEKLVSVKTKYHYHDYQDGDTDVFSREPFVVWFHSPFTAVKDFVIVPLHTTPETSVKEIDELVDVYTDVRSQWKTENFIFMGDFNAGCSYVPKKAWQNIRLRTDPKFVWLIGDQEDTTVKKSTSCAYDRIVLCGQEIVNSVVPRSSGVFDFQKAYDLSEEEALDVSDHFPVEFKLQSSRAFTNNRKSVSLKKRKKGNRS

Approximately 42 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6%Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.