GLO1/Glyoxalase I 蛋白, Mouse (His)

GLO1 is an important enzyme responsible for catalyzing the conversion of hemithiol (the product of methylglyoxal and glutathione) to S-lactoylglutathione. This enzyme activity plays a crucial role in cellular detoxification by mitigating the effects of methylglyoxal, a reactive carbonyl species. GLO1/Glyoxalase I Protein, Mouse (His) is the recombinant mouse-derived GLO1/Glyoxalase I protein, expressed by E. coli , with N-His, N-6*His labeled tag. The total length of GLO1/Glyoxalase I Protein, Mouse (His) is 183 a.a., with molecular weight of ~25 & 48 kDa, respectively.

乙二醛酶 I (GLO1)，也称为乳酰谷胱甘肽裂解酶，是参与细胞解毒过程的关键酶。GLO1 催化半硫醇（一种由甲基乙二醛和谷胱甘肽反应形成的化合物）转化为 S-乳酰谷胱甘肽。这种酶活性对于清除有毒的甲基乙二醛（一种反应性二羰基化合物）并防止其对细胞成分产生有害影响至关重要。此外，GLO1 在调节 NF-kappa-B 转录活性中发挥作用，可能影响细胞对炎症的反应。此外，GLO1 是正常破骨细胞生成所必需的，这表明它在骨骼发育和重塑中具有重要意义。GLO1 的多方面功能强调了其在细胞稳态中的重要性及其对各种生理过程的潜在参与。

Measured by its ability to catalyze the formation of S-D-lactoylglutathione from the hemimercaptal adduct that forms spontaneously between methylglyoxal and reduced glutathione. The specific activity is 100.39 nmol/min/μg, as measured under the described conditions.

Mouse

E. coli

N-6*His

Q9CPU0/NP_079650.3 (A2-I184)

109801  [NCBI]

AEPQPASSGLTDETAFSCCSDPDPSTKDFLLQQTMLRIKDPKKSLDFYTRVLGLTLLQKLDFPAMKFSLYFLAYEDKNDIPKDKSEKTAWTFSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKIATII

Approximately 25&48 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.