Chlorpromazine (CPZ) (10 µM; 30 min) remarkably decreases the cellular internalization of FITC-P-LPK in HCT116 cells. Chlorpromazine can inhibit clathrin-mediated endocytosis. The fluorescence intensity is observed by confocal microscopy and detected using a multi-well plate reader.
Chlorpromazine is an orally active, blood-brain barrier-transparent antipsychotic agent that effectively antagonises D2 dopamine receptors and 5-HT2A, which is widely used in schizophrenia and other psychiatric disorders. Chlorpromazine exerts anti-cancer activity through a variety of pathways, including anti-proliferation, induction of autophagy and cycle arrest (G2-M phase), inhibition of cytochrome c oxidase (CcO), inhibition of tumour growth and metastasis, and inhibition of tumour immune escape. Chlorpromazine also blocks hNav1.7 channels (IC50=25.9 μM; concentration-dependent) and HERG potassium channels (IC50=21.6 μM), which has potential for analgesic and cardiac arrhythmic studies. Chlorpromazine also can inhibit clathrin-mediated endocytosis[1][2][3][4][5].
Chlorpromazine (0, 10, 20, 40 μM; 0, 24, 48 h) inhibits growth of U-87MG glioma cells in a dose- and time- dependent manner[2].
Chlorpromazine (20 μM; 0, 12, 24, 48 h) decreases the levels of cyclin A and cyclin B1 in U-87MG glioma cells, 12 h later[2].
Chlorpromazine (20 μM) causes inhibition of cell cycle progression[2].
Chlorpromazine (10 μM; 1 h) significantly suppresses the sEV internalization and dramatically reduces MDSCs in sEV-treated bone marrow cells (MDSCs can significantly suppress the immune cell response, causing immunosuppression in cancer cells)[3].
Chlorpromazine (3, 10, 20, 40, 60 μM) blocks the hNav1.7 current in a concentration-dependent manner[4].
Chlorpromazine blocks HERG potassium channels with an IC50 value of 21.6 μM and a Hill coefficient of 1.11[5].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Decreased the levels of cyclin A and cyclin B1 12 h later, whereas levels of cyclin D1, proliferating cell nuclear antigen and GAPDH remained unchanged.
体内研究 (In Vivo)
Chlorpromazine (20 mg/kg; i.p.; single daily for 7 days) inhibits xenograft tumor growth in nude mouse[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
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可选溶剂
浓度溶剂体积质量
1 mg
5 mg
10 mg
25 mg
DMSO
1 mM
3.1362 mL
15.6809 mL
31.3617 mL
78.4043 mL
5 mM
0.6272 mL
3.1362 mL
6.2723 mL
15.6809 mL
10 mM
0.3136 mL
1.5681 mL
3.1362 mL
7.8404 mL
15 mM
0.2091 mL
1.0454 mL
2.0908 mL
5.2270 mL
20 mM
0.1568 mL
0.7840 mL
1.5681 mL
3.9202 mL
25 mM
0.1254 mL
0.6272 mL
1.2545 mL
3.1362 mL
30 mM
0.1045 mL
0.5227 mL
1.0454 mL
2.6135 mL
40 mM
0.0784 mL
0.3920 mL
0.7840 mL
1.9601 mL
50 mM
0.0627 mL
0.3136 mL
0.6272 mL
1.5681 mL
60 mM
0.0523 mL
0.2613 mL
0.5227 mL
1.3067 mL
80 mM
0.0392 mL
0.1960 mL
0.3920 mL
0.9801 mL
100 mM
0.0314 mL
0.1568 mL
0.3136 mL
0.7840 mL
Help & FAQs
Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
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