2. 天然产物
  3. 植物 疾病研究领域 其他类
  4. 菊科 炎症/免疫 唇形科
  5. Baccharis darwinii Hook. & Arn.
  6. Enmein

Enmein 从 I. serra 中分离，具有免疫抑制作用。

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Enmein Chemical Structure

Enmein Chemical Structure

CAS No. : 3776-39-4

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Customer Review

Enmein 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Enmein is isolated from I. serra with immunosuppressive effect[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
> 10 μM
Compound: 17
Cytotoxicity against human A549 cells after 48 hrs
Cytotoxicity against human A549 cells after 48 hrs
[PMID: 19697926]
A549 IC50
> 40 μM
Compound: 8
Cytotoxicity against human A549 cells after 48 hrs by MTS assay
Cytotoxicity against human A549 cells after 48 hrs by MTS assay
[PMID: 24219809]
A549 IC50
12.07 μM
Compound: 3
Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 30921757]
Bel-7402 IC50
14.18 μM
Compound: 3
Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 30921757]
HepG2 IC50
2.02 μM
Compound: 17
Cytotoxicity against human HepG2 cells after 48 hrs
Cytotoxicity against human HepG2 cells after 48 hrs
[PMID: 19697926]
HL-60 IC50
> 40 μM
Compound: 8
Cytotoxicity against human HL60 cells after 48 hrs by MTS assay
Cytotoxicity against human HL60 cells after 48 hrs by MTS assay
[PMID: 24219809]
HL-60 IC50
19.52 μM
Compound: 3
Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 30921757]
K562 IC50
1.62 μM
Compound: 17
Cytotoxicity against human K562 cells after 48 hrs
Cytotoxicity against human K562 cells after 48 hrs
[PMID: 19697926]
K562 IC50
2.76 μM
Compound: 3
Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 30921757]
L02 IC50
25.47 μM
Compound: 3
Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 30921757]
MCF7 IC50
> 40 μM
Compound: 8
Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay
Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay
[PMID: 24219809]
PC-3 IC50
19.35 μM
Compound: 3
Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 30921757]
SGC-7901 IC50
26.23 μM
Compound: 3
Antiproliferative activity against human SGC7901 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Antiproliferative activity against human SGC7901 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 30921757]
SMMC-7721 IC50
> 40 μM
Compound: 8
Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay
Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay
[PMID: 24219809]
SW480 IC50
> 40 μM
Compound: 8
Cytotoxicity against human SW480 cells after 48 hrs by MTS assay
Cytotoxicity against human SW480 cells after 48 hrs by MTS assay
[PMID: 24219809]
体外研究
(In Vitro)

Enmein shows significant inhibitory effect toward K562 with an IC50 value ranging from 3.2 μg/ml to 8.2 μg/ml[2].
Enmein (3.13-25 μg/ml; 48 hours) has inhibitory effect on lymphocytes with significant difference within concentration from 6.25 μg/ml to 25 μg/ml[1].
Enmein (5.5-22 μg/ml; 48 hours) causes the percentage of S stage cells decreased from 19% to 6%, causes the percentage of G2/M stage cells to decrease from 2% to 0.4%[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Enmein 相关抗体:

Cell Viability Assay[1]

Cell Line: Splenic lymphocyte cells
Concentration: 3.13, 6.25, 12.5, 25 μg/ml
Incubation Time: 48 hours
Result: Exhibited an IC50 value of 17 μg/ml in splenic lymphocytes.

Cell Cycle Analysis[1]

Cell Line: Splenic lymphocyte cells
Concentration: 5.5 μg/ml, 11 μg/ml, 22 μg/ml
Incubation Time: 48 hours
Result: Caused S stage cells and G2/M stage cells decreased.
体内研究
(In Vivo)

Enmein (inject to ear; 6.52-13.04 mg/kg; 5 days) inhibits the xylene-induced ear swelling and inhibits IL-2 expression[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/c mice[1]
Dosage: 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg
Administration: Injected to ear; 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg; 5 days
Result: Had inhibitory effect on IL-2 release in blood.
分子量

362.42

Formula

C20H26O6
CAS 号
性状

固体

颜色

White to off-white

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (275.92 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7592 mL 13.7961 mL 27.5923 mL
5 mM 0.5518 mL 2.7592 mL 5.5185 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.90 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.90 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: ≥98.0%

COA (297 KB)

产品使用指南 (1538 KB)
参考文献

Enmein 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7592 mL 13.7961 mL 27.5923 mL 68.9807 mL
5 mM 0.5518 mL 2.7592 mL 5.5185 mL 13.7961 mL
10 mM 0.2759 mL 1.3796 mL 2.7592 mL 6.8981 mL
15 mM 0.1839 mL 0.9197 mL 1.8395 mL 4.5987 mL
20 mM 0.1380 mL 0.6898 mL 1.3796 mL 3.4490 mL
25 mM 0.1104 mL 0.5518 mL 1.1037 mL 2.7592 mL
30 mM 0.0920 mL 0.4599 mL 0.9197 mL 2.2994 mL
40 mM 0.0690 mL 0.3449 mL 0.6898 mL 1.7245 mL
50 mM 0.0552 mL 0.2759 mL 0.5518 mL 1.3796 mL
60 mM 0.0460 mL 0.2299 mL 0.4599 mL 1.1497 mL
80 mM 0.0345 mL 0.1725 mL 0.3449 mL 0.8623 mL
100 mM 0.0276 mL 0.1380 mL 0.2759 mL 0.6898 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Enmein3776-39-4OthersInhibitorinhibitorinhibit

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