TGF alpha/TGFA 蛋白, Human

TGF-alpha Protein, Human is a 50-amino-acid polypeptide that binds to the epidermal growth factor (EGF) receptor and stimulates cell growth.

转化生长因子α（TGF-α）是乳腺正常和肿瘤发育的主要分子。TGF-α 与表皮生长因子受体 (EGFR) 结合，激活 EGFR 的内源性酪氨酸激酶活性，并刺激处女和怀孕小鼠乳腺上皮的生长。体内外乳腺癌细胞均可检测到 TGF-α 表达，过度表达可引起人类和啮齿动物乳腺上皮细胞部分转化或永生化^[1][2] 。

The ED₅₀ is <0.2 ng/mL as measured by BALB/c 3T3 cells, corresponding to a specific activity of >5 × 10⁶ units/mg.

Human

E. coli

Tag Free

P01135 (V40-A89)

7039  [NCBI]

VVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA

Approximately 5.8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Nilsson O, et al. Expression of transforming growth factor alpha and its receptor in human neuroendocrine tumours. Int J Cancer. 1995 Mar 3;60(5):645-51.  [Content Brief]

  [2]. Lupia E, et al. Thrombopoietin as biomarker and mediator of cardiovascular damage in critical diseases. Mediators Inflamm. 2012;2012:390892.  [Content Brief]