I-309/CCL1 蛋白, Mouse (HEK293, Fc)

I-309/CCL1 Protein, Mouse (HEK293, Fc) is a cytokine that mediates inflammatory immune responses, viral infections, and tumorigenesis by interacting with the CCR8 chemokine receptor on the cell surface and attracting monocytes, natural killer cells, and immature B-cell nuclear dendritic cells. I-309/CCL1 Protein, Mouse (HEK293, Fc) is a recombinant mouse CCL1 (K24-C92) expressed by HEK293 with a -hFc tag at the nitrogen end^[1].

CCL1 是一种小糖蛋白，属于 CC 趋化因子家族，分子量约为 15-16 kDa，在人类中称为小诱导细胞因子 I-309，在小鼠中称为 TCA-3。CCL1 由活化的单核细胞、巨噬细胞、T 淋巴细胞和内皮细胞分泌，对单核细胞有趋化作用，但对中性粒细胞没有趋化作用^[1]。CCL1 可通过与细胞表面趋化因子受体 CCR8 相互作用而被激活，从而诱导 Ca²⁺ 内流，刺激单核细胞中细胞质游离钙浓度的短暂增加，并通过 RAS/MAPK 抑制胸腺细胞系的凋亡途径。其中，CCR8 在单核细胞、巨噬细胞、Th2 和调节性 T 淋巴细胞中组成型表达，是人类 CCL1 和病毒趋化因子 vCCL1（病毒巨噬细胞炎症蛋白 1）的唯一受体。CCR8 已被证明与多发性硬化症病变中的吞噬巨噬细胞和激活的小胶质细胞相关，并且与脱髓鞘活动直接相关^[2]。CCL1 通过白细胞募集参与炎症过程，并在血管生成以及其他病毒和肿瘤过程中发挥关键作用。CCL1 基因中的许多单核苷酸多态性 (SNP) 与慢性阻塞性肺病 (COPD) 的进展有关。与此同时，CCL1 在各种 CNS 功能和疾病中发挥作用，并可能与神经炎症性疾病^[3]相关。

CCL1 (instill, in 5 μL of LPS-free PBS (5 μg/kg), every 3 days for one month ) can induce pulmonary fibrotic changes, decrease lung function and increase collagen deposition in C57BL/6J mice by exogenous addition. Besides, CCL1 activates fibroblasts in the lung through CCR8 recruitment and induces migration of lung fibroblasts^[4].
  CCL1 (s.c., 1-1 µg/kg) produces a dose-dependent and long-lasting increase in thermal withdrawal latency, noxious thermal stimulation is observed at 1 µg/kg and a decrease in spinal cord neurons expressing Fos protein, further indicating its analgesic properties in male Swiss mice^[5].

Mouse

HEK293

N-hFc

P10146 (K24-C92)

20290  [NCBI]

Approximately 44 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. M D Miller, et al. The human cytokine I-309 is a monocyte chemoattractant. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2950-4.  [Content Brief]

  [2]. A Zingoni, et al. The chemokine receptor CCR8 is preferentially expressed in Th2 but not Th1 cells. J Immunol. 1998 Jul 15;161(2):547-51.  [Content Brief]

  [3]. Gültekin Tamgüney, et al. Autocrine stimulation of rhadinovirus-transformed T cells by the chemokine CCL1/I-309. Oncogene. 2004 Nov 4;23(52):8475-85.  [Content Brief]

  [4]. Shan-Shan Liu, et al. The chemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis. Immunity. 2021 Oct 12;54(10):2433-2435.  [Content Brief]

  [5]. Mario García-Domínguez, et al. The Systemic Administration of the Chemokine CCL1 Evokes Thermal Analgesia in Mice Through the Activation of the Endocannabinoid System. Cell Mol Neurobiol. 2019 Nov;39(8):1115-1124.  [Content Brief]