I-309/CCL1 蛋白, Human (CHO)

I-309/CCL1 Protein, Human (CHO) is a cytokine that mediates inflammatory immune responses, viral infections, and tumorigenesis by interacting with the CCR8 chemokine receptor on the cell surface and attracting monocytes, natural killer cells, and immature B-cell nuclear dendritic cells. I-309/CCL1 Protein, Human (CHO) is a recombinant human CCL1 (K24-K96) expressed by CHO^[1].

CCL1 是一种小糖蛋白，属于 CC 趋化因子家族，分子量约为 15-16 kDa，在人类中称为小诱导细胞因子 I-309，在小鼠中称为 TCA-3。CCL1 由活化的单核细胞、巨噬细胞、T 淋巴细胞和内皮细胞分泌，对单核细胞有趋化作用，但对中性粒细胞没有趋化作用^[1]。CCL1 可通过与细胞表面趋化因子受体 CCR8 相互作用而被激活，从而诱导 Ca²⁺ 内流，刺激单核细胞中细胞质游离钙浓度的短暂增加，并通过 RAS/MAPK 抑制胸腺细胞系的凋亡途径。其中，CCR8 在单核细胞、巨噬细胞、Th2 和调节性 T 淋巴细胞中组成型表达，是人类 CCL1 和病毒趋化因子 vCCL1（病毒巨噬细胞炎症蛋白 1）的唯一受体。CCR8 已被证明与多发性硬化症病变中的吞噬巨噬细胞和激活的小胶质细胞相关，并且与脱髓鞘活动直接相关^[2]。CCL1 通过白细胞募集参与炎症过程，并在血管生成以及其他病毒和肿瘤过程中发挥关键作用。CCL1 基因中的许多单核苷酸多态性 (SNP) 与慢性阻塞性肺病 (COPD) 的进展有关。与此同时，CCL1 在各种 CNS 功能和疾病中发挥作用，并可能与神经炎症性疾病^[3]相关。

CCL1 (100 ng/mL, 3h) stimulates a 14-fold increase in human aortic smooth muscle cells (VSMCs) migration and induces VSMC chemotaxis. Also, it stimulates VSMC secretion of pro - MMP2 and induces MMP-2 mRNA^[4].

1.The ED₅₀ is <1 μg/mL as measured by CHO-K1/Gα15/hCCR8 cells (human Gα15 and human CCR8 stably expressed in CHO-K1 cells).
2.Determined by its ability to chemoattract THP-1 cells. The ED₅₀ for this effect is 22 ng/mL.

Human

CHO

Tag Free

P22362 (K24-K96)

6346  [NCBI]

KSMQVPFSRCCFSFAEQEIPLRAILCYRNTSSICSNEGLIFKLKRGKEACALDTVGWVQRHRKMLRHCPSKRK

Approximately 15 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS or PBS, pH 7.4, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. M D Miller, et al. The human cytokine I-309 is a monocyte chemoattractant. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2950-4.  [Content Brief]

  [2]. Miller MD, et al. The human cytokine I-309 is a monocyte chemoattractant. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2950-4.  [Content Brief]

  [3]. A Zingoni, et al. The chemokine receptor CCR8 is preferentially expressed in Th2 but not Th1 cells. J Immunol. 1998 Jul 15;161(2):547-51.  [Content Brief]

  [4]. Roos RS, et al. Identification of CCR8, the receptor for the human CC chemokine I-309. J Biol Chem. 1997 Jul 11;272(28):17251-4.  [Content Brief]

  [5]. Gültekin Tamgüney, et al. Autocrine stimulation of rhadinovirus-transformed T cells by the chemokine CCL1/I-309. Oncogene. 2004 Nov 4;23(52):8475-85.  [Content Brief]

  [6]. Nasreen S Haque, et al. Chemokine receptor-8 (CCR8) mediates human vascular smooth muscle cell chemotaxis and metalloproteinase-2 secretion. Blood. 2004 Feb 15;103(4):1296-304.  [Content Brief]