CRF, bovine (Synonyms: Corticotropin Releasing Factor bovine)

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CRF, bovine 是一种有效的 CRF 受体激动剂，能够取代 [¹²⁵I-Tyr]ovine CRF，K_i 值为3.52 nM。

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CRF, bovine Chemical Structure

CAS No. : 92307-52-3

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CRF, bovine 的其他形式现货产品:

CRF, bovine TFA

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Other Forms of CRF, bovine:

CRF, bovine TFA In-stock

MCE 顾客使用本产品发表的 1 篇科研文献

•Sci Adv. 2024 May 10;10(19):eadk7636. [Abstract]

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参考文献

生物活性	CRF, bovine is a potent agonist of CRF receptor, and displaces [¹²⁵I-Tyr]ovine CRF with a K_i of 3.52 nM.
IC₅₀ & Target	Ki: 3.52 nM (CRF receptor)^[1]
体外研究 (In Vitro)	CRF, bovine is a potent agonist of CRF receptor, and displaces [¹²⁵I-Tyr]ovine CRF with a K_i of 3.52 nM^[1]. CRF shows pEC₅₀s of 11.16, 8.53 and 8.70 for human CRF₁, human CRF₂ and rat CRF_2α^[2]. CRF is released from hypothalamic-pituitary-adrenal (HPA) axis induced by stress, and leads to production of glucocorticoids which down regulate immune responses. CRF also has proinflammatory effects. CRF affects brain microvessel endothelial cells (BMEC) structure or function, CRF (100 nM) significantly increases cAMP in BMEC^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. CRF, bovine 相关抗体:
分子量	4697.34
Formula	C₂₀₆H₃₄₀N₆₀O₆₃S
CAS 号	92307-52-3
Sequence	Ser-Gln-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Thr-Lys-Ala-Asp-Gln-Leu-Ala-Gln-Gln-Ala-His-Asn-Asn-Arg-Lys-Leu-Leu-Asp-Ile-Ala-NH2
Sequence Shortening	SQEPPISLDLTFHLLREVLEMTKADQLAQQAHNNRKLLDIA-NH2
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料	Data Sheet (522 KB) 产品使用指南 (1538 KB)
参考文献	[1]. Hogg JE, et al. The human neuroblastoma cell line, IMR-32, expresses functional corticotropin-releasing factor receptors. Eur J Pharmacol. 1996 Sep 26;312(2):257-61. [Content Brief] [2]. Smart D, et al. Characterisation using microphysiometry of CRF receptor pharmacology. Eur J Pharmacol. 1999 Aug 27;379(2-3):229-35. [Content Brief] [3]. Esposito P, et al. Corticotropin-releasing factor (CRF) can directly affect brain microvessel endothelial cells. Brain Res. 2003 Apr 11;968(2):192-8. [Content Brief]

Cell Assay ^[3]	CRF (1 μM) is added to the cell cultures that are further incubated for 5, 15 and 30 min at 37°C. Control cells are incubated with medium only. Following incubation time, cells are lysed directly on the growth dish using the detergent provided by the cAMP enzyme immunoassay kit. Following trypan blue staining to ensure complete lysis, the cell lysate is collected and assayed for cAMP. In some cases, the brain microvessel endothelial cells (BMEC) are treated with forskolin or pretreated either with the CRFR antagonist Antalarmin (1 μM) or the ATP analogue 2′5′-deoxyadenosine for 5 min at 37°C^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Hogg JE, et al. The human neuroblastoma cell line, IMR-32, expresses functional corticotropin-releasing factor receptors. Eur J Pharmacol. 1996 Sep 26;312(2):257-61. [Content Brief] [2]. Smart D, et al. Characterisation using microphysiometry of CRF receptor pharmacology. Eur J Pharmacol. 1999 Aug 27;379(2-3):229-35. [Content Brief] [3]. Esposito P, et al. Corticotropin-releasing factor (CRF) can directly affect brain microvessel endothelial cells. Brain Res. 2003 Apr 11;968(2):192-8. [Content Brief]

Cell Assay
^[3]

CRF (1 μM) is added to the cell cultures that are further incubated for 5, 15 and 30 min at 37°C. Control cells are incubated with medium only. Following incubation time, cells are lysed directly on the growth dish using the detergent provided by the cAMP enzyme immunoassay kit. Following trypan blue staining to ensure complete lysis, the cell lysate is collected and assayed for cAMP. In some cases, the brain microvessel endothelial cells (BMEC) are treated with forskolin or pretreated either with the CRFR antagonist Antalarmin (1 μM) or the ATP analogue 2′5′-deoxyadenosine for 5 min at 37°C^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Hogg JE, et al. The human neuroblastoma cell line, IMR-32, expresses functional corticotropin-releasing factor receptors. Eur J Pharmacol. 1996 Sep 26;312(2):257-61. [Content Brief]

[2]. Smart D, et al. Characterisation using microphysiometry of CRF receptor pharmacology. Eur J Pharmacol. 1999 Aug 27;379(2-3):229-35. [Content Brief]

[3]. Esposito P, et al. Corticotropin-releasing factor (CRF) can directly affect brain microvessel endothelial cells. Brain Res. 2003 Apr 11;968(2):192-8. [Content Brief]

CRF, bovine 相关分类

Inflammation/Immunology Endocrinology

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CRF, bovine92307-52-3Corticotropin Releasing Factor bovineCRFRCorticotropin-releasing Factor ReceptorCRHRInhibitorinhibitorinhibit

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CRF, bovine (Synonyms: Corticotropin Releasing Factor bovine)

Customer Review

Other Forms of CRF, bovine:

MCE 顾客使用本产品发表的 1 篇科研文献

CRF, bovine 相关产品

•同靶点产品:

•同靶点蛋白产品:

查看 CRFR 亚型特异性产品:

生物活性

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纯度 & 产品资料

参考文献

CRF, bovine 相关分类

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CRF, bovine (Synonyms: Corticotropin Releasing Factor bovine)

Customer Review

Other Forms of CRF, bovine:

CRF, bovine 相关抗体

MCE 顾客使用本产品发表的 1 篇科研文献

CRF, bovine 相关产品

•同靶点产品:

•同靶点蛋白产品:

查看 CRFR 亚型特异性产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

CRF, bovine 相关抗体:

CRF, bovine 相关分类

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The molarity calculator equation

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