G-418 disulfate (Synonyms: Geneticin sulfate; Antibiotic G-418 sulfate)

目录号: HY-17561 纯度: 99.75%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

G-418 disulfate (Geneticin sulfate) 是一种氨基糖苷类抗生素，抑制真核生物和原核生物的蛋白质合成。G-418 disulfate 是一种常用的真核细胞选择性药剂。

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G-418 disulfate Chemical Structure

CAS No. : 108321-42-2

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规格	价格	是否有货
10 mM * 1 mL in Water	￥500	In-stock
500 mg	￥400	In-stock
1 g	￥550	In-stock
5 g	￥2200	In-stock
10 g		询价
50 g		询价

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Other Forms of G-418 disulfate:

G-418 询价

MCE 顾客使用本产品发表的 33 篇科研文献

G-418 disulfate 相关产品

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Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

G-418 disulfate (Geneticin sulfate), is an aminoglycoside antibiotic, inhibits protein synthesis in eukaryotes and prokaryotes. G-418 disulfate is commonly used as a selective agent for eukaryotic cells^[1].

IC₅₀ & Target

Aminoglycoside

体外研究
(In Vitro)

G418 suLfate 是一种氨基糖苷类新霉素类似物，可干扰蛋白质合成，已广泛用于选择具有新霉素抗性 (NR) 的哺乳动物细胞系。新霉素抗性 (neo) 基因在真核载体中经常用作显性可选择基因。G418 可与脱唾液酸粘蛋白 (AsOR) 共价结合，形成针对肝细胞特异性靶向和毒性的偶联物^[2]。
将人GD3合酶cDNA转染MDA-MB231细胞，采用G-418批量筛选法筛选出稳定过表达GD3合酶的细胞^[2]。氨基糖苷类抗生素 G418 已被证明是许多原核生物和真核生物的抑制剂，浓度为 1-300 μg/mL^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

692.71

Formula

C₂₀H₄₄N₄O₁₈S₂

CAS 号

108321-42-2

性状

固体

颜色

White to off-white

中文名称

遗传霉素硫酸盐

结构分类

初始来源

微生物

Micromonospora rhodorangea

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

溶解性数据

细胞实验：

H₂O 中的溶解度 : 125 mg/mL (180.45 mM; 超声助溶)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.4436 mL	7.2180 mL	14.4361 mL
5 mM	0.2887 mL	1.4436 mL	2.8872 mL
10 mM	0.1444 mL	0.7218 mL	1.4436 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 50 mg/mL (72.18 mM); 澄清溶液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.75%

选择批次：

Data Sheet (644 KB) SDS (393 KB)

COA (304 KB) HNMR (288 KB) RP-HPLC (278 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Giordano-Santini R, et al. An antibiotic selection marker for nematode transgenesis. Nat Methods. 2010;7(9):721-723. [Content Brief]

[2]. Volarevic M, et al. A novel G418 conjugate results in targeted selection of genetically protected hepatocytes without bystander toxicity. Bioconjug Chem. 2007;18(6):1965-1971. [Content Brief]

[3]. Kwon KM, et al. Disialyl GD2 ganglioside suppresses ICAM-1-mediated invasiveness in human breast cancer MDA-MB231 cells. Int J Biol Sci. 2017;13(3):265-275. Published 2017 Feb 12. [Content Brief]

[4]. Davies J, et al. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg. 1980;29(5 Suppl):1089-1092. [Content Brief]

Animal Administration ^[3]	To characterize the sensitivity of the trypanosome populations to G418 in vivo, bloodstream forms of T. brucei brucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3 are expanded separately in sublethally irradiated mice. Prior to the first peak of parasitemia, trypanosomes are collected, and aliquots containing 106 trypanosomes are inoculated intraperitoneally into mice. Twenty-four hours following infection, the mice are divided into groups and treated with G418 at a dose of 10, 20, 30, 40, 50, or 80 mg/kg of body weight (bw) by inoculating intraperitoneally 0.2 mL of the drug in sterile water. At 24 and 48 h following the first treatment, G418 is administered to animals in each group at the same dose as before, resulting in three treatments per mouse. Repeated drug treatments are necessary to ensure complete elimination of nontransfected GUTat 3.1 parasites from the mice. Mice are then monitored daily, for 33 days, for the presence of parasites by microscopic examination of wet-blood films. Animals found to be parasitemic are recorded and then removed from the experiment. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Giordano-Santini R, et al. An antibiotic selection marker for nematode transgenesis. Nat Methods. 2010;7(9):721-723. [Content Brief] [2]. Volarevic M, et al. A novel G418 conjugate results in targeted selection of genetically protected hepatocytes without bystander toxicity. Bioconjug Chem. 2007;18(6):1965-1971. [Content Brief] [3]. Kwon KM, et al. Disialyl GD2 ganglioside suppresses ICAM-1-mediated invasiveness in human breast cancer MDA-MB231 cells. Int J Biol Sci. 2017;13(3):265-275. Published 2017 Feb 12. [Content Brief] [4]. Davies J, et al. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg. 1980;29(5 Suppl):1089-1092. [Content Brief]

Animal Administration
^[3]

To characterize the sensitivity of the trypanosome populations to G418 in vivo, bloodstream forms of T. brucei brucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3 are expanded separately in sublethally irradiated mice. Prior to the first peak of parasitemia, trypanosomes are collected, and aliquots containing 106 trypanosomes are inoculated intraperitoneally into mice. Twenty-four hours following infection, the mice are divided into groups and treated with G418 at a dose of 10, 20, 30, 40, 50, or 80 mg/kg of body weight (bw) by inoculating intraperitoneally 0.2 mL of the drug in sterile water. At 24 and 48 h following the first treatment, G418 is administered to animals in each group at the same dose as before, resulting in three treatments per mouse. Repeated drug treatments are necessary to ensure complete elimination of nontransfected GUTat 3.1 parasites from the mice. Mice are then monitored daily, for 33 days, for the presence of parasites by microscopic examination of wet-blood films. Animals found to be parasitemic are recorded and then removed from the experiment.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Giordano-Santini R, et al. An antibiotic selection marker for nematode transgenesis. Nat Methods. 2010;7(9):721-723. [Content Brief]

[2]. Volarevic M, et al. A novel G418 conjugate results in targeted selection of genetically protected hepatocytes without bystander toxicity. Bioconjug Chem. 2007;18(6):1965-1971. [Content Brief]

[3]. Kwon KM, et al. Disialyl GD2 ganglioside suppresses ICAM-1-mediated invasiveness in human breast cancer MDA-MB231 cells. Int J Biol Sci. 2017;13(3):265-275. Published 2017 Feb 12. [Content Brief]

[4]. Davies J, et al. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg. 1980;29(5 Suppl):1089-1092. [Content Brief]

G-418 disulfate 相关分类

Infection

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.4436 mL	7.2180 mL	14.4361 mL	36.0901 mL
	5 mM	0.2887 mL	1.4436 mL	2.8872 mL	7.2180 mL
	10 mM	0.1444 mL	0.7218 mL	1.4436 mL	3.6090 mL
	15 mM	0.0962 mL	0.4812 mL	0.9624 mL	2.4060 mL
	20 mM	0.0722 mL	0.3609 mL	0.7218 mL	1.8045 mL
	25 mM	0.0577 mL	0.2887 mL	0.5774 mL	1.4436 mL
	30 mM	0.0481 mL	0.2406 mL	0.4812 mL	1.2030 mL
	40 mM	0.0361 mL	0.1805 mL	0.3609 mL	0.9023 mL
	50 mM	0.0289 mL	0.1444 mL	0.2887 mL	0.7218 mL
	60 mM	0.0241 mL	0.1203 mL	0.2406 mL	0.6015 mL
	80 mM	0.0180 mL	0.0902 mL	0.1805 mL	0.4511 mL
	100 mM	0.0144 mL	0.0722 mL	0.1444 mL	0.3609 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

G-418 disulfate108321-42-2GeneticinAntibiotic G-418遗传霉素硫酸盐Antibiotic G418Antibiotic G 418BacterialAntibioticproteinsynthesiseukaryoticcellsInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

G-418 disulfate (Synonyms: Geneticin sulfate; Antibiotic G-418 sulfate)

Customer Review

Other Forms of G-418 disulfate:

MCE 顾客使用本产品发表的 33 篇科研文献