2. Immunology/Inflammation
  3. NO Synthase
  4. L-Canavanine sulfate

L-Canavanine sulfate 

目录号: HY-B1581A 纯度: 99.39%
COA 产品使用指南

摩尔计算器

L-Canavanine sulfate 是诱导型 NO 合酶 (NO synthase) 的选择性抑制剂。

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L-Canavanine sulfate Chemical Structure

L-Canavanine sulfate Chemical Structure

CAS No. : 2219-31-0

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥440
In-stock
50 mg ￥400
In-stock
100 mg ￥600
In-stock
200 mg   询价  
500 mg   询价  

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Customer Review

Other Forms of L-Canavanine sulfate:

L-Canavanine sulfate 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

IF

    L-Canavanine sulfate purchased from MCE. Usage Cited in: Oncol Rep. 2018 Jun;39(6):2595-2603.  [Abstract]

    The effect of CuD (2 μM), SC79 (4 μg/mL), and L‑canavanine (1 mM) on cell apoptosis in the AGS cell line.

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    nNOS iNOS eNOS

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    L-Canavanine sulfate is a selective inhibitor of inducible NO synthase.

    IC50 & Target

    NO synthase[1]
    体外研究
    (In Vitro)

    L-Canavanine sulfate (L-CAV) causes only a limited degree of cytotoxicity in HeLa, Hep G2, and SK-HEP-1 cells when given alone in arginine-rich media with IC50 values ranging from 5 to 10 mM. In HaCaT keratinocyte cell line, IC50 of L-Canavanine sulfate exceeds the concentration of 10 mM, indicating low cytotoxicity in normal cells in vitro. In arginine-free media, IC50 of L-Canavanine sulfate in HeLa, Hep G2, and SK-HEP-1 cells are 0.21±0.04; 0.64±0.16; and 1.18±0.14 mM, respectively. L-Canavanine sulfate, which is hardly toxic alone, potentiates the cytotoxicity of vinblastine (VIN) and paclitaxel (PTX) in HeLa and hepatocellular carcinoma cells[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Canavanine sulfate 相关抗体:
    体内研究
    (In Vivo)

    Administration of L-Canavanine sulfate (100 mg/kg) produces a moderate increase in mean arterial pressure of 20 mm Hg, returns blood pressure to near basal levels and completely attenuates the lipopolysaccharide-induced hypotension. All, but one, endotoxaemic rats dosed with L-Canavanine sulfate (100 mg/kg) survive for 6 h post lipopolysaccharide, after which time the experiment is terminated (n=7)[1]. L-canavanine inhibits DNA synthesis by Li 210 cells in vivo and significantly increases the lifespan of animals bearing the Li 210 leukemia. An optimal dose of 18 g/kg produces a peak increase in lifespan of 44%. The therapeutic dose range is narrow, and a dose of 24 g/kg causes death due to drug toxicity[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    274.25

    Formula

    C5H14N4O7S
    CAS 号
    性状

    固体

    颜色

    White to off-white

    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 100 mg/mL (364.63 mM; 超声加热助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.6463 mL 18.2315 mL 36.4631 mL
    5 mM 0.7293 mL 3.6463 mL 7.2926 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 50 mg/mL (182.32 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.39%

    COA (286 KB) HNMR (248 KB) RP-HPLC (279 KB) MS (68 KB) OR (639 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [2]

    A dose-dependent cytotoxicity is examined using the MTT assay. Into each well of 96-well plates, 2×104 of cells are seeded, and after 24 h incubation, cells are incubated with test compounds (including L-Canavanine sulfate). After 24 h, 0.5 mg/mL of MTT is added to each well of HeLa, Caco-2, MIA PaCa-2, BxPC-3 and SK-HEP-1 cells, while MTT is added to Hep G2 after 48 h incubation with test compounds (including L-Canavanine sulfate). The cells are then incubated for 3 h so that the viable cells could produce formazan crystals; they are then dissolved in 100 μL DMSO. After incubation for 10 min in a shaker, the absorption of the formazan is measured at 570 nm using a reader[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Male rats weighing 295 to 305 g are used. After a period of stabilization, 6 mg lipopolysaccharide/kg is administered intravenously in 0.3 mL over 2 min, and cardiovascular parameters are monitored over 5 to 6 h. L-Canavanine sulfate is administered intravenously in a 0.2 mL volume bolus injection at 100 mg/kg[1].

    Mice: L-Canavanine is dissolved in phosphate-buffered saline[1]. L-Canavanine (100 mg/mb) is infused at a rate of 0.1 mL/hr through a catheter implanted S.C. over the back. Groups of 5 mice are treated at each dose beveltogether with 6 to 8 control animals and are inspected twice per day for median time of death[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    L-Canavanine sulfate 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 3.6463 mL 18.2315 mL 36.4631 mL 91.1577 mL
    5 mM 0.7293 mL 3.6463 mL 7.2926 mL 18.2315 mL
    10 mM 0.3646 mL 1.8232 mL 3.6463 mL 9.1158 mL
    15 mM 0.2431 mL 1.2154 mL 2.4309 mL 6.0772 mL
    20 mM 0.1823 mL 0.9116 mL 1.8232 mL 4.5579 mL
    25 mM 0.1459 mL 0.7293 mL 1.4585 mL 3.6463 mL
    30 mM 0.1215 mL 0.6077 mL 1.2154 mL 3.0386 mL
    40 mM 0.0912 mL 0.4558 mL 0.9116 mL 2.2789 mL
    50 mM 0.0729 mL 0.3646 mL 0.7293 mL 1.8232 mL
    60 mM 0.0608 mL 0.3039 mL 0.6077 mL 1.5193 mL
    80 mM 0.0456 mL 0.2279 mL 0.4558 mL 1.1395 mL
    100 mM 0.0365 mL 0.1823 mL 0.3646 mL 0.9116 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Canavanine2219-31-0NO SynthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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