2. Membrane Transporter/Ion Channel Neuronal Signaling Immunology/Inflammation
  3. iGluR NO Synthase
  4. Tat-NR2B9c

Tat-NR2B9c  (Synonyms: Tat-NR2Bct; NA-1)

目录号: HY-P0117 纯度: 99.97%
COA 产品使用指南

摩尔计算器

Tat-NR2B9c (Tat-NR2Bct; NA-1) 是一种 PSD-95 的抑制剂，抑制 PSD-95d2 (PSD-95 PDZ domain 2) 和 PSD-95d1 的 EC50 分别为 6.7 nM 和 670 nM。Tat-NR2B9c 扰乱了 PSD-95/NMDAR 的相互作用，抑制 PSD-95 和 NR2A 和 NR2B 结合的 IC50 值分别为 0.5 μM 和 8 μM。Tat-NR2B9c 还能抑制神经元一氧化氮合酶 (nNOS)/PSD-95 相互作用，具有神经保护作用。

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Tat-NR2B9c Chemical Structure

Tat-NR2B9c Chemical Structure

CAS No. : 500992-11-0

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3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥6096
In-stock
1 mg ￥700
In-stock
5 mg ￥1800
In-stock
10 mg ￥2800
In-stock
25 mg ￥5500
In-stock
50 mg   询价  
100 mg   询价  

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Customer Review

Other Forms of Tat-NR2B9c:

Tat-NR2B9c 相关抗体

Top Publications Citing Use of Products

    Tat-NR2B9c purchased from MCE. Usage Cited in: J Cereb Blood Flow Metab. 2019 Aug;39(8):1588-1601.  [Abstract]

    Tat-NR2B9c affects PSD95-NR2B-nNOS complex and PSD95-neurexin-neuroligin complex formation after ICH. Western blots of IP with PSD95 antibody show the interactions between nNOS, NR2B, neurexin-1 or neuroligin-1, and PSD95.

    查看 iGluR 亚型特异性产品:

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    AMPA Receptor NMDA Receptor Kainate Receptor

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    nNOS iNOS eNOS

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Tat-NR2B9c (Tat-NR2Bct; NA-1) is a postsynaptic density-95 (PSD-95) inhibitor, with EC50 values of 6.7 nM and 670 nM for PSD-95d2 (PSD-95 PDZ domain 2) and PSD-95d1, respectively. Tat-NR2B9c disrupts the PSD-95/NMDAR interaction, inhibiting NR2A and NR2B binding to PSD-95 with IC50 values of 0.5 μM and 8 μM, respectively. Tat-NR2B9c also inhibits neuronal nitric oxide synthase (nNOS)/PSD-95 interaction, and possesses neuroprotective efficacy[1][2][5].

    IC50 & Target

    NMDA Receptor

     

    nNOS

     

    体外研究
    (In Vitro)

    Tat-NR2B9c is a PSD-95 inhibitor, with an EC50 of 6.7 nM for PSD-95d2, representing a >100-fold higher affinity for this domain than for PSD-95d1 (EC50, 0.67 μM). Tat-NR2B9c inhibits NMDAR2A, NMDAR2B, and NMDAR2C binding to PSD-95, with IC50s of 0.5 μM, ∼8 μM, and 0.75 μM, respectively.
    Tat-NR2B9c also blocks the interaction between PSD-95 and nNOS with an IC50 of ∼0.2 μM[1].
    Tat-NR2B9c reduces association of PSD-95 with GluN2B by ∼50% in the YAC128 striatum, decreases NMDA-induced p38 activation in YAC128 striatal tissue, but shows no effect on the NMDA-induced JNK activation[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Tat-NR2B9c 相关抗体:
    体内研究
    (In Vivo)

    Tat-NR2B9c (10 nmol/g, i.v.) reduces infarction volume of male C57BL/6 mice, but has no effect at 3 nM/g[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    2518.88

    Formula

    C105H188N42O30
    CAS 号
    性状

    固体

    颜色

    White to off-white

    Sequence Shortening

    YGRKKRRQRRRKLSSIESDV
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 100 mg/mL (39.70 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 0.3970 mL 1.9850 mL 3.9700 mL
    5 mM 0.0794 mL 0.3970 mL 0.7940 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案源自文献，仅供参考，建议您先取少量样品进行尝试。

    • 方案 一

      Tat-NR2B9c is prepared in saline[4].

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.97%

    COA (284 KB) RP-HPLC (109 KB) MS (218 KB) 元素分析报告 (208 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [2]

    Postnatal mono-cultured WT and YAC128 striatal neurons (DIV 9, due to the viability of these mono-cultured MSNs) are pretreated for 1 h with 200 nM Tat-NR2B9c, and/or SB-239063 (p38 inhibitor), and/or SP-600125 (JNK inhibitor), then incubated with or without 500 μM NMDA for 10 min. After NMDA treatment, striatal neurons are washed once with warm plating medium (PM) and then incubated in conditioned PM (without Tat peptides or p38, JNK inhibitors) for 24 h. Then cells are washed with PBS once and fixed with 4% paraformaldehyde (PFA) for 30 min[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    Mice[3]
    In each study mice are randomly allocated to three treatment groups (0.0, 3.0, 10.0 nMole/g Tat-NR2B9c) or to sham treatment. The individual performing the experimental procedures, administering treatments and performing the analyses is blinded to the treatment assignments. Tat-NR2B9c is prepared at the indicated doses and administered intravenously via the tail vein using a pump in a volume of 1 µL/g over 5 min beginning at reperfusion[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Tat-NR2B9c 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 0.3970 mL 1.9850 mL 3.9700 mL 9.9250 mL
    5 mM 0.0794 mL 0.3970 mL 0.7940 mL 1.9850 mL
    10 mM 0.0397 mL 0.1985 mL 0.3970 mL 0.9925 mL
    15 mM 0.0265 mL 0.1323 mL 0.2647 mL 0.6617 mL
    20 mM 0.0199 mL 0.0993 mL 0.1985 mL 0.4963 mL
    25 mM 0.0159 mL 0.0794 mL 0.1588 mL 0.3970 mL
    30 mM 0.0132 mL 0.0662 mL 0.1323 mL 0.3308 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tat-NR2B9c500992-11-0Tat-NR2Bct NA-1NA1NA 1NA-1iGluRNO SynthaseIonotropic glutamate receptorsNitric oxide synthasesNOSInhibitorinhibitorinhibit

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