IL-12 蛋白, Mouse (HEK293)

IL-12 protein is a immune-suppressive heterodimeric cytokine, composed by IL-12A subunit (IL-12p35) and IL-12B subunit (IL-12p40), is naturally produced by dendritic cells^[1]. IL-12 exerts functions to activate and link the innate and acquired immune responses^[2]. IL-12 Protein, Mouse is produced in HEK293 cells, with total length of 506 amino acids and tag free.

IL-12 主要由 B 细胞和树突细胞 (DC) 等专业抗原呈递细胞 (APC) 以及巨噬细胞和粒细胞产生，并调节 T 细胞和自然杀伤细胞反应^[2]。
IL-12 属于白细胞介素 12 家族，是唯一的异二聚体细胞因子，包括 IL-12、IL-23、IL-27 和 IL-35^[3]。
IL-12 通过结合由 IL12R1 和 IL12R2 亚基组成的受体发挥作用，导致包括 JAK 家族激酶 TYK2 和 JAK2 在内的多种细胞底物快速酪氨酸磷酸化^[4]。
IL-12 是幼稚 T 细胞分化为分泌干扰素 γ 的辅助性 T 1 型 CD4+ 淋巴细胞的重要因子^[5]。
IL-12 诱导产生干扰素-γ (IFN-gamma)，有利于辅助性 T 细胞 1 (Th1) 细胞的分化，是先天抵抗和适应性免疫之间的重要联系^[2]。

Measured in a cell proliferation assay using CTLL-2 cells. The ED₅₀ for this effect is 0.004776 ng/mL, corresponding to a specific activity is 2.09×10⁸ units/mg.

• 
  Measured in a cell proliferation assay using CTLL-2 cells. The ED₅₀ for this effect is 0.004776 ng/mL, corresponding to a specific activity is 2.09×10₈ units/mg.

Mouse

HEK293

Tag Free

P43431 (R23-A215) & P43432 (M23-S335)

16159  [NCBI]&16160  [NCBI]

RVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA
&:
MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

20-25&38-45 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Kaliński P, et al. IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T helper cells. J Immunol. 1997 Jul 1;159(1):28-35.  [Content Brief]

  [2]. Wolf SF, et al. Interleukin 12: a key modulator of immune function. Stem Cells. 1994 Mar;12(2):154-68.  [Content Brief]

  [3]. Mattner F, et al. Genetically resistant mice lacking interleukin-12 are susceptible to infection with Leishmania major and mount a polarized Th2 cell response. Eur J Immunol. 1996 Jul;26(7):1553-9.  [Content Brief]

  [4]. Vignali DA, et al. IL-12 family cytokines: immunological playmakers. Nat Immunol. 2012 Jul 19;13(8):722-8.  [Content Brief]

  [5]. Bacon CM, et al. Interleukin 12 (IL-12) induces tyrosine phosphorylation of JAK2 and TYK2: differential use of Janus family tyrosine kinases by IL-2 and IL-12. J Exp Med. 1995 Jan 1;181(1):399-404.  [Content Brief]

  [6]. Cua DJ, et al. Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain. Nature. 2003 Feb 13;421(6924):744-8.  [Content Brief]