1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-gamma Receptor
  5. IFN-gamma R1
  6. IFN-gamma R1/CD119 蛋白, Mouse (HEK293, His)

IFN-gamma R1/CD119 蛋白, Mouse (HEK293, His)

目录号: HY-P72611
COA 产品使用指南

IFN-gamma R1,IFN-gamma 受体的亚基之一,是 IFN-γ 的结合受体。IFN-gamma R1 可以与 IFN-gamma R2 结合形成功能性受体。与配体 IFN-γ 结合后,IFN-gamma R1IFN-gamma R2 寡聚化并发生磷酸化。然后,JAK1JAK2 发生磷酸化并激活,STAT1 被募集到受体复合物附近。磷酸化的 STAT1 易位至细胞核,从而调节 IFN-反应性的基因 (例如 CD54) 的表达。IFN-gamma R1/CD119 蛋白, Mouse (HEK293, His) 是一种由 HEK293 细胞产生的重组小鼠 IFN-gamma R1 ,C 端带有 6*His 标签。

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规格 价格 是否有货 数量
10 μg ¥400 In-stock
50 μg ¥1150 询价
100 μg   询价  

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描述

IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 forms the functional receptor with IFN-gamma R2. Upon binding with IFN-gamma, IFN-gamma R1 and IFN-gamma R2 oligomerize and transphosphorylate[1]. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)[2]. IFN-gamma R1/CD119 Protein, Mouse (HEK293, His) is a recombinant mouse IFN-gamma R1 with C-terminal 6*His tag, which is produced in HEK293 cells.

研究背景

IFN-γ R1 (CD119) 是 IFN-γ 受体的亚基之一,是 IFN-γ 的受体。IFN-γ R1 组成型表达于几乎所有细胞的表面[1]
IFN-γ R1 可以与 IFN-γ R2 结合形成功能性受体。与 IFN-γ 结合后,IFNγR1 和 IFNγR2 寡聚并转磷酸化[1]。然后,JAK1JAK2 被磷酸化并激活,STAT1 被招募到受体复合物中。IFNγR1 的磷酸化为 STAT1 创建了一个对接位点,并导致 STAT1 磷酸化。磷酸化的 STAT1 易位到细胞核,在那里调节 IFN 反应基因(例如 CD54)的表达。IFN-γ R1 缺陷与 IFN-γ 介导的免疫反应以及感染易感性增加有关。IFN-gamma R1 信号通路对于激活癌细胞死亡和抑制癌症进展具有重要作用[3]
小鼠 IFN-gamma R1 由胞外结构域 (A26-S254)、螺旋结构域 (I255) 组成-Y275)和胞质结构域(W276-S477)。IFNAR1的氨基酸序列在不同物种中有所不同。小鼠 IFN-γ R1 与人类具有 50% 的氨基酸序列同一性。IFN-gamma R1 在抗菌、抗病毒和抗肿瘤反应中发挥着关键作用[2]

体外研究
(In Vitro)

IFN-gamma R1 (murine, 0-20 μg/mL, 4 h) suppresses AP(+)-exosome-mediated NO synthesis in RAW264.7 cells[4].

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

P15261 (A26-D253)

基因 ID
中文名
重组小鼠 IFN-gamma R1/CD119 蛋白 (228a.a, HEK293, His)
同用名
Interferon gamma receptor 1; IFN-gamma-R1; IFN-gamma-R-alpha; CD119; Ifngr1
氨基酸序列

ALTSTEDPEPPSVPVPTNVLIKSYNLNPVVCWEYQNMSQTPIFTVQVKVYSGSWTDSCTNISDHCCNIYEQIMYPDVSAWARVKAKVGQKESDYARSKEFLMCLKGKVGPPGLEIRRKKEEQLSVLVFHPEVVVNGESQGTMFGDGSTCYTFDYTVYVEHNRSGEILHTKHTVEKEECNETLCELNISVSTLDSRYCISVDGISSFWQVRTEKSKDVCIPPFHDDRKD

分子量

38-55 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IFN-gamma R1/CD119 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IFN-gamma R1/CD119 Protein, Mouse (HEK293, His)
目录号:
HY-P72611
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