EF24

目录号: HY-119272 纯度: 99.78%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

EF24 是一种姜黄素类似物，具有更强的抗肿瘤功效和口服生物利用度。在口腔鳞状细胞癌细胞中，EF24 通过抑制 MAPK/ERK 信号通路发挥抗肿瘤作用。EF24 处理可提高活化的 Caspase3 和 Caspase9 的水平，并降低 MEK1 和 ERK 的磷酸化形式的表达。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

EF24 Chemical Structure

CAS No. : 342808-40-6

1. 客户无需承担相应的运输费用。

2. 同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

可免费申领三个不同产品的试用装。

3. 试用装只面向终端客户。

规格	价格	是否有货
10 mM * 1 mL in DMSO	￥1210	In-stock
5 mg	￥1100	In-stock
10 mg	￥1950	In-stock
25 mg	￥4100	In-stock
50 mg	￥5750	In-stock
100 mg	现货	询价
200 mg		询价
500 mg		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

MCE 顾客使用本产品发表的 1 篇科研文献

•Phytomedicine. 2024 Jul 27:133:155882. [Abstract]

EF24 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 ERK 亚型特异性产品:

查看所有亚型

ERK ERK1 ERK2 ERK5 ERK8

查看 Caspase 亚型特异性产品:

查看所有亚型

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

生物活性
纯度 & 产品资料
参考文献

生物活性

EF24 is a curcumin analogue with greater anti-tumor efficacy and oral bioavailability via deactivation of the MAPK/ERK signaling pathway in oral squamous cell carcinoma (OSCC). EF24 treatment increases the levels of activated caspase 3 and 9, and decreases the phosphorylated forms of MEK1 and ERK^[1]^[2].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
518A2	IC₅₀	1.8 μM Compound: 2; EF24	Cytotoxicity against human 518A2 cells after 72 hrs by MTT assay Cytotoxicity against human 518A2 cells after 72 hrs by MTT assay	[PMID: 28774574]
A2780	IC₅₀	0.5 μM Compound: EF24	Cell viability of human cisplatin-sensitive human A2780 cells after 24 hrs by MTT assay Cell viability of human cisplatin-sensitive human A2780 cells after 24 hrs by MTT assay	[PMID: 17684018]
A2780	IC₅₀	0.65 μM Compound: EF24	Cell viability of cisplatin-resistant human A2780 cells after 24 hrs by MTT assay Cell viability of cisplatin-resistant human A2780 cells after 24 hrs by MTT assay	[PMID: 17684018]
A549	IC₅₀	2.4 μM Compound: EF24	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
A549	IC₅₀	2.8 μM Compound: A1; EF24	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 29351887]
A549	IC₅₀	7.1 μM Compound: EF24	Cytotoxicity against human A549 cells measured after 72 hrs by MTT assay Cytotoxicity against human A549 cells measured after 72 hrs by MTT assay	[PMID: 27886548]
CHO	IC₅₀	4.6 μM Compound: EF24	Cell viability of CHO cells after 24 hrs by MTT assay Cell viability of CHO cells after 24 hrs by MTT assay	[PMID: 17684018]
DLD-1	IC₅₀	1.3 μM Compound: 2; EF24	Cytotoxicity against human DLD1 cells after 72 hrs by MTT assay Cytotoxicity against human DLD1 cells after 72 hrs by MTT assay	[PMID: 28774574]
HCT-116	IC₅₀	1.5 μM Compound: 2; EF24	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 28774574]
HEK293	CC₅₀	3.58 μM Compound: 33	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability at 20 uM measured after 48 hrs by resazurin reagent based multilabel reader analysis Cytotoxicity against human HEK293 cells assessed as reduction in cell viability at 20 uM measured after 48 hrs by resazurin reagent based multilabel reader analysis	[PMID: 36608774]
HeLa 229	IC₅₀	1.9 μM Compound: A1; EF24	Antiproliferative activity against human HeLa 229 cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa 229 cells after 72 hrs by MTT assay	[PMID: 29351887]
HepG2	IC₅₀	3.33 μM Compound: EF24	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 30771605]
HT-29	IC₅₀	1.6 μM Compound: 2; EF24	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 28774574]
L02	IC₅₀	10.2 μM Compound: EF24	Cytotoxicity against human LO2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human LO2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 30771605]
L02	IC₅₀	5.1 μM Compound: EF24	Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
L02	IC₅₀	5.8 μM Compound: EF24	Antiproliferative activity against human HL7702 cells by MTT assay Antiproliferative activity against human HL7702 cells by MTT assay	[PMID: 31336310]
Lewis lung carcinoma cell line	IC₅₀	8.4 μM Compound: EF24	Cytotoxicity against mouse LLC cells measured after 72 hrs by MTT assay Cytotoxicity against mouse LLC cells measured after 72 hrs by MTT assay	[PMID: 27886548]
MGC-803	IC₅₀	2 μM Compound: A1; EF24	Antiproliferative activity against human MGC803 cells after 72 hrs by MTT assay Antiproliferative activity against human MGC803 cells after 72 hrs by MTT assay	[PMID: 29351887]
NB-4	IC₅₀	0.29 μM Compound: 42	Cytotoxicity against human NB4 cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against human NB4 cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 24960549]
NCI-H1650	IC₅₀	1.7 μM Compound: EF24	Cytotoxicity against human NCI-H1650 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human NCI-H1650 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
NCI-H1650	IC₅₀	14.6 μM Compound: EF24	Cytotoxicity against human NCI-H1650 cells measured after 72 hrs by MTT assay Cytotoxicity against human NCI-H1650 cells measured after 72 hrs by MTT assay	[PMID: 27886548]
NCI-H1975	IC₅₀	1.3 μM Compound: EF24	Cytotoxicity against human NCI-H1975 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human NCI-H1975 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
NCI-H441	IC₅₀	44 μM Compound: 1	Antiproliferative activity against human H441 cells assessed as decrease of hexosaminidase activity after 24 hrs Antiproliferative activity against human H441 cells assessed as decrease of hexosaminidase activity after 24 hrs	[PMID: 20638855]
NCI-H460	IC₅₀	1.2 μM Compound: EF24	Cytotoxicity against human H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
NCI-H460	IC₅₀	6 μM Compound: EF24	Antiproliferative activity against human H460 cells by MTT assay Antiproliferative activity against human H460 cells by MTT assay	[PMID: 31336310]
PANC-1	IC₅₀	1.5 μM Compound: 2; EF24	Cytotoxicity against human PANC1 cells after 72 hrs by MTT assay Cytotoxicity against human PANC1 cells after 72 hrs by MTT assay	[PMID: 28774574]
QGY-7703	IC₅₀	4.97 μM Compound: EF24	Cytotoxicity against human QGY7703 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human QGY7703 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 30771605]
SGC-7901	IC₅₀	1 μM Compound: A1; EF24	Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay	[PMID: 29351887]
SGC-7901	IC₅₀	6.5 μM Compound: EF24	Antiproliferative activity against human SGC7901 cells by MTT assay Antiproliferative activity against human SGC7901 cells by MTT assay	[PMID: 31336310]
SMMC-7721	IC₅₀	8.8 μM Compound: EF24	Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 30771605]
U-251	IC₅₀	4.5 μM Compound: EF24	Antiproliferative activity against human U251 cells by MTT assay Antiproliferative activity against human U251 cells by MTT assay	[PMID: 31336310]
U-87MG ATCC	IC₅₀	1.4 μM Compound: 2; EF24	Cytotoxicity against human U87 cells after 72 hrs by MTT assay Cytotoxicity against human U87 cells after 72 hrs by MTT assay	[PMID: 28774574]

分子量

311.33

Formula

C₁₉H₁₅F₂NO

CAS 号

342808-40-6

性状

固体

颜色

White to yellow

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (160.60 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.2120 mL	16.0601 mL	32.1203 mL
5 mM	0.6424 mL	3.2120 mL	6.4241 mL
10 mM	0.3212 mL	1.6060 mL	3.2120 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.03 mM); 悬浊液; 超声助溶

此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.03 mM); 悬浊液; 超声助溶

此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.78%

选择批次：

Data Sheet (622 KB) SDS (251 KB)

COA (261 KB) HNMR (255 KB) LCMS (269 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Lelli D, et al. Curcumin and treatment of melanoma: The potential role of microRNAs. Biomed Pharmacother. 2017 Apr;88:832-834. [Content Brief]

[2]. Mosley CA, et al. Highly active anticancer curcumin analogues. Adv Exp Med Biol. 2007;595:77-103. [Content Brief]

EF24 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2120 mL	16.0601 mL	32.1203 mL	80.3006 mL
	5 mM	0.6424 mL	3.2120 mL	6.4241 mL	16.0601 mL
	10 mM	0.3212 mL	1.6060 mL	3.2120 mL	8.0301 mL
	15 mM	0.2141 mL	1.0707 mL	2.1414 mL	5.3534 mL
	20 mM	0.1606 mL	0.8030 mL	1.6060 mL	4.0150 mL
	25 mM	0.1285 mL	0.6424 mL	1.2848 mL	3.2120 mL
	30 mM	0.1071 mL	0.5353 mL	1.0707 mL	2.6767 mL
	40 mM	0.0803 mL	0.4015 mL	0.8030 mL	2.0075 mL
	50 mM	0.0642 mL	0.3212 mL	0.6424 mL	1.6060 mL
	60 mM	0.0535 mL	0.2677 mL	0.5353 mL	1.3383 mL
	80 mM	0.0402 mL	0.2008 mL	0.4015 mL	1.0038 mL
	100 mM	0.0321 mL	0.1606 mL	0.3212 mL	0.8030 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EF24342808-40-6EF 24EF-24ERKCaspaseExtracellular signal regulated kinasesInhibitorinhibitorinhibit

您最近查看的产品:

产品名称：

* 需求量：

* 客户姓名：

* Email：

* 电话：

* 公司或机构名称：

留言给我们：

MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。	站点地图隐私声明
沪ICP备15051369号-4 上海工商沪公网安备31011502019417 沪(浦)应急管危经许[2021]201709(QFYS) 营业执照（三证合一）
Copyright © 2013-2024 MedChemExpress. All Rights Reserved.

MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

EF24

Customer Review

MCE 顾客使用本产品发表的 1 篇科研文献