IGF-I R 蛋白, Rat (HEK293, His)

Igf1r Protein, a cell surface receptor, is involved in growth regulation and development. Dysregulation of Igf1r Protein has been linked to diseases like cancer and growth disorders. Targeting Igf1r Protein may offer potential therapeutic interventions in these conditions by modulating cell growth, inhibiting tumor progression, and managing growth-related disorders. IGF-I R Protein, Rat (HEK293, His) is the recombinant rat-derived IGF-I R protein, expressed by HEK293 , with C-His labeled tag.

IGF1R 是一种受体蛋白，在细胞生长和生存控制中发挥着至关重要的作用。它参与肿瘤转化和恶性细胞的存活。当配体（例如胰岛素样生长因子 1 (IGF1)）与 IGF1R 结合时，它会激活受体激酶，导致受体自身磷酸化以及多种信号转接蛋白（包括胰岛素受体底物 (IRS1/2)）的磷酸化、Shc 和 14-3-3 蛋白质。IRS 蛋白的磷酸化激活两个主要信号传导途径：PI3K-AKT/PKB 途径和 Ras-MAPK 途径。MAPK 途径的激活会导致细胞增殖增加，而 PI3K 途径的激活会抑制细胞凋亡并刺激蛋白质合成。磷酸化的 IRS1 可以激活 PI3K (PIK3R1) 的调节亚基，然后激活下游底物，包括蛋白 AKT/PKB。AKT 磷酸化通过 mTOR 激活增强蛋白质合成，并通过磷酸化和灭活 BAD 触发 IGF1R 的抗凋亡作用。除了 PI3K-AKT/PKB 和 Ras-MAPK 途径外，IGF1R 还通过 Janus 激酶/信号转导器和转录途径激活剂 (JAK/STAT) 发出信号。JAK 蛋白的磷酸化导致信号转导子和转录激活子 (STAT) 蛋白的磷酸化和激活。特别是 STAT3 的激活对于 IGF1R 的转化活性可能至关重要。JAK/STAT 途径激活基因转录并可能有助于 IGF1R 的转化活性。IGF1R 还可以激活 JNK 激酶。然而，IGF1通过磷酸化和抑制MAP3K5/ASK1来对JNK激活发挥抑制作用，MAP3K5/ASK1可直接与IGF1R结合。此外，当 IGF1R 与胰岛素受体 (INSR) 形成杂合受体时，它可以结合 IGF1。总体而言，IGF1R 通过多种信号通路（包括 PI3K-AKT/PKB、Ras-MAPK、JAK/STAT 和 JNK 通路）在调节细胞生长、存活和转化中发挥关键作用。

Measured by its binding ability in a functional ELISA. When Rat IGF-I R is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Human IGF-I(HY-P7018). The ED₅₀ for this effect is 24.9 ng/mL.

Rat

HEK293

C-10*His

P24062 (E31-H936)

25718  [NCBI]

EICGPGIDIRNDYQQLKRLENCTVIEGFLHILLISKAEDYRSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTIDWSLILDAVSNNYIVGNKPPKECGDLCPGTLEEKPMCEKTTINNEYNYRCWTTNRCQKMCPSVCGKRACTENNECCHPECLGSCHTPDDNTTCVACRHYYYKGVCVPACPPGTYRFEGWRCVDRDFCANIPNAESSDSDGFVIHDGECMQECPSGFIRNSTQSMYCIPCEGPCPKVCGDEEKKTKTIDSVTSAQMLQGCTILKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRLILGEEQLEGNYSFYVLDNQNLQQLWDWNHRNLTVRSGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLRFTSTTTWKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKEGEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPTLPNGNLSYYIVRWQRQPQDGYLFRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGDKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNSIFVPRPERRRRDVLQVANTTMSSRSRNTTVADTYNITDPEEFETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVPAKTTYENFMH

45-43 kDa, 100-130 kDa&140-180 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.