IL-12 蛋白, Mouse (CHO)

IL-12 Protein, Mouse (CHO) has both T cell growth and IFN-γ stimulatory properties.

IL-12 参与幼稚 T 细胞向 Th1 细胞的分化。它被称为T细胞刺激因子，可以刺激T细胞的生长和功能。它刺激 T 细胞和自然杀伤 (NK) 细胞产生干扰素-γ (IFN-γ) 和肿瘤坏死因子-α (TNF-α)，并减少 IL-4 介导的 IFN-γ 抑制。IL-12 还具有抗血管生成活性，这意味着它可以阻止新血管的形成^[1][2]。

The ED₅₀ is <0.15 ng/mL as measured by 2D6 cells.

Mouse

CHO

Tag Free

P43431 (R23-A215) & P43432 (M23-S335)

16159  [NCBI]&16160  [NCBI]

p35:
RVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA
p40:
MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

27-29 kDa (p35) and 45-50 kDa (p40)

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hsieh CS, et al. Development of TH1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages. Science. 1993 Apr 23;260(5107):547-9.  [Content Brief]

  [2]. Heinzel FP, et al. Recombinant interleukin 12 cures mice infected with Leishmania major. J Exp Med. 1993 May 1;177(5):1505-9.  [Content Brief]