Corynoxeine (Synonyms: 去氢钩藤碱)

目录号: HY-N0590 纯度: 99.97%: FAQs
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Corynoxeine, 从 Uncaria rhynchophylla 的钩中分离的，是 PDGF-BB 诱导血管平滑肌细胞 (VSMCs) 增殖过程中的一种有效 ERK1/ERK 2 抑制剂。

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Corynoxeine Chemical Structure

CAS No. : 630-94-4

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规格	价格	是否有货
1 mg	￥361	In-stock
5 mg	￥692	In-stock
10 mg	￥900	In-stock
25 mg	￥1538	In-stock
50 mg	￥2288	In-stock
100 mg	￥3438	In-stock
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500 mg		询价

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Other Forms of Corynoxeine:

Corynoxeine (Standard) 询价

MCE 顾客使用本产品发表的 5 篇科研文献

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ERK ERK1 ERK2 ERK5 ERK8

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Corynoxeine, isolated from the hook of Uncaria rhynchophylla, is a potent ERK1/ERK2 inhibitor of key PDGF-BB-induced vascular smooth muscle cells (VSMCs) proliferation.

IC₅₀ & Target^[1]

ERK1

ERK2

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
Microglia	IC₅₀	15.7 μM Compound: 3	Inhibition of LPS-stimulated NO release in rat cortical microglial cells assessed as nitrite accumulation after 48 hrs Inhibition of LPS-stimulated NO release in rat cortical microglial cells assessed as nitrite accumulation after 48 hrs	[PMID: 18588343]

体外研究
(In Vitro)

Corynoxeine 能通过下调 PDGF-BB 诱导的 ERK1/2 激活抑制 PDGF-BB 刺激的血管平滑肌细胞 (VSMCs) 增殖。将 VSMCs 与 Corynoxeine 预孵育，可显著抑制 PDGF-BB 诱导的细胞外信号调节激酶 1/2 (ERK1/2) 激活，而对丝裂原活化蛋白激酶 (MAPK/ERK) 激活激酶 1 和 2 (MEK1/2)、Akt、磷脂酶 C (PLC) γ1 的激活或 PDGF 受体 β (PDGF-Rβ) 的磷酸化无影响。Corynoxeine 抑制 PDGF-BB 诱导的 ERK1/2 激活，其作用浓度范围与抑制 VSMC 增殖和 DNA 合成的浓度相同。Corynoxeine 对 PDGF-BB 诱导的 VSMC 数量的抑制作用的半数抑制浓度 (IC₅₀) 为 13.7 μM，对 PDGF-BB 诱导的 DNA 合成 (24 小时) 的 IC₅₀ 为 9.2 μM。预处理 VSMCs (5-50 μM) 24 小时可显著减少细胞数量且无细胞毒性；抑制率分别为 25.0±12.5%、63.0±27.5% 和 88.0±12.5% (5、20 和 50 μM)。Corynoxeine 还可以浓度依赖性方式显著抑制 50 ng/mL PDGF-BB 诱导的 VSMCs DNA 合成且无细胞毒性；在 5、20 和 50 μM 浓度下的抑制率分别为 32.8±11.0%、51.8±8.0% 和 76.9±7.4%^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

382.45

Formula

C₂₂H₂₆N₂O₄

CAS 号

630-94-4

性状

固体

颜色

White to off-white

中文名称

去氢钩藤碱；柯诺辛因碱

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 3.8 mg/mL (9.94 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.6147 mL	13.0736 mL	26.1472 mL
5 mM	0.5229 mL	2.6147 mL	5.2294 mL
10 mM	---	---	---

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

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每只动物的给药体积

μL

动物数量

只

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计算结果

工作液所需浓度 : mg/mL

纯度 & 产品资料

纯度: 99.97%

选择批次：

Data Sheet (623 KB) SDS (251 KB)

COA (289 KB) HNMR (281 KB) LCMS (93 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Kim TJ, et al. Corynoxeine isolated from the hook of Uncaria rhynchophylla inhibits rat aortic vascular smooth muscle cell proliferation through the blocking of extracellular signal regulated kinase 1/2 phosphorylation. Biol Pharm Bull. 2008 Nov;31(11):2073-8. [Content Brief]

Cell Assay ^[1]	Cell proliferation and DNA synthesis are measured. For cell counting, VSMCs are seeded in 12-well culture plates at 5-6×10⁴ cells/mL and cultured in DMEM with 10% FBS at 37°C for 24 h. Under these conditions, the cells reach 70% confluence. The medium is then replaced by serum-free medium with Corynoxeine (5-50 μM). The cells are stimulated with 50 ng/mL PDGF-BB, then trypsinized with trypsin-EDTA and counted using a hemocytometer under a microscope. For [³H]-thymidine incorporation experiments, VSMCs are seeded in 24-well culture plates 5000 cells/well and then allowed to grow for 3-4 d in DMEM, and 2 μCi/mL of [³H]-thymidine are added to the medium. The reactions are terminated after 4 h by aspirating the medium and subjecting the cultures to sequential washes on ice with PBS containing 10% trichloroacetic acid and ethanol/ether (1 : 1, v/v). Acid-insoluble [³H]-thymidine is extracted into 250 μL of 0.5 M NaOH/well; this solution is then mixed with 3ml of scintillation cocktail and quantified using a liquid scintillation counter^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Kim TJ, et al. Corynoxeine isolated from the hook of Uncaria rhynchophylla inhibits rat aortic vascular smooth muscle cell proliferation through the blocking of extracellular signal regulated kinase 1/2 phosphorylation. Biol Pharm Bull. 2008 Nov;31(11):2073-8. [Content Brief]

Cell Assay
^[1]

Cell proliferation and DNA synthesis are measured. For cell counting, VSMCs are seeded in 12-well culture plates at 5-6×10⁴ cells/mL and cultured in DMEM with 10% FBS at 37°C for 24 h. Under these conditions, the cells reach 70% confluence. The medium is then replaced by serum-free medium with Corynoxeine (5-50 μM). The cells are stimulated with 50 ng/mL PDGF-BB, then trypsinized with trypsin-EDTA and counted using a hemocytometer under a microscope. For [³H]-thymidine incorporation experiments, VSMCs are seeded in 24-well culture plates 5000 cells/well and then allowed to grow for 3-4 d in DMEM, and 2 μCi/mL of [³H]-thymidine are added to the medium. The reactions are terminated after 4 h by aspirating the medium and subjecting the cultures to sequential washes on ice with PBS containing 10% trichloroacetic acid and ethanol/ether (1 : 1, v/v). Acid-insoluble [³H]-thymidine is extracted into 250 μL of 0.5 M NaOH/well; this solution is then mixed with 3ml of scintillation cocktail and quantified using a liquid scintillation counter^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Kim TJ, et al. Corynoxeine isolated from the hook of Uncaria rhynchophylla inhibits rat aortic vascular smooth muscle cell proliferation through the blocking of extracellular signal regulated kinase 1/2 phosphorylation. Biol Pharm Bull. 2008 Nov;31(11):2073-8. [Content Brief]

Corynoxeine 相关分类

Cardiovascular Disease

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6147 mL	13.0736 mL	26.1472 mL	65.3680 mL
DMSO	5 mM	0.5229 mL	2.6147 mL	5.2294 mL	13.0736 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Corynoxeine630-94-4去氢钩藤碱柯诺辛因碱ERKExtracellular signal regulated kinasesInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Corynoxeine (Synonyms: 去氢钩藤碱)

Customer Review

Other Forms of Corynoxeine:

MCE 顾客使用本产品发表的 5 篇科研文献