Siglec-2/CD22 蛋白, Mouse (AAA02562.1, HEK293, His)

Siglec-2/CD22 Protein mediates B-cell B-cell interaction and may be involved in the localization of B-cells in lymphoid tissues^[1]. Siglec-2/CD22 Protein, Mouse (AAA02562.1, HEK293, His) is the recombinant mouse-derived Siglec-2/CD22 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Siglec-2/CD22 Protein, Mouse (AAA02562.1, HEK293, His) is 681 a.a., with molecular weight of 100-120 kDa.

Siglec-2/CD22 蛋白是一种 B 细胞限制性糖蛋白，在 B 祖细胞和前 B 细胞的细胞质以及成熟 B 细胞的表面表达^[2]。Siglec-2/CD22 蛋白是一种粘附分子，优先结合相同（顺式）或相邻（反式）细胞上的 α 2,6 连接唾液酸^[3]。Siglec-2/CD22 蛋白是一种辅助受体，与 B 细胞受体 (BCR) 发生物理相互作用，并在 BCR 连接后迅速磷酸化。它通过将酪氨酸磷酸酶 SHP-1 募集至其 ITIM 来负向调节 BCR 信号^[4]。

Mouse

HEK293

C-6*His

AAA02562.1 (S22-R702)

12483  [NCBI]

SANDWTVDHPQTLFAWEGACIRIPCKYKTPLPKARLDNILLFQNYEFDKATKKFKGTVLYNKAEPELYPPKQRRVTFLGNSIDNCTLKIHPIRANDSGNLGLRMTAGTERWMEPIHLNVSEKPFQPYIQMPSEIRESQSVTLTCGLNFSCFEYDILLQWFLEDSKITSVTPSVTSITSSVTSSIKNVYTESKLTFQPKWTDHGKSVKCQVQHSSEVLSERTVRLDVKYTPKLEIKVNPTEVEKNNSVTMTCRVNSSNPKLRTVAVSWFKDGRPLEDQELEQEQQMSKLILHSVTKDMRGKYRCQASNDIGPGESEEVELTVHYAPEPSRVHIYPSPAEEGQSVELICESLASPSATNYTWYHNRKPIPGDTQEKLRIPKVSPWHAGNYSCLAENRLGHGKIDQEAKLDVHYAPKAVTTVIQSFTPILEGDSVTLVCRYNSSNPDVTSYRWNPQGSGSVLKPGVLRIQKVTWDSMPVSCAACNHKCSWALPVILNVHYAPRDVKVLKVSPASEIRAGQRVLLQCDFAESNPAEVRFFWKKNGSLVQEGRYLSFGSVSPEDSGNYNCMVNNSIGETLSQAWNLQVLYAPRRLRVSISPGDHVMEGKKATLSCESDANPPISQYTWFDSSGQDLHSSGQKLRLEPLEVQHTGSYRCKGTNGIGTGESPPSTLTVYYSPETIGKR

100-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.