2. 重组蛋白
  3. Immune Checkpoint Proteins CD Antigens
  4. Inhibitory Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins
  5. PD-1
  6. PD-1 蛋白, Human (HEK293, Fc)

PD-1 蛋白, Human (HEK293, Fc)

目录号: HY-P73344
COA 产品使用指南

PD-1蛋白是T细胞上的抑制性受体，通过与CD274/PDCD1L1和CD273/PDCD1LG2结合来维持免疫耐受。它通过与 CD3-TCR 结合并招募 PTPN11/SHP-2 使关键信号分子去磷酸化来阻止 T 细胞活化。PD-1 蛋白, Human (HEK293, Fc) 是重组的 PD-1 蛋白，由 HEK293 表达，带有 C-hFc 标签。PD-1 蛋白, Human (HEK293, Fc) 全长 143 个氨基酸，分子量为 55-66 kDa。

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SPR 检测服务   重组蛋白定制服务

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3.  试用装只面向终端客户。

规格 价格 是否有货 数量
5 μg ￥420 In-stock
10 μg ￥720 In-stock
50 μg ￥2000 In-stock
100 μg   询价  

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描述

PD-1 protein is an inhibitory receptor on T cells that maintains immune tolerance by binding to CD274/PDCD1L1 and CD273/PDCD1LG2. It blocks T cell activation by binding to CD3-TCR and recruiting PTPN11/SHP-2 to dephosphorylate key signaling molecules. PD-1 Protein, Human (HEK293, Fc) is the recombinant human-derived PD-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of PD-1 Protein, Human (HEK293, Fc) is 143 a.a., with molecular weight of 55-66 kDa.
研究背景

PD-1蛋白作为抗原激活T细胞的抑制性受体，在诱导和维持自身免疫耐受中发挥着至关重要的作用。PD-1 与其配体 CD274/PDCD1L1 和 CD273/PDCD1LG2 结合后，会传递抑制信号并与免疫突触中的 CD3-TCR 结合，直接阻碍 T 细胞激活。这种抑制作用通过招募 PTPN11/SHP-2 进一步执行，导致关键 TCR 近端信号分子去磷酸化。PD-1 与 CD274/PDCD1L1 的相互作用会被肿瘤利用来减弱抗肿瘤免疫力，从而抑制细胞毒性 T 淋巴细胞 (CTL) 效应器功能。阻断 PD-1 介导的通路有望逆转耗尽的 T 细胞表型并使抗肿瘤反应正常化，从而为癌症免疫治疗提供了理论依据。
生物活性

1.Immobilized PD-1 Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind PD-L2 Protein, Human, Recombinant (ECD, His Tag), the EC50 is 45-280ng/mL.
2.Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) at 2 μg/mL (100 μl/well) can bind Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated , the EC50 is 25-75 ng/mL.
3. Labeled biotin to PD-1 Protein, Human, Recombinant (Fc Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-1 Protein, Human, Recombinant (Fc Tag), Biotinylated bound to Atezolizumab was 0.3 nM.
种属

Human
表达系统

HEK293
标签

C-hFc
蛋白编号

Q15116 (L25-Q167)
基因 ID
蛋白结构
N-term
PD-1 (L25-Q167)
Accession # Q15116
hFc
C-term
同用名
CD279; hPD-1; PDCD1; Programmed cell death 1; SLEB2
分子量

55-66 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液
组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (212 KB)

产品使用指南 (1213 KB)

PD-1 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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