LR3 IGF-I/IGF-1 蛋白, Human (83a.a, E51R)

The LR3 IGF-I/IGF-1 protein is similar to insulin and has potent growth-promoting activity that exceeds the efficacy of insulin. As a potential physiological regulator, it stimulates glucose transport and glycogen synthesis in osteoblasts even at significantly lower concentrations than insulin. LR3 IGF-I/IGF-1 Protein, Human (83a.a, E51R) is the recombinant human-derived LR3 IGF-I/IGF-1 protein, expressed by E. coli , with tag free. and E51R, , , , mutation. The total length of LR3 IGF-I/IGF-1 Protein, Human (83a.a, E51R) is 70 a.a., with molecular weight of ~11.0 kDa.

LR3 IGF-I/IGF-1 蛋白在结构和功能上类似于胰岛素，与同类蛋白相比，其促生长活性显着增强。LR3 IGF-I 定位为潜在的生理调节剂，可以控制成骨细胞中的 [1-14C]-2-脱氧-D-葡萄糖 (2DG) 转运和糖原合成，证明可以有效刺激骨源性成骨细胞 (PyMS) 中的葡萄糖转运即使浓度明显低于胰岛素，细胞也能发挥作用。其多方面的作用延伸至潜在参与突触成熟和Ca(2+)依赖性胞吐作用，这对于嗅球中的气味感知至关重要。作为 IGF1R 的配体，LR3 IGF-I 与 α 亚基结合，启动内在酪氨酸激酶活性的激活，使 β 亚基中的酪氨酸残基自磷酸化。这种激活会触发一系列下游信号传导事件，从而激活 PI3K-AKT/PKB 和 Ras-MAPK 通路。此外，LR3 IGF-I 与整联蛋白（ITGAV:ITGB3 和 ITGA6:ITGB4）和 IGFR1 形成关键的三元复合物，这对于全面的 IGF1 信号传导至关重要，影响 IGFR1、MAPK3/ERK1、MAPK1/ERK2 和 AKT1 的磷酸化和激活。它还表现出多种分子相互作用，包括与 SH2D3C 同工型 2 的相互作用。

Measured in a cell proliferation assay using MCF-7 cells. The ED₅₀ for this effect is 0.38-2.174 ng/mL.

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  Measured in a cell proliferation assay using MCF-7 cells. The ED₅₀ for this effect is 0.3840 ng/mL, corresponding to a specific activity is 2.60×10⁶ units/mg.

Human

E. coli

Tag Free

P05019-1 (G49-A118, E51R, with a 13 amino acid extension peptide at the N terminal)

3479  [NCBI]

MFPAMPLSSLFVNGPRTLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA

Approximately 9-11.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAc-HAc, 4% Mannitol, pH 4.5 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.