1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-5
  5. IL-5 蛋白, Cynomolgus (sf9, His)

IL-5 蛋白, Cynomolgus (sf9, His)

目录号: HY-P73878
产品使用指南

IL-5 蛋白由 T 淋巴细胞和 NK 细胞产生,对于嗜酸性粒细胞的存活、分化和趋化性至关重要。它刺激 B 细胞中免疫球蛋白的产生和生长。IL-5 蛋白通过受体(IL5RA 和 CSF2RB 亚基)激活激酶(LYN、SYK 和 JAK2),通过 RAS-MAPK 和 JAK-STAT5 途径传播信号。它以二硫键连接的同二聚体形式存在。IL-5 蛋白, Cynomolgus (sf9, His) 是重组的 IL-5 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

IL-5 Protein, produced by T-lymphocytes and NK cells, is crucial for eosinophil survival, differentiation, and chemotaxis. It stimulates immunoglobulin production and growth in B-cells. IL-5 Protein activates kinases (LYN, SYK, and JAK2) through a receptor (IL5RA and CSF2RB subunits), propagating signals via RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer. IL-5 Protein, Cynomolgus (sf9, His) is the recombinant cynomolgus-derived IL-5 protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

IL-5 蛋白是一种同型二聚体细胞因子,主要由 T 淋巴细胞和 NK 细胞产生,在嗜酸性粒细胞的存活、分化和趋化性中发挥着至关重要的作用。它还作用于 B 细胞,刺激免疫球蛋白的产生、生长和分化。IL-5蛋白通过由IL5RA和CSF2RB亚基组成的受体发挥作用,导致LYN、SYK和JAK2等多种激酶的激活。这种激活通过 RAS-MAPK 和 JAK-STAT5 途径传播信号。IL-5 蛋白以二硫键连接的同二聚体形式存在。

种属

Cynomolgus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

A0A2K5U1E7 (I20-S134)

基因 ID

/

蛋白结构
N-term
IL-5 (I20-S134)
Accession # A0A2K5U1E7
His
C-term
同用名
Interleukin-5; IL-5; EDF; Eo-CSF; TRF
分子量

Approximately 14.6 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IL-5 Protein, Cynomolgus (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-5 Protein, Cynomolgus (sf9, His)
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