TGFBR1/ALK-5 蛋白, Mouse (HEK293, Fc)

TGFBR1 is a transmembrane kinase that cooperates with TGFBR2 to form the TGF-β receptor complex.This complex is activated by cytokines and regulates a variety of processes, including cell cycle arrest, mesenchymal cell control, wound healing, and carcinogenesis.TGFBR1/ALK-5 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived TGFBR1/ALK-5 protein, expressed by HEK293 , with C-hFc labeled tag.

TGFBR1是一种跨膜丝氨酸/苏氨酸激酶，与TGF-β II型丝氨酸/苏氨酸激酶受体TGFBR2形成复合物，构成TGF-β细胞因子的特异性受体，包括TGFB1、TGFB2和TGFB3。这种受体复合物在将信号从细胞表面转导到细胞质中发挥着至关重要的作用，从而调节多种生理和病理过程。这些包括诱导上皮细胞和造血细胞的细胞周期停滞、控制间充质细胞增殖和分化、影响伤口愈合、细胞外基质产生、免疫抑制和参与癌变。受体复合物由两个 TGFBR1 和两个 TGFBR2 分子对称地结合到细胞因子二聚体组成，其组装导致具有催化活性的 TGFBR2 对 TGFBR1 的组成型激活。激活的 TGFBR1 磷酸化 SMAD2，导致其与受体解离并随后与 SMAD4 相互作用。由此产生的 SMAD2-SMAD4 复合物易位到细胞核，在细胞核中调节 TGF-β 调节基因的转录，启动典型的 SMAD 依赖性 TGF-β 信号级联反应。此外，TGFBR1 还参与非典型的、不依赖 SMAD 的 TGF-β 信号通路。例如，它诱导 TRAF6 自动泛素化，导致 MAP3K7 泛素化和激活，引发细胞凋亡。此外，TGFBR1 通过涉及 PARD6A 磷酸化和激活的 SMAD 独立信号通路来调节上皮细胞向间质细胞的转变。

Measured  by its binding ability in a functional ELISA. When Recombinant Human TGF-beta  RII is immobilized at 1 μg/mL (100 μL/well), it binds Recombinant  Human TGF-beta RI in the presence of TGF-beta 1. The concentration of  rhTGF-beta RI that produces 50% of the optimal binding response is  approximately 0.835 μg/mL.

Mouse

HEK293

C-hFc

Q64729 (L30-E125)

21812  [NCBI]

LQCFCHLCTKDNFTCETDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGAVTTTYCCNQDHCNKIELPTTGPFSEKQSAGLGPVE

40-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.