IL-10 蛋白, Human (HEK293, His)

IL-10 protein is a major immunoregulatory cytokine with profound anti-inflammatory functions that limits excessive tissue destruction caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptors IL10RA and IL10RB, leading to JAK1- and STAT2-mediated phosphorylation of STAT3, which translocates to the nucleus and drives the expression of anti-inflammatory mediators. IL-10 Protein, Human (HEK293, His) is the recombinant human-derived IL-10 protein, expressed by HEK293 , with N-His labeled tag.

IL-10 蛋白是一种关键的免疫调节细胞因子，具有有效的抗炎作用，可防止炎症引起的过度组织损伤。它与由 IL10RA 和 IL10RB 组成的异四聚体受体结合，触发 JAK1 和 STAT2 介导的 STAT3 磷酸化。这导致 STAT3 易位到细胞核中，驱动抗炎介质的表达。IL-10 蛋白靶向巨噬细胞和单核细胞等抗原呈递细胞，抑制促炎细胞因子的释放。它还通过减少 MHC II 类和共刺激分子的表达来阻碍抗原呈递，从而抑制 T 细胞活化。此外，它还能重新编程代谢途径，包括 mTOR 信号传导，以控制巨噬细胞的炎症反应。该蛋白形成同二聚体并与 IL10RA 和 IL10RB 相互作用。

1.Immobilized human IL10-His at 10 μg/mL (100 μl/well) can bind Cynomolgus IL10RA-Fc and the EC₅₀ is 0.31-0.71 μg/mL.
2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells and the ED₅₀ is typically 5-20 ng/mL.

Human

HEK293

N-His

P22301/NP_000563.1 (S19-N178)

3586  [NCBI]

Approximately 20.9 kDa

Greater than 85% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.