Semaphorin-3A/SEMA3A 蛋白, Human (HEK293, N-His)

Semaphorin-3A/SEMA3A proteins are critical for shaping the olfactory system and influence puberty-related neuronal control. SEMA3A is known for inducing neuronal growth cone collapse, which leads to the formation of complex neuronal circuits by guiding the growth cone through a motor inhibitory mechanism. Semaphorin-3A/SEMA3A Protein, Human (HEK293, N-His) is the recombinant human-derived Semaphorin-3A/SEMA3A protein, expressed by HEK293 , with N-6*His labeled tag. The total length of Semaphorin-3A/SEMA3A Protein, Human (HEK293, N-His) is 521 a.a., with molecular weight of approximately 60.52 kDa.

Semaphorin-3A/SEMA3A 蛋白在嗅觉系统的发育中起着至关重要的作用，并对青春期的神经元控制产生影响。SEMA3A 以其诱导神经元生长锥塌陷和麻痹的能力而闻名，它通过运动抑制机制引导特定的生长锥，从而促进神经元回路形成的复杂性。该蛋白通过与复杂的 Neuropilin-1/plexin-1 结合而充当配体，强调其参与转导对神经元引导至关重要的信号线索。此外，SEMA3A 与 PLXND1 相互作用，进一步凸显了其在嗅觉系统发育和青春期相关神经元功能调节的复杂过程中介导细胞相互作用的多方面作用。

Human

HEK293

N-6*His

Q14563 (K26-F546)

10371  [NCBI]

KNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPICTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRPIVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYF

approximately 60.52 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.