Podoplanin 蛋白, Rat (HEK293, hFc)

The Podoplanin protein coordinates migration and adhesion by interacting with partners such as CLEC1B and CD9. It aids in blood vessel separation, platelet activation and platelet aggregation. Podoplanin Protein, Rat (HEK293, hFc) is the recombinant rat-derived Podoplanin protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Podoplanin Protein, Rat (HEK293, hFc) is 113 a.a., with molecular weight of ~38.6 kDa.

Podoplanin 是一种多面蛋白质，通过与各种伙伴的相互作用协调与迁移和粘附相关的多种细胞功能。在发育过程中，它通过结合CLEC1B、激活血小板、诱导血小板活化或聚集，有助于血管和淋巴管的分离。相反，它与 CD9 的相互作用减弱了 Podoplanin 诱导的血小板聚集和肺转移。此外，Podoplanin 通过促进 ERZ 磷酸化并通过与 MSN 或 EZR 相互作用触发 RHOA 激活，从而在上皮间质转化 (EMT) 中发挥至关重要的作用，从而导致细胞迁移和侵袭性增加。它与 CD44 的结合促进上皮细胞和肿瘤细胞的定向细胞迁移。在淋巴结内，Podoplanin 通过维持 ERM 蛋白（EZR、MSN 和 RDX）和 MYL9 激活来控制成纤维网状细胞 (FRC) 与细胞外基质 (ECM) 的粘附和肌动球蛋白的收缩。与 CLEC1B 的结合通过阻断侧膜相互作用来促进 FRC 松弛。此外，Podoplanin 通过与 LGALS8 结合参与淋巴内皮与 ECM 的连接。在角质形成细胞中，它会引起形态变化，包括形状拉长和运动性增加。平足蛋白还调节肿瘤细胞中侵袭伪足的稳定性和成熟，影响细胞外基质的降解。此外，它对于出生时正常的肺细胞增殖和肺泡形成至关重要。Podoplanin 表现出同二聚体结构，并与多种蛋白质相互作用，包括 CLEC1B、CD9、LGALS8、HSPA9、CD44、MSN、EZR 和 CCL21，在介导多种细胞功能中发挥着关键作用。

Measured by its binding ability in a functional ELISA. Immobilized Rat Podoplanin at 10 μg/mL (100 μL/well) can bind biotinylated Human CLEC2B. The ED₅₀ for this effect is 0.4651 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Rat Podoplanin at 10 μg/mL (100 μL/well) can bind biotinylated Human CLEC2B. The ED₅₀ for this effect is 0.4651 μg/mL.

Rat

HEK293

C-hFc

Q64294 (G23-L135)

54320  [NCBI]

GAIGALEDDLVTPGPGDDMVNPGLEDRIETTDTTGELDKSTAKAPLVPTQPPIEELPTSGTSDHDHKEHESTTTVKAVTSHSTDKKTTHPNRDNAGGETQTTDKKDGLAVVTL

Approximately 55-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.