MMP-16 蛋白, Human (I152N, His)

The MMP-16 protein functions as an endopeptidase capable of degrading various components of the extracellular matrix, particularly type III collagen and fibronectin. Its effects extend to the activation of progelatinase A, which contributes to the dynamic remodeling of the extracellular matrix within blood vessels. MMP-16 Protein, Human (I152N, His) is the recombinant human-derived MMP-16 protein, expressed by E. coli , with C-10*His labeled tag. The total length of MMP-16 Protein, Human (I152N, His) is 260 a.a, with molecular weight of ~29  kDa.

MMP-16（基质金属蛋白酶-16）是一种内肽酶，在多种细胞外基质成分（包括 III 型胶原蛋白和纤连蛋白）的降解中发挥着关键作用。它展示了激活原明胶酶 A 的能力，有助于调节血管基质重塑。MMP-16 的短亚型因其对纤连蛋白和 III 型胶原蛋白的裂解而特别引人注目，尽管裂解速度有所降低，但对 I、II、IV 和 V 型胶原蛋白没有明显影响。然而，在 CSPG4 存在的情况下，MMP-16可能在黑色素瘤细胞降解和侵袭I型胶原中发挥作用。这种双重功能强调了 MMP-16 参与细胞外基质动力学的复杂性和环境依赖性，强调了其在生理和病理过程中的潜在影响，包括与血管重塑和癌症进展相关的过程。

Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is >250 pmol/min/µg.

Human

E. coli

C-10*His

P51512 (A32-G291, I152N)

4325  [NCBI]

ATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRKANRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYG

Approximately 29 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, 0.4% SKL, 20% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.